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C483 Study Guide for Exam 3
Spring 2017
Basic Information
 Exam 3: Thursday, April 20, 7:15-9:15PM in Rawles 100
 Arrive early for assigned seats
 Bring your student ID. Failure to do so will result in getting your exam back later.
 You may use a NON-PROGRAMMABLE calculator.
 All papers, books, phones, and electronic devices must be in a sealed bag under your seat.
The exam will cover chapters 13-15 and 17, which includes
 Glycolysis, gluconeogenesis, glycogen metabolism, pentose phosphate pathway
 Pyruvate dehydrogenase complex, citric acid cycle
 Electron transport chain, protonmotive force, oxidative phosphorylation
 Fatty acid degradation and synthesis, cholesterol synthesis and processing
Major topics
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Stages/major purpose/net reaction for pathways
Intermediates of glycolysis, gluconeogenesis, citric acid cycle, glycogen metabolism,
fatty acid metabolism
o Structure and name of intermediates and enzymes
o Thermodynamics of each step, qualitative and quantitative
o Major regulation steps; reciprocal regulation strategies
o Points of interconnection between pathways
o Amount of ATP/reduced cofactor produced in each step/pathway
o Cofactors needed for transformation
Arrow mechanisms if mechanism is given in class
o NAD+/NADH redox (lactate DH, glucose-6-phosphate DH, etc)
o Isomerases (phosphoglucose, triose phosphate, etc)
o Aldolase
o GAP DH (using the structure of NAD+)
o Pyruvate carboxylase
o Pyruvate DH (TPP step)
o Citrate synthase
o Compare/contrast isocitrate DH and -ketoglutarate DH
o transketolase
Pentose phosphate pathway: know phases, purposes, alternate modes, which type of
enzyme needed for a given transformation, transketalase mechanism
Electron transport chain/oxidative phosphorylation
o Order of main complexes and mobile carriers (not details of complexes)
o Number of protons pumped
o Qualitative/quantitative redox reactions
o P:O ratio
o Basic mechanism of binding-change ATP production