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Transcript
Eurobiofilms 2015
Fourth European Congress on Microbial Biofilms – Basic and Clinical Aspects
(PHOTOS)
Brno, Czech Republic, 23-26 June, 2015
www.eurobiofilms2015.ics.dk
Final Programme
Table of Contents
Welcome Message ..................................................................................................... 3
Organisation.............................................................................................................. 3
Programme Overview...........................................................................................4 – 7
Scientific Programme ..................................................................................... 13 – 20
Information to Presenters ..........................................................................................8
Sponsors and Support ............................................................................................... 8
Registration ............................................................................................................ 10
Welcome Message
On behalf of the ESCMID Study Group on Biofilms (ESGB) and the Organising Committee, it is our great pleasure
to welcome you to the EUROBIOFILMS 2015 meeting in Brno. This meeting follows the tradition started with the
EUROBIOFILMS meetings organised in 2009 (Rome, Italy) and 2011 (Copenhagen, Denmark) and 2013 (Ghent,
Belgium).
It is clear from the number of submitted abstracts and registered participants that microbial biofilms continue to be
a hot topic among microbiologists. This conference provides a platform for all scientists studying basic, clinical and
environmental aspects of microbial biofilms to present novel data and to exchange new information on bacterial
and fungal biofilms. The multidisciplinary program covers many topics, and is balanced between basic and clinical aspects of microbial biofilms. Despite the busy program, we hope that you will find some time to catch up with
old friends and/or make some new friends and also to visit Brno, the city, where Johan Gregor Mendel took up
his genetic research. Let the city of Johan Gregor Mendel inspire you and your research and enjoy your stay on
EUROBIOFILMS 2015 in Brno!
Finally, we would like to thank the Czechoslovak Society for Microbiology (CSSM) and the Faculty of Medicine of
Masaryk University for their support and help with the congress organisation. In addition, support from ESCMID
has allowed us to provide attendance grants to 10 participants. Without these partners the organisation of this
meeting would not have been possible.
Veronika Holá
(President)
Filip Růžička
(Vice-president)
Organisation
Martin Rulík
(Vice-president)
Congress Secretariat
Organising Committee:
Veronika Holá (president), Filip Růžička (vice-president, medical biofilms), Martin Rulík (vice-president, natural biofilms), Monika DvořákováHeroldová, Martina Mahelová, Tereza Peroutková, Petra Šišková, Ondřej
Zahradníček and the ESGB Executive Committee
Scientific Committee:
Thomas Bjarnsholt (DK), Helena Bujdakova (SK), Mette Burmølle (DK),
Henk Busscher (NL), Tom Coenye (BE), Steve Diggle (UK), Gianfranco
Donelli (IT), Luanne Hall-Stoodley (USA), Niels Høiby (DK), Veronika
Holá (CZ), Christine Imbert (FR), Bastiaan Krom (NL), Andrew McBain
(UK), Thomas Neu (DE), Alex O’Neill (UK), Antonio Oliver (ES), Martin
Rulík (CZ), Kendra Rumbaugh (USA), Filip Růžička (CZ), Mark Shirtliff
(USA), Phil Stewart (USA), Paul Stoodley (USA), David Stopar (SLO),
Claudia Vuotto (IT), Craig Williams (UK)
International Conference Services
P.O. Box 41
Strandvejen 169-171
DK-2900 Hellerup, Copenhagen
Denmark
ESGB (ESCMID Study Group for Biofilms) Executive Committee:
Thomas Bjarnsholt (DK, chairperson), Tom Coenye (BE, vice chairperson), Christine Imbert (FR, secretary), Craig Williams (UK, treasurer),
Veronika Hola (CZ), Antonio Oliver (ES)
Tel: +45 7023 5056
E-mail: [email protected]
Web: www.eurobiofilms2015.ics.dk
Web: www.ics.dk
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Programme Overview
TUESDAY, 23 JUNE 2015
Time
09:00 – 16:00
MEETING ROOM A11/311
MEETING ROOM A11/327
W01 – Educational Workshop
Growth and imaging techniques for laboratory
and clinical biofilm research
Directors: Paul Stoodley, (USA) & Claus Sternberg, (Denmark)
Biofilm laboratory growth systems – an overview
Paul Stoodley, (The Ohio State University, Department of Microbial Infection and Immunity, Columbus, OH, USA)
Working with clinical and animal specimen
Thomas Bjarnsholt, (University of Copenhagen,
Faculty of Health Sciences, Department of International Health, Immunology & Microbiology, Copenhagen, Denmark)
Biofilm flow cells – principles and assembly
(lecture and hands on)
Paul Stoodley, (USA)
Staining biofilms for imaging – live/dead, PNA
FISH (lecture and hands on mock demo)
Kasper Nørskov Kragh, (University of Copenhagen,
Department of Systems Biology, Copenhagen,
Denmark)
Rendering confocal biofilm images (Imaris –
lecture and hands on)
Claus Sternberg, (Technical University of Denmark,
Department of Systems Biology Lyngby, Denmark)
Techniques for quantifying biofilm for confocal
images (COMSTAT – lecture and hands on)
Claus Sternberg, (Denmark)
Panel Discussion
Participants will learn the principles behind the use of
various experimental systems to grow and image biofilms as well as techniques to image biofilms on clinical and ex vivo animal samples. There will be hands
on demonstrations of how to assemble a basic flow
cell system, perform PNA FISH and live / dead viability staining and how to best present and quantify your
confocal images using Imaris and COMSTAT imaging
software. There will be plenty of opportunity for interactive discussion between participants and workshop
faculty during the workshop.
Time
16:00 – 17:15
W02 – Educational Workshop
Experimental biofilm infection in animals
Directors: Thomas Bjarnsholt, (Denmark)
From in vitro to in vivo – what to consider Thomas Bjarnsholt, (University of Copenhagen, Faculty
of Health Sciences, Department of International
Health, Immunology & Microbiology, Copenhagen,
Denmark)
Animal experiments in general – legislation and
experimental designs
Claus Moser, (Rigshospitalet, Department of
Clinical Microbiology, Copenhagen, Denmark)
Evaluation of tolerance and resistance to antibiotics in animal experiments
Oana Ciofu, (ISIM, University of Copenhagen,
Faculty of Health and Medical Sciences, Copenhagen, Denmark)
Evaluation of inflammation and immune response in animal experiments
Peter Østrup Jensen, (Rigshospitalet, Department
of Clinical Microbiology, Copenhagen, Denmark)
Anti-biofilm vaccine studies in animals
Mark Shirtliff, (University of Maryland, Department
of Microbial Pathogenesis, Baltimore, MD, USA)
Transcriptomics and Tn-Seq in animal models
Kendra Rumbaugh, (Texas Tech University Health
Sciences Center – TTUHSC, School of Medicine,
Department of Surgery – The Rumbaugh Lab,
Lubbock, TX, USA) and
Marvin Whiteley, (The University of Texas, Department of Molecular Biosciences, Austin, TX, USA)
Lung and chronic wound models
Claus Moser, (Denmark)
Peritoneal implant and filler models
Thomas Bjarnsholt, (Denmark)
Osteomyelitis and dual infection models
Mark Shirtliff, (USA)
AULA AUDITORIUM “Hall A22/116”
Opening Ceremony
Chairs: Veronika Holá, (Czech Republic) & Filip Růžička, (Czech Republic)
Welcome
Veronika Holá, (EUROBIOFILMS 2015 Congress President, Czech Republic)
Welcome
Thomas Bjarnsholt, (Chairman of ESGB, Denmark)
Thematic issues of P&D
Tom Coenye, (Ghent University, Laboratory of Pharmaceutical Microbiology, Ghent, Belgium)
Opening Lecture
The Pel polysaccharide is a positively charged exopolysaccharide that cross-links extracellular DNA
in the Pseudomonas aeruginosa biofilm matrix
Matthew R Parsek, (University of Washington, Department of Microbiology, Seattle, WA, USA)
17:15 –
Opening Reception
-4-
WEDNESDAY, 24 JUNE 2015
Time
09:00 – 10:00
MEETING ROOM A11/114
KL01 – Keynote Lectures
Chairs: Thomas Bjarnsholt, (Denmark)
& Veronika Holá, (Czech Republic)
Insights into the function of microbial dark matters in environmental biofilms
Per Halkjær Nielsen, (Aalborg University, Department of Chemistry and Bioscience - Section for Biotech,
Aalborg, Denmark)
The importance of biofims in orthopedic implant-associated infections
Andrej Trampuz (Charité – University Medicine Berlin, Center for Musculoskeletal Surgery, Berlin, Germany)
10:00 – 10:30
Time
10:30 – 12:30
Coffee Break
MEETING ROOM A11/205
MEETING ROOM A11/114
S01 – Methods of detection & diagnosis of biofilm infections
Chairs: Luanne Hall-Stoodley, (USA) & Thomas
Thomas Bjarnsholt, (Denmark)
S02 – Mixed species fungal & bacterial biofilm
communities
Chairs: Bastiaan Krom, (The Netherlands)
& Thomas Neu, (Germany)
Methods of detection and diagnosis of biofilm
infections
Luanne Hall-Stoodley, (The Ohio State University
College of Medicine, Department of Microbial Infection and Immunity, Columbus, OH, USA)
Fungus-bacterium interactions in the healthy
oral cavity
Bastiaan Krom, (Academic Centre for Dentistry
Amsterdam (ACTA), Department of Preventive Dentistry, Amsterdam, The Netherlands)
Clinical and laboratory diagnosis of biofilm
infections
Craig Williams, (University of West Scotland & University Hospital Crosshouse, Institute of Healthcare
Associated Infection, Paisley, UK)
Lectin-barcoding of complex biofilms
Thomas Neu, (Helmholtz Center for Environmental
Research – UFZ, Department River Ecology, Magdeburg, Germany)
12:30 – 13:00
Lunch Break
13:00 – 15:00
Poster Session I
15:00 – 15:30
Coffee Break
15:30 – 17:30
S03 – Treatment of biofilm infections
Chairs: Antonio Oliver, (Spain) & Craig Williams,
(UK)
S04 – Social interactions in biofilms
Chairs: Tom Coenye, (Belgium) & Steve Diggle,
(UK)
Diagnosis and treatment of fungal biofilm
infection
Craig Williams, (University of West Scotland & University Hospital Crosshouse, Institute of Healthcare
Associated Infection, Paisley, UK)
Social interactions in staphylococcal biofilms:
metabolic changes in mixed S. aureus - S.
epidermidis biofilms and the role of QS in
antibiotic tolerance
Tom Coenye, (Ghent University, Laboratory of Pharmaceutical Microbiology, Ghent, Belgium)
The biofilm polysaccharide PSL is a social but
non-cheatable trait in Pseudomonas aeruginosa
Steve Diggle, (University of Nottingham, School of
Life Sciences, Nottingham, UK)
19:30 –
Organ and Children Choir Concert at the Cathedral of St. Peter and Paul (Petrov)
A guided tour through the Cathedral of St. Peter and Paul (Petrov) will follow just after the concert
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THURSDAY, 25 JUNE 2015
Time
09:00 – 10:00
MEETING ROOM A11/114
KL02 – Keynote Lectures
Chairs: Veronika Holá, (Czech Republic)
& Thomas Bjarnsholt, (Denmark)
Biofilm slime by design: viscoelastic yielding as a virulence factor for survival in chronic infections
Paul Stoodley, (The Ohio State University, Department of Microbial Infection and Immunity, Columbus, OH,
USA)
Looking for new perspectives to fight bacterial biofilm infections – identification and use of biofilmspecific functions
Jean-Marc Ghigo, (Pasteur Institute, Department of Genetics of Biofilms, Paris, France)
10:00 – 10:30
Time
10:30 – 12:30
Coffee Break
MEETING ROOM A11/132
MEETING ROOM A11/114
S05 – Host responses to biofilms
Chairs: Mark Shirtliff, (USA) & Niels Høiby,
(Denmark)
S06 – Biomechanics in biofilm and infections
Chairs: Phil Stewart, (USA) & Henk Busscher,
(The Netherlands)
The host response as an accomplice to monoand multi-species biofilm infections
Mark Shirtliff, (University of Maryland, Department
of Microbial Pathogenesis, Baltimore, MD, USA)
Mechanics of biofilm detachment in flowing
fluids
Phil Stewart, (Montana State University, Center for
Biofilm Engineering, Bozeman, MT, USA)
A taste for biofilms: how phagocytic cells
recognise biofilms
Gertrud M Hänsch, (University of Heidelberg, Department of Immunology, Heidelberg, Germany)
From bacterial vibration spectroscopy on adhering bacteria to visco-elasticity of biofilms
Henk Busscher, (The Kolff Institute at the University Medical Center Groningen (UMCG), Department of Biomedical Engineering, Groningen, The
Netherlands)
12:30 – 13:00
Lunch Break
13:00 – 15:00
Poster Session II
15:00 – 15:30
Coffee Break
15:30 – 17:30
S07 – Resistance in biofilms & novel approaches
to treatment
Chairs: Gianfranco Donelli, (Italy) & Helena
Bujdáková, (Slovak Republic)
S08 – Modelling of biofilms & regulation of
biofilm development
Chairs: David Stopar, (Slovenia) & Kendra
Rumbaugh, (USA)
Rationally designing nanomedicines for the
eradication of pulmonary biofilm infections
Katrien Forier, (Ghent University, Department of
Clinical Chemistry, Microbiology and Immunology,
Ghent, Belgium)
Gradual transition into a floating biofilm
David Stopar, (University of Ljubljana, Department of Food Science and Technology, Ljubljana,
Slovenia)
Photodynamic inactivation – a possible
alternative in eradication of resistant
microbial biofilms
Helena Bujdákova, (Comenius University In
Bratislava, Faculty of Natural Sciences Department of Microbiology and Virology, Bratislava,
Slovak Republic)
Modeling polymicrobial wound infections
Kendra Rumbaugh, (Texas Tech University Health
Sciences Center – TTUHSC, School of Medicine,
Department of Surgery – The Rumbaugh Lab,
Lubbock, TX, USA)
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FRIDAY, 26 JUNE 2015
Time
09:00 – 11:00
MEETING ROOM A11/132
MEETING ROOM A11/114
S09 – Probiotics and new natural & synthetic
anti-biofilm compounds
Chairs: Christine Imbert, (France) & Claudia
Vuotto, (Italy)
S10 – Horizontal gene transfer, evolution &
heterogeneity in microbial biofilms
Chairs: Alex O’Neill, (UK) & Mette Burmølle,
(Denmark)
Probiotics and new natural and synthetic antibiofilm compounds
Elizabeth Presterl, (Medical University of Vienna, Department of Internal Medicine I, Division of Infectious
Diseases and Tropical Medicine, Vienna, Austria)
Accelerated evolution of antibiotic resistance
in staphylococcal biofilms
Alex O’Neill, (University of Leeds, School of Molecular and Cellular Biology (SMCB), Leeds, UK)
Probiotics: a potential tool to counteract
biofilm-based infections
Claudia Vuotto, (IRCCS Fondazione Santa Lucia,
Microbial Biofilm Laboratory, Rome, Italy)
11:00 – 11:30
Time
11:30 – 13:30
Do plasmids drive their host into the biofilm
lifestyle and why would they?
Mette Burmølle, (University of Copenhagen,
Department of Biology, Copenhagen, Denmark)
Coffee Break
MEETING ROOM A11/132
S11 – Environmental biofilms and human health
Chairs: Andrew McBain, (UK) & Martin Rulík, (Czech Republic)
Microbicide-exposure and antimicrobial resistance in biofilms in the domestic environment
Andrew McBain, (University of Manchester, Manchester Pharmacy School, Manchester, UK)
Bacterial–Trojan Horse interactions: the breakthrough of the new approaches to their study
Katarina Trnková, (University of Matej Bel, Faculty of Natural Sciences, Department of The Environment,
Banská Bystrica, Slovak Republic)
Microfluidic high-throughput platform for biofilm analysis
Tereza Hájková, (BioTech Europe, Prague, Czech Republic)
13:30 –
Closing Ceremony
Poster Awards
-7-
Information to Presenters
Oral Presentations
Poster Presentations
Audio-visual equipment
All posters should be mounted on Wednesday, 24
June 2015 by 09:00 and will remain mounted for the
duration of the Congress.
The auditoria at the Congress Venue will be equipped
with PCs with Power Point software and data projectors.
All presentations need to be prepared with Microsoft
PowerPoint. All recent versions of Microsoft PowerPoint
are accepted.
Please bring your presentation on a USB stick (make
sure to have a backup somewhere!). All presenters are
strongly recommended to use the PCs provided by the
organisation and should upload their presentation in the
break before their session the latest, and preferably earlier. If you are bringing your own laptop, please check
that you are able to hook up your laptop to the video
projector at least in the break before your session. We
recommend you to bring your own electrical adapter as
well. There will be no Preview Room available.
Posters sessions will take place in two groups. Poster
Sessions PS01 – PS04 will be presented by the presenting author during the first Poster Session (Wednesday, 24 June 2015, from 13.00 to 15.00).
Poster Sessions PS05 – PS11 will be presented by the
presenting author during the second poster session
(Thursday, 25 June 2015, from 13.00 to 15.00).
You have been assigned a poster board number, date
and time. You are expected to be at your assigned space
on the day that your poster presentation is scheduled.
Presentation
Material to mount your poster will be provided.
Speakers are requested to present themselves to the
chair in the lecture room 10 minutes prior to the beginning of their session
 Please respect the time limits. Each presentation is
scheduled to last for 15 minutes, which means
approx. 10 minutes for the actual presentation
leaving 5 minutes for questions. The moderator is
responsible for the time table and will interrupt if a presentation extends beyond the allocated time.
All posters must be removed no later than the last break
on Friday, 26 June 2015. Remaining posters will be removed by the organisers, who cannot be held responsible for any damages.

Sponsors and Support
Organised and supported by ESCMID
The goal of ESGB is to promote and disseminate studies and knowledge about methods and results of biofilm
studies with relevance for infections in humans. The ultimate goal is to improve diagnostics and the results of prophylaxis and therapy of biofilm infections in humans. Please join us at: https://www.escmid.org/research_projects/
study_groups/biofilms/
With additional support from
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Registration
Registration Fees Onsite:
Registration & Information Desk
Regular participants € 700
Pre-graduates € 475
Opening hours:
Tuesday, 23 June
Wednesday, 24 June
Thursday, 25 June
Friday, 26 June
Entitlements
Delegates’ registration includes:
Participation in the Scientific Programme
Final Programme
Coffee and Lunch Breaks
Opening Reception
Organ and Children Choir Concert at the Petrov Cathedral
8:30 – 10.00/14:00-18:30
8:15-17:30
8:30-17:30
8:30-12:30
Cancellation & Refund Policy
Credit can not be given for unattended events, late arrivals
or early departure. All refunds will be processed after the
Congress.
Social Programme
Opening Reception
23 June 2015, 17:15 –
Come join your colleagues for an informal Opening Reception the evening before the first day of the official congress programme. The event
will take place at the congress venue.
Participation is included in the registration fee.
Evening concert in the Cathedral of St. Peter and Paul
(Petrov Cathedral)
The programme includes organ and choir compositions.
Organ: Petr Kolař
Choir: Children´s Choir of the National Theatre Brno directed by
Mrs. Klára Roztočilová
24 June 2015 at 19:30 –
The concert will be followed by a tour of the cathedral including the
bishops treasury and the cathedral towers.
Participation is included in the registration fee.
General Information
Congress Venue
Address:
Masaryk University Campus Conference Centre
Kamenice 5
Brno
Czech Republic
Masaryk University, the second oldest University in former Czechoslovak Republic, was established in January 1919, shortly after the creation of an independent
Czechoslovak state. Masaryk University was established with four faculties –
faculty of law, medicine, natural sciences and arts. From
the beginning it took the
name of the man who had done more than anyone else to bring it into existence: Tomáš Garrigue
Masaryk. The mission of the new university, as set out in the founding document of 1919, was
for it to “live, grow strong and flourish in noble competition with its older sister in Prague”. From
the very beginning, the university played an important role in the scientific, scholarly, cultural
and social life of the country. Its first honorary doctorate was awarded in 1925 to the composer
Leoš Janáček, a man with very close ties to Masaryk University. At the end of the 1930s, like
all other Czech universities, Masaryk University was closed down on 17 November 1939 by
the Nazi occupiers. According to an internal order issued by Protectorate command, the Brno
university was to be treated as though it had never existed. During the six wartime years, the
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university suffered incalculable losses. Worse than any material damage,
however, was the loss of professors and other university employees as a
direct result of Nazi persecution. The number of Masaryk University professors who were executed or tortured is exceptionally high; for instance, as
a consequence of Nazi terror, the Faculty of Science lost an entire quarter
of its teaching staff. The successful post-war renewal of university life was
brought to a halt by the Communist coup of 1948. The ensuing purges were
particularly difficult for the student population: the number of students expelled ranged from 5 % at the Faculty of Education to 46 % at the Faculty of
Law. The purge among employees was less drastic. In 1953 the Faculty of
Education (founded in 1946) was separated from the university and in 1960
Masaryk University reached the low point of its existence, being reduced
to only three faculties following the closure of the Pharmaceutical Faculty, the only institution of its kind in all of Bohemia
and Moravia. At the same time, the university lost its original name and was renamed to the Jan Evangelista Purkyně
University in Brno. It was only after the Velvet Revolution that the university awakened from a long period of stagnation. It
was renamed to Masaryk University in 1990. Now, the Masaryk University possesses eight faculties, in 2006 the Johann
Gregor Mendel Antarctic Station was established, in 2007 Mendeleum, the Mendel Museum, became a component part
of MU and in 2010 the University Campus Bohunice was opened.
Wireless internet connection will be available at the conference venue.
A2, A3, A6 Integrated Laboratories for
Biomedical Technologies (ILBIT)
A4 CEITEC MU and FSci laboratories
A5 Department of Biochemistry (FSci)
A7 Department of Biology (FMed)
A8 Department of Organic Chemistry (FSci)
A9 University Campus Library (UCL)
A10 Joint Educational Laboratories (FSci)
A11 Joint Educational Centre
A12 Department of Inorganic Chemistry and
Physical and Theoretical Chemistry (FSci)
A13 Department of Theoretical and Physical
Chemistry (FSci)
A14 Department of Analytical Chemistry (FSci)
A15 Biochemistry (FSci), MU Language Centre
A16 Department of Biochemistry (FMed)
A17 Dean’s Office (FMed), Administrative
Offices (FSci)
A18 Department of Pathological Physiology
(FMed)
A19 Department of Pharmacology (FMed),
Department of Medical Ethics (FMed)
UNIVERSITY CAMPUS
BOHUNICE 2014
www.kampus.muni.cz
NETROUFALKY STREET
TREET
EET
AC
PD
CRA
Bus Station
Univerzitní kampus - sever
S
NETROUFALKY STREET
STUDENTSKÁ STREET
A34
A26
G
A35
A25
A34
Bus Station
A32
A31
A36
A29
S
INBIT
EC
C
KAMENICE STREET
kampus
Bus Station
K
Kamenice
i
KAMENICE STREET
EC
8
A15
13
A14
A13
A12
A10
A5
4
AR
MAIN
ENTRANCE
F1
A22
1 2
12
3
A17
A11
A9
ETROUFALKY STREET
A19
Bus Station
Nemocnice Bohunice
(Bohunice Hospital)
A16
MC
A2
A7
A6
10
7
H
Z
6
A18
A3
9
A9
UCL
1
11
A20
A4
5
BCS
A21
AC
CRA
PD
Z
MC
EC
C
AR
SH
G
INBIT
BCS
H
SH
A33
1
A20 Department of Physiology (FMed)
A21 Department of Preventive Medicine
(FMed)
A22 Entrance hall, Assembly hall
A25, A31, A32, A36
CESEB – Biology buildings (FSci)
A26, A35 CEITEC
A29 CETOCOEN
A33 Dean’s Office (FSpS)
A34 Sports halls (FSpS)
A8
F2
H
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Academic canteen
Campus Residential Area
Projected residential development
Bio-model building
Morphology Centre (FMed)
Energy Centre
Footbridge with café
Academic restaurant MU
Sports hall
Gymnasiums
Incubator for Biomedical Technologies
Brno Campus Square
(accessed via footbridge F1)
University Hospital Brno
(accessed via footbridge F2)
Stadium (projected)
STATUE
1
Blades of Grass – Pavel Tasovský
2
T. G. Masaryk – Vincenc Makovský
3
Reliefplastik – Otto Herbert Hajek
4
Contacts and Distances – Aleš Veselý
5
The Flowering of Learning –
Vladimír Preclík
6
Enlightenment and Heavy Paint –
Lukáš Rittstein
7
Edward Babák – Vincenc Makovský
8
Balance – Jozef Jankovič
9
The Nest – Dalibor Chatrný
10
On Mary, On Eve – Olbram Zoubek
11
Three Figures – Michal Gabriel
12
Red is coming – Krištof Kintera
13
Inclined Circles – V. K. Novák
Transportation in Brno
Public Transporation
Using public transport in Brno is easy and convenient. Majority of trams, trolleybuses and buses are in service approx. 4:00-23:00 h, but some have shorter service. The interval between individual trams/buses on the same line
is 3–10 min in the peak time, 15–30 min in the evening.
During the night (23:00-4:00 h), special night buses circulate through the city (for more information, see www.dpmb.
cz or www.idsjmk.cz)
Fare: the traffic system designed for the whole South-Moravian region is based on the zones, but inside the city it
is not necessary to take care about them. Only time validity of your ticket is significant. Tickets should be bought in
advance in vending machines situated at many stops, in newsagents, at Brno Tourist Information Centre, at Brno
Public Transport Company information centres, in some hotels and via sms - Sejf application (app can be downloaded at https://play.google.com/store/apps/details?id=cz.edhouse.mc.android). Price of a ticket for 15 minutes is
20 CZK, for 60 minutes 25 CZK, for 90 minutes 27 CZK. It is also possible to buy one day ticket for 90 CZK or five
days ticket for 250 CZK. Some types of tickets are also sold by the driver, but they are something more expensive
and buyers are supposed to have exact sum to pay. If unable to show a valid ticket, you can be charged a fine of
up to CZK 1500 (ca. EUR 60)! The ticket can be used for all means of transport: trams, buses, and trolleybuses.
When entering the vehicle the ticket must be marked in the punching box.
About how to get to/from your hotels, Congress venue, Mendel Museum and Petrov Cathedral see detailed information online at http://eurobiofilms2015.ics.dk/.
Information on the Internet about:
Insurance
The Congress: www.eurobiofilms2015.ics.dk
The weather in Czech Republic: www.meteoprog.cz/en/
Masaryk University: www.muni.cz
Guide to Brno: www.gotobrno.cz
Transportation in Brno: www.dpmb.cz
You are requested to arrange your own health and
travel insurance. The congress organiser is not able to
take responsibility for any injuries or damages that may
occur during the congress.
Language
The official language of EUROBIOFILMS 2015 is English.
No translations will be provided.
Name Badge
Entrance to the sessions will be limited to badge holders
only. If the badge is lost, please contact the Registration
Desk. A fee of € 7 will be charged for each new badge.
Currency & Credit Cards
The currency in Czech Republic is Czech Crown
(CZK). Major credit cards are accepted in hotels,
restaurants and shops. The current exchange rate is
approx. 1 EUR = 27 CZK.
Using the credit card to pay a taxi is not available in all
taxis. Please check, if the driver accepts the credit card
payment before boarding.
-12-
Scientific Programme
TUESDAY, 23 JUNE 2015
08:30 –
Registration
MEETING ROOM A11/311
Time
09:00 – 16:00
W01
GROWTH AND IMAGING TECHNIQUES FOR LABORATORY AND CLINICAL
BIOFILM RESEARCH
Chairs: Paul Stoodley, (USA) & Claus Sternberg, (Denmark)
09:00 – 09:45
W01.1
Biofilm laboratory growth systems – an overview
Paul Stoodley, (USA)
09:45 – 10:30
W01.2
Working with clinical and animal specimens
Thomas Bjarnsholt, (Denmark)
10:30 – 10:45
Coffee Break
10:45 – 11:45
W01.3
Biofilm flow cells – principles and assembly
(Lecture and hands on)
Paul Stoodley, (USA)
11:45 – 12:45
W01.4
Staining biofilms for imaging – live/dead, PNA FISH
(Lecture and hands on mock demo)
Kasper Nørskov Kragh, (Denmark)
12:45 – 13:30
Lunch Break
13:30 – 14:30
W01.5
Rendering confocal biofilm images
(Imaris – lecture and hands on)
Claus Sterberg, (Denmark)
14:30 – 15:30
W01.6
Techniques for quantifying biofilm for confocal images
(COMSTAT – lecture and hands on)
Claus Sternberg, (Denmark)
15:30 – 16:30
Panel Discussion
MEETING ROOM A11/327
Time
09:00 - 16:00
W02
09:00 – 09:10
EXPERIMENTAL BIOFILM INFECTION IN ANIMALS
Chair: Thomas Bjarnsholt, (Denmark)
Welcome
09:10 – 09:45
W02.1
From in vitro to In vivo - what to consider
Thomas Bjarnsholt, (Denmark)
09:45 – 10:20
W02.2
Animal experiments in general – legislation and experimental
designs
Claus Moser, (Denmark)
10:20 – 10:45
10:45 – 11:20
Coffee Break
W02.3
Evaluation of tolerance and resistance to antibiotics in animal
experiments
Oana Ciofu, (Denmark)
-13-
TUESDAY, 23 JUNE 2015
MEETING ROOM A11/327
Time
09:00 – 16:00
W02
EXPERIMENTAL BIOFILM INFECTION IN ANIMALS (CONT.)
11:20 – 11:55
W02.4
Evaluation of inflammation and immune response in animal
experiments
Peter Østrup Jensen, (Denmark)
11:55 – 12:30
W02.5
Anti-biofilm vaccine studies in animals
Mark Shirtliff, (USA)
12:30 – 13:30
Lunch Break
13:30 – 14:05
W02.6
Transcriptomics and Tn-Seq in animal models
Kendra Rumbbaugh, (USA) & Marvin Whiteley, (USA)
14:05 – 14:40
W02.7
Lung and chronic wound models
Claus Moser, (Denmark)
14:40 – 15:15
W02.8
Peritoneal implant and filler models
Thomas Bjansholt, (Denmark)
15:15 – 15:30
15:30 – 16:00
Coffee Break
W02.9
Osteomyelitis and dual infection models
Mark Shirtliff, (USA)
AULA AUDITORIUM
Time
16:00 – 17:15
OPENING CEREMONY
Chairs: Veronika Holá, (Czech Republic) & Filip Růžička, (Czech Republic)
16:00 – 16:15
Welcome to EUROBIOFILMS 2015 by Veronika Holá, (Congress President)
16:15 – 16:30
Welcome by Thomas Bjarnsholt, (Chairman of ESGB)
16:30 – 16:45
Thematic issues of P&D
Tom Coenye, (Belgium)
16:45 – 17:15
17:15 –
OL
The Pel polysaccharide is a positively charged exopolysaccharide
that cross-links extracellular DNA in the Pseudomonas aeruginosa
biofilm matrix
Matthew R Parsek, (USA)
OPENING RECEPTION
-14-
WEDNESDAY, 24 JUNE 2015
08:30 –
Registration
MEETING ROOM A11/114
Time
09:00 – 10:00
KL01
KEYNOTE LECTURE
Chairs: Thomas Bjarnsholt, (Denmark) & Veronika Holá, (Czech Republic)
09:00 – 09:30
KL01.1
Insights into the function of microbial dark matters in
environmental biofilms
Per Halkjær Nielsen, (Denmark)
09:30 – 10:00
KL01.2
The importance of biofims in orthopedic implant-associated infections
Andrej Trampuz, (Germany)
10:00 – 10:30
Coffee Break
MEETING ROOM A11/205
Time
10:30 – 12:30
S01
METHODS OF DETECTION & DIAGNOSIS
OF BIOFILM INFECTIONS
Chairs: Luanne Hall-Stoodley, (USA) & Thomas Bjarnsholt, (Denmark)
10:30 – 11:00
S01.1
Methods of detection and diagnosis of biofilm infections
Luanne Hall-Stoodley, (USA)
11:00 – 11:30
S01.2
Clinical and laboratory diagnosis of biofilm infections
Craig Williams, (UK)
11:30 – 11:45
S01.3
Real-time biofilm monitoring in a multichannel online platform
Julia Bruchmann, (Germany)
11:45 – 12:00
S01.4
Rifampicin kills Staphylococcus epidermidis biofilm in hours
Rune Skjåstad, (Norway)
12:00 – 12:15
S01.5
The Italian Association of Clinical Microbiologists, (AMCLI)
protocol for the microbiological diagnosis of prosthetic joint
and fracture-fixation device infection
Iole Caola, (Italy)
12:15 – 12:30
S01.6
Human skin flora is heterogeneously distributed in biofilm
aggregates
Lene Bay, (Denmark)
MEETING ROOM A11/114
Time
10:30 – 12:30
S02
MIXED SPECIES FUNGAL & BACTERIAL BIOFILM COMMUNITIES
Chairs: Bastiaan Krom, (The Netherlands) & Thomas Neu, (Germany)
10:30 – 11:00
S02.1
Fungus-bacterium interactions in the healthy oral cavity
Bastiaan Krom, (The Netherlands)
11:00 – 11:30
S02.2
Lectin-barcoding of complex biofilms
Thomas R Neu, (Germany)
11:30 – 11:45
S02.3
Fungal β-1,3-glucan increases ofloxacin-tolerance of
Escherichia coli in a polymicrobial E. coli-Candida albicans biofilm
Katrijn De Brucker, (Belgium)
-15-
WEDNESDAY, 24 JUNE 2015
10:30 – 12:30
S02
MIXED SPECIES FUNGAL & BACTERIAL
BIOFILM COMMUNITIES (CONT.)
11:45 – 12:00
S02.4
Specific quantification of Pseudomonas aeruginosa in a
multispecies biofilm with PMA-qPCR
Sarah Tavernier, (Belgium)
12:00 – 12:15
S02.5
Extracellular matrix-mediated interspecies interactions mediate
co-aggregation and resistance during dual-species biofilm formation
Liang Yang, (Singapore)
12:15 – 12:30
S02.6
The cariogenic potential of Streptococcus mutans and
Candida albicans in an in vitro dual-species biofilm model
Hubertine ME Willems, (The Netherlands)
12:30 – 13:00
Lunch Break
13:00 – 15:00
POSTER SESSION I
MEETING ROOM A11/205
Time
15:30 – 17:30
S03
TREATMENT OF BIOFILM INFECTIONS
Chairs: Antonio Oliver, (Spain) & Craig Williams, (UK)
15:30 – 16:00
S03.1
Anti-biofilm properties of acetic acid
Thomas Bjarnsholt, (Denmark)
16:00 – 16:30
S03.2
Diagnosis and treatment of fungal biofilm infection
Craig Williams, (UK)
16:30 – 16:45
S03.3
Increased bactericidal activity of colistin on Pseudomonas
aeruginosa biofilms in anaerobic conditions
Mette Kolpen, (Denmark)
16:45 – 17:00
S03.4
Biofilm of endotracheal tubes from patients with MRSA
pneumonia treated with linezolid: preliminary results
Laia Fernandez-Barat, (Spain)
17:00 – 17:15
S03.5
Polydopamine-mediated immobilization of PALM and DNAse
to create an anti-adhesive and antimicrobial bi-functional coating
Diana Alves, (Portugal)
17:15 – 17:30
S03.6
Low dose nitric oxide as targeted anti-biofilm adjunctive therapy
to treat chronic Pseudomonas aeruginosa infection in cystic fibrosis
Jeremy S Webb, (UK)
MEETING ROOM A11/114
Time
15:30 – 17:30
S04
SOCIAL INTERACTIONS IN BIOFILMS
Chairs: Tom Coenye, (Belgium) & Steve Diggle, (UK)
15:30 – 16:00
S04.1
Social interactions in staphylococcal biofilms: metabolic changes in mixed S. aureus
- S. epidermidis biofilms and the role of QS in antibiotic tolerance
Tom Coenye, (Belgium)
16:00 – 16:30
S04.2
The biofilm polysaccharide PSL is a social but non-cheatable trait in Pseudomonas aeruginosa
Steve Diggle, (UK)
16:30 – 16:45
S04.3
Cooperative interactions in a four species biofilm community
Mette Burmølle, (Denmark)
16:45 – 17:00
S04.4
Selective signaling inhibition by extracellular polysaccharide alginate
Jiayue Yang, (Japan)
17:00 – 17:15
S04.5
Staphylocoagulase, an exploitable intra- and inter-specific public good
Urvish Trivedi, (Denmark)
17:15 – 17:30
S04.6
P. aeruginosa PAO1 provides grazing resistance to sensitive strains in a multispecies biofilm
Henriette Lyng Røder, (Denmark)
19:30 –
ORGAN AND CHILDREN CHOIR CONCERT AT THE PETROV CATHEDRAL
A GUIDED TOUR THROUGH THE PETROV CATHEDRAL
WILL FOLLOW JUST AFTER THE CONCERT
-16-
THURSDAY, 25 JUNE 2015
08:30 –
Registration
MEETING ROOM A11/114
Time
09:00 – 10:00
KL02
KEYNOTE LECTURE
Chairs: Veronika Holá, (Czech Republic) & Thomas Bjarnsholt, (Denmark)
09:00 – 09:30
KL02.1
Biofilm slime by design: viscoelastic yielding as a virulence
factor for survival in chronic infections
Paul Stoodley, (USA)
09:30 – 10:00
KL02.2
Looking for new perspectives to fight bacterial biofilm infections – identification
and use of biofilm-specific functions
Jean-Marc Ghigo, (France)
10:00 – 10:30
Coffee Break
MEETING ROOM A11/132
Time
10:30 – 12:30
S05
HOST RESPONSES TO BIOFILMS
Chairs: Mark Shirtliff, (USA) & Niels Høiby, (Denmark)
10:30 – 11:00
S05.1
The host response as an accomplice to mono- and
multi-species biofilm infections
Mark Shirtliff, (USA)
11:00 – 11:30
S05.2
A taste for biofilms: how phagocytic cells recognise biofilms
Gertrud Maria Hänsch, (Germany)
11:30 – 11:45
S05.3
S. epidermidis response to human blood and its cellular
and soluble components
Nuno Cerca, (Portugal)
11:45 – 12:00
S05.4
Dormancy with Staphylococcus epidermidis biofilms:
an immunoproteomic characterization
Virginia Carvalhais, (Portugal)
12:00 – 12:15
S05.5
Host defence against bacterial biofilms: activation of
defence-relevant functions by bacterial heat shock proteins
Ulrike Dapunt, (Germany)
12:15 – 12:30
S05.6
Extracellular dATP/ATP induces eDNA release and biofilm
formation in Candida albicans
Suma Pemmaraju, (India)
MEETING ROOM A11/114
Time
10:30 – 12:30
S06
BIOMECHANICS IN BIOFILM AND INFECTIONS
Chairs: Phil Stewart, (USA) & Henk Busscher, (The Netherlands)
10:30 – 11:00
S06.1
Mechanics of biofilm detachment in flowing fluids
Philip Stewart, (USA)
11:00 – 11:30
S06.2
From bacterial vibration spectroscopy on adhering bacteria
to visco-elasticity of biofilms
Henk J Busscher, (The Netherlands)
11:30 – 11:45
S06.3
Application of AFM for morphological, mechanical and
adhesive characterisation of microbial biofilms
Dimitar Stamov, (Germany)
-17-
THURSDAY, 25 JUNE 2015
10:30 – 12:30
S06
BIOMECHANICS IN BIOFILM AND INFECTIONS (CONT.)
11:45 – 12:00
S06.4
Developing a materials-based understanding of Bacillus subtilis
biofilm mechanics
James Wilking, (USA)
12:00 – 12:15
S06.5
An internal pushing force within a biofilm
Eric Raspaud, (France), C Douarche, J-M Allain
12:15 – 12:30
S06.6
Fluid forces to disrupt oral biofilms
Amir Rmaile, (The Netherlands), B Gottenbos, M de Jager, D Carugo, L Capretto,
M Aspiras, M Ward, P Stoodley
12:30 – 13:00
Lunch Break
13:00 – 15:00
POSTER SESSION II
MEETING ROOM A11/132
Time
15:30 – 17:30
S07
RESISTANCE IN BIOFILMS & NOVEL
APPROACHES TO TREATMENT
Chairs: Gianfranco Donelli, (Italy) & Helena Bujdákova, (Slovak Republic)
15:30 – 16:00
S07.1
Rationally designing nanomedicines for the eradication
of pulmonary biofilm infections
Katrien Forier, (Belgium)
16:00 – 16:30
S07.2
Photodynamic inactivation - a possible alternative in eradication
of resistant microbial biofilms
Helena Bujdakova, (Slovak Republic)
16:30 – 16:45
S07.3
Biological evaluation of an in vivo-potent dual target
PQS-Quorum Sensing inhibitor that hinders biofilm formation
Antonio Gurdjieff Gomes de Mello Martins, (Germany)
16:45 – 17:00
S07.4
Interplay between NO and H2S gasotransmitters in the
bacterial biofilm production and dispersion
Daria Streltsova, (Russian Federation)
17:00 – 17:15
S07.5
Type 1 fimbriae of multidrug resistant E. coli ST131: regulation,
function and inhibition using novel antivirulence therapeutics
Makrina Totsika, (Australia)
17:15 – 17:30
S07.6
Ability of Candida albicans to induce Staphylococcus aureus
biofilm tolerance to vancomycin
Sona Kucharikova, (Belgium)
MEETING ROOM A11/114
Time
15:30 – 17:30
S08
MODELLING OF BIOFILMS & REGULATION
OF BIOFILM DEVELOPMENT
Chairs: David Stopar, (Slovenia) & Kendra Rumbaugh, (USA)
15:30 – 16:00
S08.1
Gradual transition into a floating biofilm
David Stopar, (Slovenia)
16:00 – 16:30
S08.2
Modeling polymicrobial wound infections
Kendra Rumbaugh, (USA)
16:30 – 16:45
S08.3
Dangers of sticking together – biofilm aggregation in liquid batch cultures
Kasper Nørskov Kragh, (Denmark)
16:45 – 17:00
S08.4
New insights in Legionella pneumophila biofilm regulation by c-di-GMP signaling
Sophie Pecastaings, (France)
17:00 – 17:15
S08.5
Hydrophobic segments in exopolysaccharides of Burkholderia biofilms
Paola Cescutti, (Italy)
17:15 – 17:30
S08.6
Mineral scaffolds enable the morphogenesis of bacterial biofilms
Yaara Oppenheimer-Shaanan, (Israel)
-18-
FRIDAY, 26 JUNE 2015
08:30 –
Registration
MEETING ROOM A11/132
Time
09:00 – 11:00
S09
PROBIOTICS AND NEW NATURAL & SYNTHETIC
ANTI-BIOFILM COMPOUNDS
Chairs: Christine Imbert, (France) & Claudia Vuotto, (Italy)
09:00 – 09:30
S09.1
Probiotics and new natural and synthetic anti-biofilm compounds
Elisabeth Presterl, (Austria)
09:30 – 10:00
S09.2
Probiotics: a potential tool to counteract biofilm-based infections
Claudia Vuotto, (Italy)
10:00 – 10:15
S09.3
Inhibitory effect of flavonolignans on microbial biofilm formation
Vladimir Vimberg, (Czech Republic)
10:15 – 10:30
S09.4
Modulation of Staphylococcus aureus biofilm by metabolites
from Lactobacillus mucosae
Dobroslava Bujnakova, (Slovak Republic)
10:30 – 10:45
S09.5
Inhibitory effect of novel 1,2,4-triazole-cirofloxacin hybrids on
Haemophilus parainfluenzae biofilm in vitro
Anna Malm, (Poland)
10:45 – 11:00
S09.6
Lichens: promising source of inhibitors of Candida biofilms
Marion Girardot, (France)
MEETING ROOM A11/114
Time
09:00 – 11:00
S10
HORIZONTAL GENE TRANSFER, EVOLUTION &
HETEROGENEITY IN MICROBIAL BIOFILMS
Chairs: Alex O’Neill, (UK) & Mette Burmølle, (Denmark)
09:00 – 09:30
S10.1
Accelerated evolution of antibiotic resistance in
staphylococcal biofilms
Alex O’Neill, (UK)
09:30 – 10:00
S10.2
Do plasmids drive their host into the biofilm lifestyle
and why would they?
Mette Burmølle, (Denmark)
10:00 – 10:15
S10.3
Plasmid stability in biofilm vs. planktonic environments
Ida Thalsø-Madsen, (Denmark)
10:15 – 10:30
S10.4
c-di-GMP related genes are common on plasmids – a comparative analysis
Jonas Stenløkke Madsen, (Denmark)
10:30 – 10:45
S10.5
Helicobacter pylori outer membrane Vesicles, (OMVs) as
DNA “shuttles” and structural components of biofilm EPS
Rossella Grande, (Italy)
10:45 – 11:00
S10.6
Bacterial fortresses: secreted matrix structure determines
invasion resistance in bacterial biofilms
Knut Drecher, (Germany), C Nadell, N Wingreen, B Bassler
11:00 – 11:30
Coffee Break
-19-
FRIDAY, 26 JUNE 2015
MEETING ROOM A11/132
Time
11:30 – 13:30
S11
ENVIRONMENTAL BIOFILMS AND HUMAN HEALTH
Chairs: Andrew McBain, (UK) & Martin Rulík, (Czech Republic)
11:30 – 12:00
S11.1
Microbicide-exposure and antimicrobial resistance in biofilms
in the domestic environment
Andrew McBain, (UK)
12:00 – 12:30
S11.2
Bacterial-Trojan Horse interactions: the breakthrough of the
new approaches to their study
Katarína Trnková, (Slovak Republic)
12:30 – 12:45
S11.3
Microfluidic high-throughput platform for biofilm analysis
Tereza Hájková, (Czech Republic)
12:45 – 13:00
S11.4
Microbiome changing inside dental pipes: a case study
Damien Costa, (France)
13:00 – 13:15
S11.5
Activity and diversity of biofilm bacteria involved in
enhanced bioremediation of contaminated sediment
Birthe V Kjellerup, (USA)
13:15 – 13:30
S11.6
Effect of sodium hypochlorite on bacteria isolated from
drinking water
Inês B Gomes, (Portugal)
13:30 –
CLOSING CEREMONY & POSTER AWARDS
-20-
Poster Line Up
WEDNESDAY, 24 JUNE 2015
POSTER SESSION I
Time
13:00 – 15:00
13:00 – 15:00
PS01
METHODS OF DETECTION & DIAGNOSIS OF BIOFILM INFECTIONS
PS01.01
Real-time biofilm monitoring in a multichannel online platform
Julia Bruchmann, (Germany), K Sachsenheimer, BE Rapp, T Schwartz
PS01.02
Rifampicin kills Staphylococcus epidermidis biofilm in hours
Rune Skjåstad, (Norway), H Wiker, V Bakken
PS01.03
The Italian Association of Clinical Microbiologists (AMCLI) protocol
for the microbiological diagnosis of prosthetic joint and fracture-fixation device infection
Iole Caola, (Italy), L Drago, F Tessarolo, M Sarti, M Conte, E Zamparini,
P Viale, CL Romanò, P Clerici
PS01.04
Human skin flora is heterogeneously distributed in biofilm aggregates
Lene Bay, (Denmark), MS Ågren, SS Poulsen, T Bjarnsholt
PS01.05
Visualization of microbial biofilm in contaminated polymer tubes by X-ray micro-computed
tomography and contrast agents
Sascha Senck, (Austria), A Jäger, JM Grabmair, M Aigner, J Kastner
PS01.06
Investigation of different biofilm quantification methods in 96-well plates – applied for the
development of an enzymatic cleaner
Philipp Stiefel, (Switzerland), V Altenried, K Maniura-Weber, Q Ren
PS01.07
Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and
propidium iodide
Qun Ren, (Switzerland), P Stiefel, S Schmidt-Emrich, K Maniura-Weber
PS01.08
The dynamics of bacterial biofilm composition in patients with chronic periodontitis
Jiří Janata, (Czech Republic), L Šabová, L Najmanová, T Janatová, J Myšák,
J Dušková
PS01.09
A comparison of three eluting methods for dislodging biofilm and culturing
microorganisms from central venous catheters
Francesco Tessarolo, (Italy), F Piccoli, E Bonomi, P Lanzafame, G Nollo,
P Caciagli, I Caola
PS01.10
Alginate phenotypes of P. aeruginosa from Brazil and Scandinavia
Natália Candido Caçador, (Brazil), ALC Darini, LAGMM Torres,
O Ciofu, N Høiby
PS01.11
Progressive biofilm production several days post extubation: an experimental study with
MRSA isolates from oro-tracheally intubated pigs with pneumonia
Laia Fernandez-Barat, (Spain), S Benaicha, A Motos, A Mons, J Vila, A Torres
PS01.12
Fluorescent amphiphilic carbon dots as a new tool for visualization of Erwinia amylovora
exopolysaccharide matrix structure
Margarita Ritenberg, (Israel), S Kolusheva, R Jelinek
PS01.13
A rapid biofilm antimicrobial susceptibility assay and early antibiotic treatment in biofilms
Hengzhuang Wang, (Denmark), O Ciofu, N Høiby
PS01
METHODS OF DETECTION & DIAGNOSIS OF BIOFILM INFECTIONS (CONT.)
PS01.14
In vivo formation of Candida biofilms on catheters surfaces:
causes and infectivities
Sidi Mohammed Lahbib Seddiki, (Algeria), Z Boucherit-Otmani,
K Boucherit, AR Bettioui, M Taleb, D Kunkel
-21-
POSTER SESSION I
WEDNESDAY, 24 JUNE 2015
13:00 – 15:00
PS01.15
Biofilm detection by Raman spectrometry
Katarína Mlynáriková, (Czech Republic), O Samek, S Bernátová, J Ježek,
V Krzyžánek, M Šiler, P Zemánek, F Růžička, V Holá, M Mahelová
PS01.16
Biofilm formation on urinary catheters and other virulence factors of
the genus Proteus
Tereza Peroutková, (Czech Republic), V Holá, F Růžička
PS01.17
Flagellin (FliC) phosphorylation affects Type 2 protease secretion
and biofilm dispersal in Pseudomonas aeruginosa PAO1
Tanujaa Suriyanarayanan, (Singapore), S Periasamy, S Swarup
PS01.18
Presentation of an experimental system allowing exploring the first
steps of the biofilm formation at molecular level
Sebastien Vilain, (France), M Crouzet, P Costaglioli, C Barthe,
M Bonneu, B Garbay
PS01.19
Production of biofilm by Candida spp. isolated from bloodstream infections
Martina Mahelova, (Czech Republic), F Ruzicka
PS01.20
The effect of saccharide addition on biofilm production among
Escherichia coli
Petra Siskova, (Czech Republic), V Woznicova, L Cernohorska, F Růžička
PS01.21
Educational experimental kit for visualizing biofilm formation
Anne Kirstine Nielsen, (Denmark), PK Fridorff-Jens, T Bjarnsholt
PS01.22
Fungal b-1,3-glucan increases ofloxacin-tolerance of Escherichia coli in a polymicrobial
E. coli – Candida albicans biofilm
Katrijn De Brucker, (Belgium), Y Tan, K Vints, K De Cremer, A Braem,
N Verstraeten, J Michiels, J Vleugels, B Cammue, K Thevissen
PS01.23
Specific quantification of Pseudomonas aeruginosa in a multispecies
biofilm with PMA-qPCR
Sarah Tavernier, (Belgium), T Coenye
PS01.24
Extracellular matrix-mediated interspecies interactions mediate
co-aggregation and resistance during dual-species biofilm formation
Liang Yang, (Singapore), SC Chew, JKH Yam
PS01.25
The cariogenic potential of Streptococcus mutans and Candida albicans
in an in vitro dual-species biofilm model
Hubertine ME Willems, (The Netherlands), K Kos, BP Krom
PS01.26
A mouse model for chronic fungal and poly-microbial osteomyelitis
Cecilie Christiansen, (Denmark), T Bjarnsholt, M Shirtliff, N Høiby,
S Eickhardt
PS02
MIXED SPECIES FUNGAL & BACTERIAL BIOFILM COMMUNITIES (CONT.)
PS01.27
Assessing synergistic interaction between Gardnerella vaginalis and
other urogenital pathogens
Joana Castro, (Portugal), D Machado, N Cerca
PS01.28
Hemin stimulates growth, aggregation and formation of dual species
Bifidobacterium dentium – Streptococcus mutans biofilm
Michel Hoogenkamp, (The Netherlands), M Willems, H de Soet, W Crielaard
PS01.29
Poly-microbial biofilms – prevalence and importance in infections
Trine Rolighed Thomsen, (Denmark), Y Xu, L Larsen, J Lorenzen
PS01.30
Effect of bacteria on the behavior of biofilms of ligninolytic fungi
Hana Válková, (Czech Republic), C Novotný
-22-
PS01.32
Optimization of Candida parapsilosis biofilm preparation protocols for scanning electron
microscopy
Filip Růžička, (Czech Republic), K Hrubanova, J Nebesarova, V Krzyzanek
PS03
TREATMENT OF BIOFILM INFECTIONS
PS01.33
Increased bactericidal activity of colistin on Pseudomonas aeruginosa
biofilms in anaerobic conditions
Mette Kolpen, (Denmark), CF Appeldorff, S Brandt, N Mousavi, KN Kragh,
T Bjarnsholt, O Ciofu, N Høiby, PØ Jensen
PS01.34
Biofilm of endotracheal tubes from patients with MRSA pneumonia
treated with linezolid: preliminary results
Laia Fernandez-Barat, (Spain), A Motos, M Ferrer, L Guerrero, F Álvarez-Lerma, L Viña,
C Chiurazzi, D Soy, G Li Bassi, J Vila, A Torres
PS01.35
Polydopamine-mediated immobilization of PALM and DNAse to create
an anti-adhesive and antimicrobial bi-functional coating
Diana Alves, (Portugal), P Jorge, MO Pereira
PS01.36
Low dose nitric oxide as targeted anti-biofilm adjunctive therapy to treat chronic
Pseudo monas aeruginosa infection in cystic fibrosis
Jeremy S Webb, (UK), K Cathie, RP Howlin, L Hall-Stoodley, V Cornelius,
C Duignan, R Allan, BO Fernandez, N Barraud, K Bruce, J Jefferies, M Kelso,
S Kjelleberg, S Rice, G Rogers, S Pink, C Smith, P Sukhtankar, R Salib,
J Legg, M Carroll, T Daniels, M Feelisch, P Stoodley, SC Clarke, G Connett, SN Faust
PS03
TREATMENT OF BIOFILM INFECTIONS (CONT.)
PS01.37
Identifying compounds that increase antibiotic activity on biofilms
Tom Coenye, (Belgium), F Van den Driessche, G Brackman, P Rigole
PS01.38
Nonmevalonate pathway for isoprenoid biosynthesis as novel target for antibacterial
therapy against the Burkholderia cepacia complex
Tom Coenye, (Belgium), A Everaert, R Chofor, S Van Calenbergh
PS01.39
Influence of intervallic shear stress application on oral biofilms
Pune Nina Tawakoli, (Switzerland), Bl Sauer, K Becker, T Attin
PS01.40
The potential of novel gentamicin-black seed oil (GBSO) fusion
emulsion in treating biofilm infection
Mohd Affendi Mohd Shafri, (Malaysia), KI Yaakob, FA Yusof,
IA Shahdan, N Mohd Yusof, F Mohamed
PS01.41
Short-term effect of the maintenance therapy on the phenotypic
characteristics of P. aeruginosa populations from chronically
infected CF patients
Laia Fernandez-Barat, (Spain), O Ciofu, U Johansen, T Wassermann,
T Pressler, A Motos, A Torres, N Hoiby
PS01.42
Extracellular DNA in biofilms associated with chronic otitis media
with effusion
Ali Mostafa Wayes, (UK), NS Jakubovics, MR ElBadawey, JG Burgess
PS01.43
Anti-biofilm peptide combinations in the eradication of pre-established biofilms of
Pseudomonas aeruginosa and Staphylococcus aureus
Paula Jorge, (Portugal), D Grzywacz, W Kamysz, A Lourenço, MO Pereira
-23-
POSTER SESSION I
13:00 – 15:00
Determination of gelatinase (gelE) expression levels, a virulence gene of Enterococcus
faecalis, in planktonic form, monomicrobial and dual species biofilms
Meral Sagiroglu, (Turkey), D Kart, A Kalkanci, S Kustimur
WEDNESDAY, 24 JUNE 2015
13:00 – 15:00
PS01.31
POSTER SESSION I
WEDNESDAY, 24 JUNE 2015
13:00 – 15:00
13:00 – 15:00
PS01.44
A novel antioxidant polymer active against Staphylococcus epidermidis
Iolanda Francolini, (Italy), F Crisante, V Taresco, G Donelli, C Vuotto,
A Martinelli, L D’Ilario, L Pietrelli, A Piozzi
PS01.45
Anti-Candida biofilm activities of two Cameroonian plant extracts
Elisabeth Zeuko´o Menkem, (Cameroon), V Cepas Lopez, S Soto,
F Fekam Boyom
PS01.46
Efficacy of sequential use of inhaled antibiotics in the treatment of Pseudomonas
aeruginosa in an in vitro biofilm model
Estrella Rojo-Molinero, (Spain), R Rubio, MD Macià, B Moyà,
JL Pérez, R Cantón, A Oliver
PS01.47
Comparison of random amplified polymorphic DNA typing and phenotypic characterization
of Pseudomonas aeruginosa strains isolated from patients hospitalized on Intensive
Care Units
Martina Vaněrková, (Czech Republic), V Holá, E Němcová, B Mališová,
T Freiberger
PS01.48
Effectiveness of antimicrobial agents used for an elimination of the oral biofilm vs. PDI
with methylene blue on Streptococcus mutans biofilm
Kornelia Legenová, (Slovakia), M Kovalčíková, H Bujdáková
PS03
TREATMENT OF BIOFILM INFECTIONS (CONT.)
PS01.49
Role of TanReQing injection in disrupting Staphylococcus aureus
biofilm through inhibiting cell division
Yi Wang, (China), C Liu, W Yang, S Liu
PS01.50
Antibiotic release from absorbable calcium sulfate beads for the prevention and
treatment of biofilm formation in orthopaedic prosthetic infection
Paul Stoodley, (USA), C Knecht, S Diamond, N Farrar, C Peters,
M Swearingen, JJ Cooper, SS Aiken, J Granger, RP Howlin
PS04
SOCIAL INTERACTIONS IN BIOFILMS
PS01.51
Cooperative interactions in a four species biofilm community
Mette Burmølle, (Denmark), D Ren, JS Madsen, J Herschend,
L Madsen, SJ Sørensen
PS01.52
Selective signaling inhibition by extracellular polysaccharide alginate
Jiayue Yang, (Japan), M Toyofuku, R Sakai, K Tateda, N Nomura
PS01.53
Staphylocoagulase, an exploitable intra- and inter-specific public good
Urvish Trivedi, (Denmark), JS Madsen, M Burmølle, SJ Sørensen
PS01.54
P. aeruginosa PAO1 provides grazing resistance to sensitive strains in a multispecies biofilm
Henriette Lyng Røder, (Denmark), SH Chan, KWKn Lee, D McDougald,
M Burmølle, SJ Sørensen, SA Rice
PS01.55
Small RNAs expressed in Burkholderia cenocepacia biofilms
Sanne Kiekens, (Belgium), A Sass, T Coenye
PS01.56
Differential effects of biofilm supernatant of Pseudomonas aeruginosa
and Serratia marcescens on biofilm formation of clinical Staphylococcus epidermidis
isolates
An Hotterbeekx, (Belgium), S De Backer, P Moons, C Lammens, M Ieven,
E Vandenbroeck, P Jorens, S Kumar-Singh, H Goossens, S Malhotra-Kumar
PS01.57
Effect of farnesol on the ERG gene expression in Candida biofilm
Stanislava Dižová, (Slovak Republic), H Bujdáková
PS01.58
Structural characterization of a synergistic multi-species biofilm consortium
Wenzheng Liu, (Denmark), J Herschend, SJ Sørensen, M Burmølle
-24-
THURSDAY, 25 JUNE 2015
POSTER SESSION II
Time
10:30 – 12:30
13:00 – 15:00
13:00 – 15:00
13:00 – 15:00
S05.3
HOST RESPONSES TO BIOFILMS
PS02.01
Staphylococcus epidermidis response to human blood and its cellular and
soluble components
Nuno Cerca, (Portugal), A França, M Vilanova, G Pier
PS02.02
Dormancy within Staphylococcus epidermidis biofilms: an
immunoproteomic characterization
Virginia Carvalhais, (Portugal), N Cerca, M Vilanova, R Vitorino
PS02.03
Host defence against bacterial biofilms: activation of defence-relevant functions
by bacterial heat shock proteins
Ulrike Dapunt, (Germany), S Maurer, P Fouchard, E Meyle, GM Hänsch
PS02.04
Extracellular dATP/ATP induces eDNA release and biofilm formation in Candida albicans
Suma Pemmaraju, (India), P Pruthi, R Prasad, V Pruthi
PS06
BIOMECHANICS IN BIOFILM AND INFECTIONS
PS02.05
Application of AFM for morphological, mechanical and adhesive characterisation
of microbial biofilms
Dimitar Stamov, (Germany), T Müller, H Haschke, T Jähnke
PS02.06
Developing a materials-based understanding of Bacillus subtilis
biofilm mechanics
James Wilking, (USA)
PS02.07
An internal pushing force within a biofilm
Eric Raspaud, (France), C Douarche, J-M Allain
PS02.08
Fluid forces to disrupt oral biofilms
Amir Rmaile, (The Netherlands), B Gottenbos, M de Jager, D Carugo, L Capretto,
M Aspiras, M Ward, P Stoodley
PS02.09
Bridge bacteria induced aggregation of Listeria monocytogenes
Xiangwu Nou, (USA), G Ailing, J Mowery, A Nagy, G Bauchan
PS07
RESISTANCE IN BIOFILMS & NOVEL APPROACHES TO TREATMENT
PS02.09
Biological evaluation of an in vivo-potent dual target PQS-Quorum
Sensing inhibitor that hinders biofilm formation
Antonio Gurdjieff Gomes de Mello Martins, (Germany), A Thomann,
C Brengel, E Weidel, A Plaza, C Börger, M Empting, RW Hartmann
PS02.10
Interplay between NO and H2S gasotransmitters in the bacterial
biofilm production and dispersion
Daria Streltsova, (Russian Federation), S Vasilieva, M Petrishcheva
PS02.11
Type 1 fimbriae of multidrug resistant E. coli ST131: regulation,
function and inhibition using novel antivirulence therapeutics
Makrina Totsika, (Australia), S Sarkar, M Schembri
PS02.12
Ability of Candida albicans to induce Staphylococcus aureus biofilm
tolerance to vancomycin
Sona Kucharikova, (Belgium), E Kong, P Van Dijck, MA Jabra-Rizk
PS07
RESISTANCE IN BIOFILMS & NOVEL APPROACHES TO TREATMENT (CONT.)
PS02.13
Antibiotic-Nigella Sativa Fusion (ANF) as a novel approach intended
for the treatment against bacteria forming biofilm in osteomyelitis
Farahidah Mohamed, (Malaysia), MA Mohd Shafri, N Mohd Yusof,
AF Harun, KI Yaakob, FA Yusof, IA Shahdan
-25-
POSTER SESSION II
THURSDAY, 25 JUNE 2015
13:00 – 15:00
PS02.14
Identification of new anti-infective molecules from marine bacteria
active against the biofilm of S. Aureus and P. aeruginosa
Ermenegilda Parrilli, (Italy), R Papa, M Tilotta, F Sannino,
ML Tutino, M Artini, L Selan
PS02.15
Inhibiting effects of fructanase on the competence-stimulating
peptide-dependent genetic transformation and biofilm formation
by Streptococcus mutans
Yusuke Suzuki, (Japan), T Arai, N Ogura, T Kondoh, H Senpuku
PS02.16
The anti-biofilm activity secreted by the Antarctic bacterium Pseudoalteromonas
haloplanktis TAB23
Ermenegilda Parrilli, (Italy), F Sannino, A Ricciardelli, R Papa,
M Tilotta, M Artini, L Selan, ML Tutino
PS02.17
The antifungal caspofungin (CAS) increases moxifloxacin (MXF)
activity against Staphylococcus aureus biofilms in vitro or in a mice subcutaneous model
Wafi Siala, (Belgium), S Kucharíková, PM Tulkens, P Van Dijck,
F Van Bambeke
PS02.18
Anti-biofilm activity of Pseudoalteromonas haloplanktis
against Staphylococcus epidermidis: evidences of a signal
molecule involvement
Maria Michela Corsaro, (Italy), E Parrilli, R Papa, M Tilotta,
A Casillo, F Sannino, M Artini, L Selan, ML Tutino
PS02.19
Antimicrobial and antibiofilm activities of Cellobiose Dehydrogenase functionalized
urinary catheters
Gibson Stephen Nyanhongo, (Austria), B Thallinger, M Brandauer,
A Schlick, P Burger, R Ludwig, C Sygmund
PS02.20
TBBQ: mechanism of biofilm eradication and potential for clinical use
Nicola Ooi, (UK), A O’Neill
PS02.21
Search for potentiators of miconazole resulting in increased activity
against Candida albicans biofilms
Kaat De Cremer, (Belgium), TL Cools, I Staes, K De Brucker,
E Lanckacker, F Van Den Driessche, T Coenye, P Cos, BPA Cammue,
K Thevissen
PS02.22
Relationship between the biofilm formation by Pseudomonas aeruginosa, antimicrobial
resistance and its efflux pumps overexpression
Gertrudis Horna Quintana, (Spain), J Ruiz Blazquez
PS07
RESISTANCE IN BIOFILMS & NOVEL APPROACHES TO TREATMENT (CONT.)
PS02.23
Cystic fibrosis bacteria under variable oxygen tensions: biofilm
formation ability and resilience to acute antibiotherapy
Susana Lopes, (Portugal), A Magalhães, M Pereira
PS02.24
Disruption of Escherichia coli biofilms by rhamnolipids: effect of
the medium, temperature and concentration
Marcia Nitschke, (Brazil), SS Silva
PS02.25
Synthetic chalcone derivatives as agents to control P. aeruginosa biofilms
Marcia Nitschke, (Brazil), MD Bocelli, IL Matos, ALM Porto
PS02.26
Impact of superhydrophobic coating on microbial adhesion and
biofilm growth
Eva Travnickova, (Czech Republic), L Blaha, M Klima,
M Gasic, S Ivanova
PS02.27
The role of biocide adaptation on biofilm formation and susceptibility
Joana Malheiro, (Portugal), IB Gomes, F Mergulhão, L Melo, M Simões
-26-
PS02.29
Fast development of ciprofloxacin resistance in P. aeruginosa and
S. epidermidis biofilms exposed to biofilm sub-inhibitory concentrations
of ciprofloxacin in an evolution experiment
Oana Ciofu, (Denmark), K Ivanyshyn, T Wassermann, N Høiby
PS02.30
Physiological glucose concentrations trigger LPS production and
virulence in a Acinetobacter baumannii
Francesca Longo, (Italy), E Rossi, C Peano, L Barbagallo,
S Jaillon, A Petrelli, C Garlanda, P Landini
PS02.31
Effects of tobramycin and colistin antibiotic treatment against
planktonic P. aeruginosa during various growth phases
Blaine Fritz, (Denmark), K Kragh, T Bjarnsholt
PS02.32
Photodynamic therapy of bacterial biofilms with the new cationic photosensitizer
Irina Tiganova, (Russia), T Stepanova, E Tolordava, G Meerovich,
Y Koloskova, S Brusov, M Grin, A Mironov, Y Romanova
PS02.33
Fungal infectivities of implanted catheters dues to Candida sp. biofilms formation and
resistance
Sidi Mohammed Lahbib Seddiki, (Algeria), Z Boucherit-Otmani, K Boucherit, D Kunkel
PS07
RESISTANCE IN BIOFILMS & NOVEL APPROACHES TO TREATMENT (CONT.)
PS02.34
A novel ultra-dense polyethylene glycol coating for medical implants challenge
Staphylococcus epidermidis biofilms in vitro
Sandra Maria Skovdal, (Denmark), N Jørgensen, R Ogaki, G Zeng,
RL Meyer, E Petersen
PS02.35
Vancomycin conjugated magnetic nanoparticles against biofilms
on prosthetic joint infection
Yen-Chun Chen, (Taiwan), C-N Chang, M-R Jhang, M-C Lin, M-D Yang,
S-I Huang, W-Y Hsieh, M-J Tung, S-J Wang, H-W Tseng
PS08
MODELLING OF BIOFILMS & REGULATION OF BIOFILM DEVELOPMENT
PS02.36
Dangers of sticking together - biofilm aggregation in liquid batch cultures
Kasper Nørskov Kragh, (Denmark), M Alhede, M Alhede, PØ Jensen,
T Bjarnsholt
PS02.37
New insights in Legionella pneumophila biofilm regulation by c-di-GMP signaling
Sophie Pecastaings, (France), J Allombert, P Doublet, C Roques, A Vianney
PS02.38
Hydrophobic segments in exopolysaccharides of Burkholderia biofilms
Paola Cescutti, (Italy), C Buriola, A Sveronis, A Delneri, M Fazli,
T Tolker-Nielsen, R Rizzo
PS02.39
Mineral scaffolds enable the morphogenesis of bacterial biofilms
Yaara Oppenheimer-Shaanan, (Israel), O Sibony-Nevo, Z Bloom-Ackermann, R Suissa,
N Steinberg, V Brumfeld, E Kartvelishvily, I Kolodkin-Gal
PS02.40
Mapping of transcription start sites in Burkholderia cenocepacia
Tom Coenye, (Belgium), A Sass, S Kiekens
PS02.41
Development of in-vitro test system for nebulized antimicrobial
treatment efficacy using Pseudomonas aeruginosa biofilms
Laura Boge, (Germany), J Pankalla, M Müller, A Braun,
G Pohlmann, S Wronski
-27-
POSTER SESSION II
13:00 – 15:00
Cinnamic acid modifies bacterial surface and induces biofilm removal
Joana Malheiro, (Portugal), IB Gomes, AP Borges, M Simões
THURSDAY, 25 JUNE 2015
13:00 – 15:00
PS02.28
POSTER SESSION II
THURSDAY, 25 JUNE 2015
13:00 – 15:00
13:00 – 15:00
PS02.42
Stage-dependent changes in matrix composition of biofilms formed by methicillin-resistant
Staphylococcus aureus (MRSA) USA300
Sarah De Backer, (Belgium), C Vanmarsenille, J-P Hernalsteens,
BB Xavier, H De Greve, H Goossens, M-K Surbhi
PS02.43
Optimization of in vitro model for growth of Gardnerella vaginalis biofilm
Daniela Machado, (Portugal), A Palmeira-de-Oliveira, N Cerca
PS02.44
Low-dose amoxicillin stimulates MRSA intercellular adhesion and
surface attachment
Jeffrey Kaplan, (USA), K Mlynek, A Shimkevitch, J Farmer, J Endres,
K Bayles
PS02.45
Gene expression during biofilm formation in clinical strains of
Staphylococcus epidermidis associated with neonatal infections
Monika Grzebyk, (Poland), M Brzychczy-Woch, A Piotrowska, PB Heczko
PS02.46
Biofilm formation in vitro by Staphylococcus aureus isolated from nasopharynx
and adenoids in children after adenoidectomy - phenotypic and genotypic analysis
Anna Malm, (Poland), A Grzegorczyk, I Korona-Glowniak, A Niedzielski
PS02.47
Effect of EPS in Staphylococcus epidermidis biofilm mediated infections using the
nematode model Caenorhabditis elegans
Birthe V Kjellerup, (USA), C Adams, A Houston, S Edwards
PS08
MODELLING OF BIOFILMS & REGULATION OF BIOFILM DEVELOPMENT (CONT.)
PS02.48
Fluoride effect on genotypic diversity and acidogenicity of
Streptococcus mutans isolated from in situ oral biofilms
Glauber Vale, (Brazil), R Arthur, A Del Bel Cury, J Cury, C Tabchoury
PS02.49
Development of an in vitro wound infection model to study interactions between dermal
cells and biofilm bacteria and new treatment strategies
Maria Werthen, (Sweden), E Gerner, A Parseus
PS02.50
Detailed analysis of c-di-GMP mediated regulation of csgD expression
in Salmonella typhimurium
Irfan Ahmad, (Sweden), T Beske, A Cimdins, U Romling
PS09
PROBIOTICS AND NEW NATURAL & SYNTHETIC
ANTI-BIOFILM COMPOUNDS
PS02.51
Inhibitory effect of flavonolignans on microbial biofilm formation
Vladimir Vimberg, (Czech Republic), M Kuzma, E Stodùlková,
P Novák, L Bednárová, M Šulc, G Balikova Novotna, R Gažák
PS02.52
Modulation of Staphylococcus aureus biofilm by metabolites from Lactobacillus mucosae
Dobroslava Bujnakova, (Slovak Republic), E Strakova, V Kmet
PS02.53
Inhibitory effect of novel 1,2,4-triazole-cirofloxacin hybrids on
Haemophilus parainfluenzae biofilm in vitro
Anna Malm, (Poland), U Kosikowska, S Andrzejczuk, T Plech
PS02.54
Lichens: promising source of inhibitors of Candida biofilms
Marion Girardot, (France), L Teulière, M Millot, L Mambu, C Imbert
PS02.55
Synergistic activity of biosurfactant AC7 and farnesol on
Candida albicans biofilm formation on medical silicone elastomer
Francesco Tessarolo, (Italy), C Ceresa, I Caola, D Maniglio,
F Piccoli, G Nollo, L Fracchia
PS02.56
Search of strain pool compositions of interacting microorganisms stabilizing human
biotope when known panels of antibiotics will be used in therapy
Vladimir Lakhtin, (Russian Federation), M Lakhtin, S Afanasiev, A Bajrakova, V Aleshkin
-28-
13:00 – 15:00
PS10
HORIZONTAL GENE TRANSFER, EVOLUTION & HETEROGENEITY
IN MICROBIAL BIOFILMS
PS02.58
Plasmid stability in biofilm vs. planktonic environments
Ida Thalsø-Madsen, (Denmark), JS Madsen, H Røder,
U Trivedi, L Riber, M Burmølle, SJ Sørensen
PS02.59
c-di-GMP related genes are common on plasmids - a comparative analysis
Jonas Stenløkke Madsen, (Denmark), O Hylling, S Jacquiod,
G Vestergaard, M Burmølle, L Riber, LH Hansen, SJ Sørensen
PS02.60
Helicobacter pylori Outer Membrane Vesicles (OMVs) as
DNA “shuttles” and structural components of biofilm EPS
Rossella Grande, (Italy), MC Di Marcantonio, I Robuffo,
A Pompilio, R Primavera, M Di Francesco, E Di Bartolomeo,
G Di Bonaventura, P Stoodley, L Hall-Stoodley, G Mincione
PS02.61
Bacterial fortresses: secreted matrix structure determines invasion
resistance in bacterial biofilms
Carey Nadell, (Germany), K Drecher, N Wingreen, B Bassler
PS02.62
Promoted flocculation of Escherichia coli cells in association
with outer membrane vesicle production
Yoshihiro Ojima, (Japan), MH Nguyen, R Yajima, M Taya
PS11
ENVIRONMENTAL BIOFILMS AND HUMAN HEALTH
PS02.63
Microbiome changing inside dental pipes: a case study
Damien Costa, (France), A Mercier, J Lesobre,
V Delafont, C Imbert
PS02.64
Activity and diversity of biofilm bacteria involved in enhanced
bioremediation of contaminated sediment
Birthe V Kjellerup, (USA), S Edwards, F Akbari
PS02.65
Effect of sodium hypochlorite on bacteria isolated from drinking water
Inês B Gomes, (Portugal), M Lemos, J Malheiro, L Simões, M Simões
PS02.66
Contaminating bacteria and pathogens on three minimally
processed herbs: CFUs, biofilm formation capacity, and antibiotic resistances
Maria-Theresia Gekenidis, (Switzerland), D Gossin, MNP Remus-Emsermann, D Drissner
PS02.67
Biofilm formation potential of S. aureus dairy isolates representing
different genotypes
Elowine Thiran, (Switzerland), HU Graber, J Hummerjohann
PS02.68
Biocides on the disinfection of silicone and stainless steel surfaces
Inês B Gomes, (Portugal), J Malheiro, AP Borges, F Mergulhão, M Simões
PS11
ENVIRONMENTAL BIOFILMS AND HUMAN HEALTH (CONT.)
PS02.69
Surface protein profiles of Staphylococcus aureus biofilm cells
Zoran Jaglic, (Czech Republic), L Cincarova, D Cervinkova
PS02.70
Utilization of mixed biofimls in degradation of synthetic dye
Pavlina Slosarcikova, (Czech Republic), C Novotný
-29-
POSTER SESSION II
13:00 – 15:00
The effect of selected antifungals on the biofilm formation by C. albicans strains isolated
from the oral cavity of adults
Jolanta Szymanska, (Poland), A Malm, A Wójtowicz
THURSDAY, 25 JUNE 2015
13:00 – 15:00
PS02.57
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The goal of ESGB is to promote and disseminate studies and knowledge about methods and results of biofilm studies
with relevance for infections in humans. The ultimate goal is to improve diagnostics and the results of prophylaxis and
therapy of biofilm infections in humans.
Please join us at: https://www.escmid.org/research_projects/study_groups/biofilms/
-30-
Notes
Organised and supported by
European Society of Clinical Microbiology and Infectious Diseases (ESCMID)