Download rs2 - CytoMaize.ORG

“rough sheath2 (rs2)”
By Charlene M. Kormondy
& Sarah R. Higbee-Tindell
Student Projects on Maize Mutants
Middle School Gifted Science Class
Fall 2004 Mentorship Program
Maize Genetics with Biologist H. Bass
School of Arts and Sciences (SAS) Charter School
Leon County School System, Tallahassee, FL
Credits
Science Teacher:
FSU Mentor:
Assistants:
Additional thanks to:
Joan Crow, SAS Science Teacher
Hank W. Bass, Assistant Professor of Biological Science, FSU
Bobbye Hill Biological Research Scientist, Bass lab, FSU
Debbie M. Figueroa, Graduate Student, Bass lab, FSU
Brian C. Ring, Post-doctoral Research Associate, Bass lab, FSU
Julia Winter, SAS Math teacher
Amber N. Brown, Graduate Student, Bass lab, FSU
Links
The SAS school web page
The SAS Mentorship Fall ‘04 page
The Maize-10-Maize project
http://www.artsandsciences.leon.k12.fl.us/main/
http://www.cytomaize.org/outreach/sas/
http://www.cytomaize.org/outreach/
Hank Bass laboratory home page
email contact: [email protected]
http://bio.fsu.edu/bass/
rough sheath2 (rs2)
By Charlene Kormondy and
Sarah Higbee-Tindell
rough sheath2 (rs2), CMK, SRH, 1-13-05
Table of Contents
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Information
What’s wrong with the mutant
DNA sequence
DNA sequence
explanation
• References
Information
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Bass lab family number: 1558
The rs2 gene has been cloned
The gene is on chromosome 1
The mode of inheritance is recessive
What is wrong with the mutant?
The rs2 mutant has warty distorted leaf blade & sheath.
Normal
Plant
Mutant
Plant
DNA sequence of rs2
Zea mays rough sheath2 (rs2) cDNA gene sequence
>gi|4583430|gb|AF126489.1|AF126489
1 CGCGCATTAT AAGGTGTGGT GGTGAGGGAT GGGCGATGGC CTTTGCAGAT GAAGGAGCGA 61 CAGCGCTGGC GGCCTGAGGA AGACGCCGTC CTGCGCGCCT ACGTCCGGCA GTACGGCCCC 121 CGCGAGTGGC ACCTGGTGTC GCAGCGCATG AACGTGGCCC TCGACCGCGA CGCCAAGTCG 181 TGCCTGGAGC GCTGGAAGAA CTACCTCCGC CCCGGGATCA AGAAGGGCTC GCTCACCGAG 241 GAGGAGCAGC GCCTGGTGAT CCGCCTGCAG GCCAAGCACG GCAACAAGTG GAAGAAGATC 301 GCCGCCGAGG TGCCCGGGCG CACGGCCAAG CGCCTCGGCA AGTGGTGGGA GGTGTTCAAG 361 GAGAAGCAGC AGCGGGAGCT GAGGGACAGC CGCCGCCCGC CGCCGGAGCC TAGCCCCGAC 421 GAGCGGGGCA GGTACGAGTG GCTGCTCGAG AACTTCGCGG AGAAGCTCGT CGGAGAGAGG 481 CCACAGCAGG CTGCCGCTGC GCCGTCGCCG CTGCTCATGG CCGCGCCCGT GCTGCCGCCC 541 TGGCTGTCGT CCAACGCCGG GCCGGCCGCC GCCGCCGCCG CCGCCGTCGC GCACCCGCCG 601 CCCAGACCGC CGTCGCCGTC CGTGACGCTC AGCCTCGCGT CCGCCGCGGT GGCGCCGGGC 661 CCGCCCGCGC CCGCGCCATG GATGCCGGAT CGCGCCGCGG CCGACGCGGC GCCGTACGGG 721 TTCCCGAGCC CGTCGCAGCA CGGCGGCGCC GCGCCCCCGG GCATGGCGGT CGTGGACGGG 781 CAGGCGCTGG CGGAGCTCGC CGAGTGCTGC CGCGAGCTGG AGGAGGGGCG GCGCGCGTGG 841 GCGGCGCACC GGCGGGAGGC GGCGTGGCGG CTGAAGCGCG TGGAGCAGCA GCTGGAGATG 901 GAGCGCGAGA TGCGGCGGCG CGAGGTGTGG GAGGAGTTCG AGGCCAAGAT GCGCACCATG 961 CGGCTGGAGC AGGCGGCCGC CGCGGAGCGC GTGGAGCGGG ACCACCGGGA GAAGGTGGCC 1021 GAGCTCCGCC GCGACGCGCA GGTCAAGGAG GAGAAGATGG CCGAGCAGTG GGCCGCCAAG 1081 CACGCGCGCG TCGCCAAGTT CGTCGAGCAG ATGGGGGGGT GCTCCCGTTC ATGGTCCTCT 1141 GCCACCGACA TGAACTGTTG AACTGGACAA ACGAGCCGCT GACCACACCG TCGCTAGCTA 1201 TCGCGTGCTA CGTGCTCCGG TTCACGGCTT TCTTGCATTC TGGGACGCGA CTTGTAAACG 1261 CCATGGATTC CCTTGCTCAC GTTCACTTCC GCTTTCTTTT TTTCTTTTCT TTTTATTTAC 1321 AACTGTTGTT ACGGTCGGTT CAATGCTTCT CTCTCTATCT ACTAGTAGTT GATCAGAGTC 1381 AAGAAATGTA ATGCTGCCGT TACTGTCGTA GTTTCATCCT DNA sequence Explanation
DNA = DeoxyriboNucleic Acid
A = Adenine
G = Guanine
C = Cytosine
T = Thymine
A & T go together
G & C go together
First strand = GCTA
Second strand =CGAT
Reference
Hadjinov, M.I. (1937)
Genes of “Rough Sheath” in maize.
Bull Appl Bot Gen Plant Breed Ser II 7:247-258.
Thanks to:
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Dr. Hank Bass
Ms. Debbie Figueroa, graduate student
Ms. Bobbye Hill, biologist
Dr. Brian Ring, post-doctoral research
associate
• And of course, the wonderful Mrs. Crow
Field placard
Gene Name:
rough sheath2, rs2
Locus:
Chromosome # 1
Placard By:
Charlene Kormondy &
Sarah Higbee-Tindell
The School of Arts and Sciences
Middle School Mentorship
THE END