Download Formulation for Dulbecco`s Modified Eagle`s Medium (DMEM) ATCC

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Formulation for Dulbecco’s Modified Eagle’s
Medium (DMEM) ATCC® 30-2002
Inorganic Salts (g/liter)
CaCl2 (anhydrous)
Fe(NO3)3·9H2O
MgSO4 (anhydrous)
KCl
NaHCO3
NaCl
NaH2PO4·H2O
Vitamins (g/liter)
0.20000
0.00010
0.09770
0.40000
1.50000
6.40000
0.12500
Amino Acids (g/liter)
L-Arginine·HCl
L-Cystine·2HCl
L-Glutamine
Glycine
L-Histidine·HCl·H2O
L-Isoleucine
L-Leucine
L-Lysine·HCl
L-Methionine
L-Phenylalanine
L-Serine
L-Threonine
L-Tryptophan
L-Tyrosine·2Na·2H2O
L-Valine
0.08400
0.06260
0.58400
0.03000
0.04200
0.10500
0.10500
0.14600
0.03000
0.06600
0.04200
0.09500
0.01600
0.10379
0.09400
Choline Chloride
Folic Acid
myo-Inositol
Nicotinamide
D-Pantothenic Acid
(hemicalcium)
Pyridoxine·HCl
Riboflavin
Thiamine·HCl
0.00400
0.00400
0.00720
0.00400
0.00400
0.00400
0.00040
0.00400
Other (g/liter)
D-Glucose
Phenol Red, Sodium Salt
Sodium Pyruvate
ATCC (American Type Culture Collection)
P.O. Box 1549
Manassas, VA 20108 USA
www.atcc.org
4.50000
0.01500
0.11000
800-638-6597 or 703-365-2700
Fax: 703-365-2750
E-mail: [email protected]
Or contact your local distributor
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