Download Alignment Table

Implementing Vision and Change Using Concept-Driven Teaching Strategies
NSF RCN-UBE Grant #0957205
Designing Scientific Teaching Tools for BMB Education
UC Santa Barbara
February 8, 2014
BMB Alignment Table Template
Designed by:
Christoffsen, Rolf
Nogaj, Luiza
Clark, Dan
Morales-Díaz, Heidi
Selected Aspect of BMB:
A2, Mutations at the molecular level
Keywords:
Mutation, gene expression, gene splicing, Progeria, genetic dominance
Initial Overall Learning Goal: (junior/senior level teaching)
Students should understand and predict how a mutation can change the amino acid sequence of
a gene product and how it defines a phenotype.
Initial Specific Learning Objective:
Students should be able to explain how changes in nucleotide, amino acid sequence, transcript
splicing can change gene expression and protein structure and function.
Overall Learning Goal: Insert refined goal
Specific
Learning Objective
Bloom level 1-2:
Students should compare and
contrast a mutation in an exon
or an intron that affect a protein
sequence
Bloom level 3-4:
Students should be able to
analyze a data set to identify
disease characteristics (PCR
and Western blot)
Bloom level 5-6:
Students should be able to
analyze a novel disease to
predict its outcome and
formulate additional scientific
approaches to get more
information of the disease
Assessment:
Specific
Learning Assessment
Specific
Learning Strategy
Written exam #1:
Describe the molecular
mechanisms of mutations
and how they affect protein
sequence
Written exam #2: Data set
analysis in a case study of a
Progeria like disease
(diagram)
Final Assessment, take
home test?:
Design an evaluation to
better identify disease X and
create an approach to treat
the disease (how can you
treat this disease)
Diagram of nucleotide and
amino acid sequences in
health and disease
Use PCR and western blot
data analysis tools to
evaluate a patient
Case study on Progeria
(Hutchinson–Gilford
progeria syndrome) as a
disease model for a splice
mutation in lamin-A using
real data.
Students will be assessed on their approach to different challenges at increasing levels.
Objective Questions
1
1-3
Beginning
1
none correct
2
1-3
none correct
3
1-3
Developing Accomplished Exemplary
2
3
4
1 correct
2 correct
3 correct
PCR only
Wb only
Wb+PCR
Beginning: no logical hypothesis or evaluation Developing: logical
hypothesis, no good evaluation Accomplished: Conjure a treatment that is
not feasible Exemplary: Connection with real data/show precedence
Strategy:
Design your classroom or laboratory strategy here, and be sure to include the time allotted.
1:30hr class
Pre-class reading required, 1hr
Present case study 15minutes
Group discussion 45 minutes
Discussion and answers 20-30 minutes
Progeria:
Rare genetic disorder resulting in premature aging
Due to a toxic accumulation of a truncated form of lamin-A/C (progerin) which is generated by a
silent or missense mutation that causes activation of a cryptic splice donor site, resulting in
deletion of a cleavage site. This renders the mutant progerin permanently farnesylated and
cannot incorporate into the nuclear lamina (toxic accumulation).