Download CV Fabrizio Salomone - Scuola Normale Superiore

PERSONAL INFORMATION
Fabrizio Salomone, PhD candidate
Sex Male | Date of birth 26/09/1984 | Nationality Italian
| Languages Italian (mother tongue), English (Mastery),
French (Intermediate).
Basic knowledge of Chinese (HSK 1 level certificate 2011)
PROFESSIONAL EXPERIENCES
03/2013 to 10/2013
Research stage at Institute Gustave Roussy
Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203. Villejuif (Fr).
Reference: Directeur de Recherche Dr. Lluis Mir.
[email protected]
Équipe de Chimie et Biochimie Théoriques, UMR 7565. Nancy (Fr).
Reference: Directeur de Recherche Dr. Mounir Tarek.
[email protected]
▪ Project: Integration of electric nanopulses with endosomolytic cell-penetrating peptides (CPPs) to
improve cytosolic drug delivery. In vitro and In silico characterization.
▪ Applied techniques: Electrogenetherapy, GUVs manufacturing, Cell culturing, Confocal microscopy,
Flow cytometry, All-atoms Molecular Dynamics Simulations (NAMD, VMD, Packmol).
10/2009 to present
PhD project development at Scuola Normale Superiore di Pisa
NEST Laboratory. Pisa (It).
Reference: Prof. Fabio Beltram.
[email protected]
▪ Project: Improving CPPs escape from the endosomal entrapment by the conjugation of Tat peptide
with antimicrobial peptides for efficient drug delivery.
▪ Applied techniques: Immortalized and primary cell culturing, WST-8 assay, Gene transfection,
Luciferase assay, Confocal and Two photon microscopy, Fluorescence techniques (FCS, FRET,
FRAP, FLIM), CD spectroscopy, PCR, Plasmid construction and amplification, Electrophoresis,
Recombinant protein expression, Western blotting, Labelling reactions, Patch clamp.
▪ See annexes for papers and posters.
09/2008 to 09/2009
Master stage at University of Catania
Department of Chemistry, Faculty of Science. Catania (It).
Reference: Prof. Enrico Rizzarelli
[email protected]
▪ Project: Characterization of metals effect on the interaction between Insulin degrading enzyme (IDE)
and Beta amyloid (Aβ) for a better understanding of the Alzheimier’s disease causes.
▪ Applied techniques: Solid phase peptide synthesis (microwawe peptide synthetizer), Mass
spectrometry (ESI, MALDI), NMR, IR and UV-vis spectroscopy, GC and HPLC.
▪ See annexes for papers.
COMPETENCES
Informatics skills
▪ Good experience with Microsoft Office tools and plugins, Internet browsers and Terminal CLI.
▪ Extensive use of Origin, ImageJ, and Adobe Illustrator on data processing for papers/posters.
▪ Everyday application of bioinformatics resources (Expasy Prosite, BLAST, PDB, 4Peaks).
Social and management skills
▪ Good team spirit and aptitude to work in multicultural environments.
▪ Experience with management of material and instruments orders.
▪ Tutoring of high school, bachelor and master students. University lectures and seminars.
Interests
▪ Sports: Practice of Volleyball, Soccer, Swimming, Rugby, Basketball, Tennis, Skiing…
▪ Cinematography, fine arts, modern and contemporary literature.
EDUCATION
PhD in Molecular Biophysics (thesis defence expected on 03/2014)
10/2009 to present
NEST Laboratory, Scuola Normale Superiore di Pisa, Italy.
Second level Master Degree in Biomolecular Chemistry. 110/110 with honors.
10/2007 to 07/2009
Department of Chemistry, Faculty of Science, University of Catania, Italy.
Bachelor of Science in Chemistry. 110/110 with honors.
10/2003 to 05/2007
Department of Chemistry, Faculty of Science, University of Catania, Italy.
Erasmus stage at École Nationale Supérieure de Chimie de Lille, France.
ANNEXES
SCIENTIFIC PUBLICATIONS
▪
A. Fasoli, F. Salomone, M. Benedusi, C. Boccardi, G. Rispoli, F. Beltram and F. Cardarelli. Mechanistic
insight into CM18-Tat11 peptide membrane-perturbing action by enhanced whole-cell patch-clamp
recording. First name co-authorship. Submitted
▪ D. Pozzi, C. Marchini, F. Cardarelli, F. Salomone, S. Coppola, M. Montani, M. E. Zabaleta, M. A. Digman, E. Gratton, V. Colapicchioni
and G. Caracciolo. Mechanicistic evaluation of the transfection barriers involved in lipid-mediated gene delivery: interplay between
nanostructure and composition. BBA - Biomembranes, 2013. Accepted.
▪ G. Caracciolo, F. Cardarelli, D. Pozzi, F. Salomone, G. Maccari, G. Bardi, A. Capriotti, C. Cavaliere, M. Papi and A. Laganà. Exploiting
the protein corona effect for targeted non-viral nucleic acid delivery. ACS Applied Materials & Interfaces, 2013. Accepted.
▪ F. Salomone, F. Cardarelli, G. Signore, C. Boccardi and F. Beltram. In vitro efficient transfection by
CM18-Tat11 hybrid peptide: a new tool for gene-delivery applications. PLoS ONE, 2013. doi:
10.137/journal.pone.0070108.
▪ G. Caracciolo, D. Pozzi, A.L. Capriotti, C. Cardarelli, F. Salomone and A. Laganà. Exploiting the “protein corona effect” for targeted drug and gene delivery.
Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo., 2013.
▪ D. Pozzi, F. Cardarelli, F. Salomone, C. Marchini, H. Amenitisch, and G. Caracciolo. Effect of cholesterol on the transfection efficiency of DOTAP-containing
lipoplexes. Technical Proceedings of the 2013 NSTI Nanotechnology Conference and Expo., 2013.
▪ L. Albertazzi, F. M. Mickler, G. M. Pavan, F. Salomone, G. Bardi, M. Panniello, E. Amir, T. Kang, K. L. Kilops, C. Brauchle, R. J. Amir and
C. J. Hawker. Enhanced bioactivity of internally functionalized cationic dendrimers with PEG cores. Biomacromolecules, 2012.
doi:10.1016/j.jconrel.2012.09.019.
▪ F. Salomone, F. Cardarelli, M. Di Luca, C. Boccardi, R. Nifosì, G. Bardi, L. Di Bari, M. Serresi and F.
Beltram. A novel chimeric cell-penetrating peptide with membrane-disruptive properties for efficient
endosomal escape. Journal of Controlled Release, 2012. doi: 10.1016/j.jconrel.2012.09.019.
▪ D. Pozzi, V. Orlando, G. Augusti Tocco, S. Biagioni, F. Cardarelli, A. Bifone, F. Salomone, G. Caracciolo. Surface functionalized multicomponent envelope type
nanosysems for improved gene delivery. Nanotech proceeding, 2012. ISBN: 978-1-4665-6276-9.
▪ G. Caracciolo, D. Pozzi, A. L. Capriotti, C. Cavaliere, F. Cardarelli, A. Bifone, G. Bardi, F. Salomone, A. Laganà. Cancer cell targeting of lipid gene vectors by
protein corona. Nanotech proceeding, 2012. ISBN: 978-1-4665-6276-9.
CONFERENCES and STAGES
▪ G. Grasso, F. Salomone, G. R. Tundo, G. Pappalardo, C. Ciaccio, G. Spoto, A. Pietropaolo, M. Coletta, E. Rizzarelli. Metal ions affect
insulin-degrading enzyme activity. Journal of Inorganic Biochemistry, 2012. doi: 10.1016/j.inorgbio.2012.06.010.
th
▪ Biophysical Society 58 Annual Meeting. San Francisco (US). Poster presentation. 02/2014
st
▪ 1 Novartis BioCamp Italia. Siena (It). Scholarship Grant. Award for best case study talk. 10/2013
rd
▪ 3 Conference on Innovation in Drug Delivery. Advances in local drug delivery. Pisa (it). Poster presentation. 09/2013
▪ GRC Gordon Research Conference “Drug Carriers in Medicine and Biology”. Waterville Valley (US).
Poster presentation. Scholarship Grant. 08/2012.
th
▪ Biophysical Society 56 Annual Meeting. San Diego (US). Poster presentation. 02/2012
th
▪ 5 LFD Laboratory for Fluorescence Dynamics Workshop. Irvine (US). 10/2010.
▪ ESONN European School on Nanosciences & Nanotechnologies. Grenoble (Fr). Poster presentation. 09/2010.
▪ EBSA Biophysics Course on Membrane Biophysics & Lipid/Protein Interaction. Bordeaux (Fr). Short talk. 06/2010.
▪ Stage at École Nationale Supérieure de Chimie de Lille, Unitè Materiaux et Trasformations, UMR 8207,
Lille (Fr). 04-08/2006. (Mentor: S. Bourbigot)