Download effects of cvt..e002, a proprietary extract from the north american

89
J Chin Med 13(2): 89-96 , 2002
EFFECTS OF CVT..E002, A PROPRIETARY
EXTRACT FROM THE NORTH AMERICAN
GINSENG (Panax quínquefoJium) ON HEP ATIC
DRUG..l\宜ETABOLIZING ENZYMES IN
C57BL/6J MICE
Yune-Fang Ueng and Chieh-Fu Chen
National Research 1nstitute ofChinese Medicine
Taipei, Ttα Iwan
(Received 14th January 2002 , revised MS received 7' h Februαry 2002, accepted 7' h Februαry2 00刀
CVT-E002 , a proprietary extract from
No的American
ginseng (Panax
quinquφlium) show吋
irnmunomodulating activity. Cytochrome P450 (CYP), UDP-glucuronosyl transferase (UGD , and
glutathione S-transferase (GST) are important drug-metabolizing en可mes . Modulation of drugmetabol 位 ing en勾Imes is a main cause of drug interactions. To assess the possible metabolism-based drug
interaction of CVT-E002 ,
e仔ects
of CVT-E002 on hepatic CYP , UGT, and GST were studied in
C57B Ll6J mice. Treatrnent of mice with 5g1kglday CVT-E002 for three days had no
e能cts
on \i ver
c戶ochrome
bs contents and NADPH-CYP reductase activity. CVT-E002 had no
effect on microsomal CYP catalytic activities ofthe oxidations of7 -ethoxyresorufin , 7-methoxyresorufin ,
microsomal CYP and
be阻o(a)pyrene ，
7-ethoxycoumarin , benzphetamine ,
N-ni甘osodimethylamine，
erythromycin , and
nifedipine in mouse liver. Hepatic microsomal UGT and cytosolic GST activities were not affected by
CVT-E002-甘eatrnent.
Th ese results suggested that CVT-E002 had no effects on hepatic CYP, UGT, and
GST activities in mice under
this 甘eatrnent reglmen. 甘lere
might be low potential of incidence of
metabolism-based drug interaction by CVT-E002
Key words: CVT-E002 , Cytochrome P450 , UDP-Glucuronosyl transferase , Glutathione Stransferase
INTRODUCTION
Modulation of drug-metabolizing enzymes can change the plasma concentrations of drugs and result in
serious drug interactions in humans. Phase 1 and phase 11 drug-metabolizing enzymes play important roles in
the determination ofpharmacological and toxicological effects of drugs
1.
Cytochrome P450 (CYP)-dependent
Cor respondence to: D r. Yune-Fang Ueng , National Research lnstitute ofChinese Medicine , 155-1 , Li-Nong
S甘E仗，
2 , Taipei 112 , Taiwan , R. O. C., Tel: 02必201999 Ext. 6351 , F似 : 02-28264266 , E-mail: ueng@cm a2 3.nricm .edu.tw
Sec.