Download Enzyme-Linked-ImmunoSorbent-Assay

Elisa
Enzyme-Linked-ImmunoSorbent-Assay
Enzyme Linked Immuno-Sorbent Assay (ELISA) is a
powerful technique for detection and quantitation of
biological substances such as proteins, peptides, drug,
antibodies and hormones. By combining the specificity
of antibodies with the sensitivity of simple enzyme assay,
ELISA can provide a quick and useful measurement of the
concentration of one of the above molecules in a biological
fluid (saliva, serum, plasma, vaginal secretions, urine…).
HRP-Linked Antibody
HRP
Detection
Antibody
Target Protein
Currently, there are three major types of ELISA assays
commonly used by researchers :
• direct or indirect sandwich
• competition
• inhibition
TMB
Substrate
Capture
Antibody
Sandwich Elisa
ImmuneHealth can advise on the most appropriate technique to use for a particular application.
ELISA based assays can be developed from scratch, transferred from the client or commercial kits
used.The soft used for data calculation is SoftMaxPro® which provides a four parameters analysis
of the optical densities analyzed by VersaMax reader®.
ImmuneHealth recognises the importance of performing studies not only to the highest standards
of scientific integrity but also in compliance with regulatory requirements. To achieve this we have a
quality system that complies with current good pharmaceutical practices (GCLP) and an independent
Quality Assurance department (reporting directly to the CEO) with many years combined experience
working in a regulated environment.
Research & Services
Elisa
We can create and implement a complete validation of an ELISA
for your company including :
• redaction of SOP
• redaction of the validation plan
• realisation of the validation plan including specificity,
accuracy, intermediate precision (R&R), LOD, LOQ, linearity,
robustness….
• redaction of the validation report.
Rue Adrienne Bolland 8 | B-6041 Charleroi | Belgium
Tel. : +32 (0)71 37 85 00 | Fax : +32 (0)71 37 85 01
[email protected] | www.immunehealth.eu
Research & Services