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Proper Collection of Drinking Water Samples for Coliform Analysis You must use the sample container provided by the laboratory. This container has been sterilized and contains a special chemical (in the form of a white powder) that is required for the test. Find a proper location to take a sample, preferably an outside faucet that does not leak. Remove any hoses (if present) prior to collecting a sample. If an inside faucet is used, remove any aerators or filtration systems (if present) prior to collecting a water sample. Do not collect on windy or rainy days, as this could lead to contamination of the sample. Sterilize the faucet from which the sample is to be collected in order to eliminate any bacteria present on the faucet which may contaminate your sample. The faucet may be sterilized by using one of the following methods: o Use a swab with bleach to wipe the inside and outside of the faucet opening. Rinse the outside lip well with water. o Use a flame (such as a lighter) to sterilize the faucet. Do not use a match, as this could leave soot on the faucet which could contaminate the sample. This method should only be used on outside faucets, as inside faucets could be discolored or tarnished. Turn on the cold water, and allow the faucet to flow for 2 – 3 minutes in order to clear the line. Reduce the flow to a slow, steady, and sprayless stream in order to collect the sample. Remove the sterility seal from the sample container and discard. Remove the cap from the sample container, taking care not to touch the inside of the lid or the lip of the container. DO NOT RINSE THE SAMPLE CONTAINER. The container contains a powder residue which is required for the test. Fill the sample container only to the neck of the container or the fill line, and seal tightly. HEADSPACE Sufficient headspace is needed to properly mix the sample. Make sure the volume is slightly above the fill line, but not to the top of the container. FILL LINE (100 milliliters) 100ml This is the absolute MINIMUM amount of sample we need for analysis. Samples with insufficient volume will be rejected by the laboratory Complete the WATER BACTERIA TEST REQUEST FORM. Make sure that Collection Date and Collection Time are completed, as these are required fields. Submit the sample to the Angelina & Neches River Authority Environmental Laboratory, located at 210 Lufkin Ave, Lufkin, TX, within 24 hours of collection. Samples are accepted Monday – Thursday, 8:00 AM – 4:00 PM. Angelina & Neches River Authority 210 East Lufkin Avenue Lufkin, TX 75901 Phone: (936) 632-7550 Fax: (936) 633-7799 www.anra.org