Download Alemka Markotic

Preparedness for the detection of
emerging-and re-emerging pathogens
in Croatia
Alemka Markotić, MD, PhD
Associate professor
University Hospital for Infectious Diseases
Department for Research
Department for Cinical Immunology
Zagreb
Croatia
History
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The University Hospital for Infectious Diseases was founded in the spring
of 1893, under the name of the City Hospital for Contagious Diseases
Central Diagnostic Laboratory
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Hantaviruses
Serology – acute case detection and
sero-survey
• Puumala and Dobrava viruses
• ELISA (IgM, IgG – reagents provided by
CDC)
• POC
Markotić et al. Nature Med 1995
Markotić et al. Nature Med 1996
Markotić et al. J Med Virol 2002
Markotić et al Scand Infect Dis 2002
Miletić Medved M et al. Lancet 2002
Markotić et al. Croat Med J 2002
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POZITIVAN
GRANIČAN
NEGATIVAN
Molecular detection – human and
rodents samples
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Markotić et al. J Med Virol 2002
Cvetko et al. J Med Virol 2005
Cvetko et al. Am J Trop Med Hyg 2006
Plyusnina A et al. J Med Virol 2011
Tadin A et al. VBZD (under review)
Puumala, Dobrava, Tula, Saarema viruses
Culture based
techniques
Vero
Vero E6
MRC-5
293-HEK
THP-1
Prim. monocytes
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Markotić A et al. J Gen Virol 2003
Markotić A et al. Coll Antropol 2007
Merdić E. et al., Infektološki
glasnik (Croatian Journal of
Infection) 2008; 1: 17-21.
Out of 50 mosquito species
recorded in Croatia, 15 have
potential medical significance.
Anopheles atroparvus, An.
plumbeus, An. sacharovi, Culex
pipiens, and Aedes albopictus are
among the most significant species
that are potential vectors in Croatia.
Climatic changes play an important
role in changing the condition of
habitats, making it possible for new
species to enter certain areas. The
tiger mosquito was recorded in
Croatia in 2004, and it can now be
found in Istria, and more
increasingly in Zadar, Šibenik, Split
and Dubrovnik, with new sites found
on the islands of Cres and Lošinj,
which are very attractive tourist
destinations.
Dengue virus 1-4
(human samples, mosquitos)
AF311958/Brazil 1997
AF513110/Brazil 2001
AF311957/Brazil 1997
AF311956/Brazil 1997
AF514883/Paragvaj 2000
AF514885/Argentina 2000
AF226685/Brazil 1990
• Combined Ag/Ab
POC test
AF226687/Francuska Gujana 1989
AF514878/Paragvaj 2000
Pacijent 1
Pacijent 2
Pacijent 3
AY732474/Tajland 1980
• OneStep RT-PCR
EU179860/Bahrain 2006
1993 4
1 2 AY762084/Singapur
3 41 2 3
tip DENV
FJ225446/Sri Lanka 1997
FJ225448/Sri Lanka 2004
• Nested PCR
AF298807/Obala Bjelokosti 1999
AF180817/Tajland 1964
AY858983/Indonezija 2004
U88535/Otok Nauru 1974
• Sequencing
AB189121/Indonezija 1998
AB189120/Indonezija 1998
AB074761/Indonezija 1988
• Virus isolation
AB204803/Japan 2004
DQ285561/Sejseli 2004
AB195673/Sejseli 2003
DQ285560/Otok Reunion 2004
AB074760/Japan 1943
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AF298808/Djibuti 1998
AF309641/Kambodza 1998
AF350498/Kina 1980
U87411/Tajland 1984
Tick-borne encephalitis
(human samples, ticks)
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ELISA
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PCR
Sequencing
Virus isolation (in the pipeline)
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West Nile Virus
(human samples, mosquitos)
• PCR
• ELISA (temporary)
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Chikungunya virus
(human samples, mosquitos)
• PCR
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Influenza A
(human samples)
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H1, H3, H1N1 2009
POC
PCR
Sequencing
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In the pipeline – molecular dg
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CCHF
Other hemorrhagic fevers
Poxvirus
Rickettsia
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• Virus isolation and immunopathogenesis
research – ntl and intl research resources?
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Greetings from Croatia and
thank you for the attention !
[email protected]