Download Quantification and spatial relationship

Quantification and
Spatial Relationship
Karsten Rodenacker,
Martina Hausner,
Anna A. Gorbushina,
g
Forschungszentrum
Neuherberg
München
Oldenburg
Content
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Introduction
from perception to image analysis
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Measurement
objects, groups of objects
inter- and intra-relationships
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Examples
Conclusion
Introduction
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Perception – recognition –
differentiation – description
Quantification
Relation of qualitative and quantitative
terms
Introduction
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The difficulty NOT to
see something
Introduction
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The difficulty
to see anything
Introduction
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The ease to see the impossible
Introduction
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Quantitative terms
Introduction
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How to relate
qualitative and
quantitative
terms?
Introduction
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Digitisation
Segmentation
Introduction
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Sub sectioning and change of scale
Introduction
Measurement (of one object)
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Extension
Size
Shape
Structure
Introduction
Measurement (of several objects)
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Arrangement
Relation
Neighbourhood
Measurement
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Examples of measurements,
objects and
groups of objects
Spatial relationships
Measurement
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Area
Perimeter
Extension
Measurement
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Shape
Growth shape
Density,
intensity
Measurement
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Extensions
Length (skeleton)
=1621 px
mean
thickness
=2.27 px
Measurement
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Neighbourhood
closing on
filaments
Measurement
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Spatial
Relationship
Delaunay triangulation
nearest neighbours
minimum spanning tree
convex hull
Skeleton neighbourhood
Measurement
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Spatial
Relationship
Example from
pathology
Measurement
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Spatial relationship (objects of different type)
Double marked sludge flocks
Distances to the red phase
Measurement
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Measurement
continuum
Measurement
hierarchy
object
content
location
external
properties
object
pixel
object
content
location
properties
object
object
Example
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Bacterial growth in flow chambers
Differentiation of wild and mutant
bacteria pseudomonas aeruginosa by
CLSM imaging
Example
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Wild (PA) and
mutant (MW)
bacteria
Growth over
time
(slice # = depth)
Example
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Substrate coverage (closing)
Wild type bacteria
Mutant bacteria
Example
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Bacterial growth in flow chambers
Conjugative
genetic
transfer in
bacterial
biofilm
Example
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Quantification of colonies of micro
colonial fungi from sub aerial biofilms
coniosporium sp. and sarcinomyces sp.
under soil (b), sand (s) coverage and in
air (l)
Example
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Colonies of micro colonial fungi
Example
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Colonies of micro colonial fungi
Conclusion
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Perception, description
and measurement of
objects and object groups in images
Exclusions (e.g. texture, filtering,
fractals, etc.)
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Faith and (apparent) truth