Download Membrane Transport III

Membrane Transport III
Chapter 11
Selectivity of a K+ channel
DVD Clip 45
DVD clip 48
Patch Clamp Recording
Patch Clamp Recording
Patch Clamp Measurements of single voltage
gated Na+ channel
3 experiments on
the same patch
cumulative of 144
experiments
Resting Chemical Synapse
Active Chemical Synapse
Neuromuscular Junction in a Frog
Three Conformations of Acetyl Choline receptor
at the neuromuscular junction
DVD Clip 49
Ion Channels at Neuromuscular Junction
Motor Neuron Cell body in the Spinal Chord
Motor Neuron Cell body in the Spinal Chord
thousands of axon
terminals are
stained red
by antibody
that recognizes
a protein in
synaptic vesicles
Intracellular Compartments and
Protein Sorting
Chapter 12
Major Intracellular compartments of
an animal cell
Volumes of major intracellular compartments in
a liver cell
Relative amount of membrane types in 2 cell types
Complex Cortical Network of Endoplasmic
Reticulum
Cross section of liver cell
Topological relationships between compartments
of a eukaryotic cell
Roadmap of
protein traffic
inside cell
Two ways in which a sorting signal can be
built into a protein
Some typical signal sequences
Nuclear Pore complexes perforate the nuclear
envelope
Nuclear pore complexes
Nuclear side of the nuclear envelope
Face on view of nuclear complexes without the
membrane
Side view of 2 nuclear pore complexes
Transport through nuclear pore complexes
occurs through free diffusion and active transport
Nuclear import signal direst proteins to the nucleus
Single amino acid mutation in signal will prevent
import into the nucleus
Nuclear import receptors bind to nuclear porins
and nuclear localization signal of cargo protein
Different nuclear localization signals bind different import receptors
Ran GTP provides energy for nuclear protein import
Ran-GTP controls cargo loading and unloading