Download Supplementary Table 2

Supplementary Table 2- Comparison of protein expression in A375 and M24met by 2D-gel electrophoresis. Corresponding proteins which
are assigned in the 2-D gel in Figure 2. Accession numbers are from the Uniprot database. Vol indicates the volume identified by 2D-gel
electrophoresis n.d =not determined
Protein name
Accession
number
Function
MAGE 4
P43358
Differentiation
Volume (integrated spot
intensities) 106
A375
M24met
1.13
n.d.
PCNA
P12004
Proliferation, DNA repair
1.04
n.d.
Proliferation-associated protein 2G4
Q9UQ80
Proliferation
3.91
1.63
70 kDa subunit of Ku antigen
P12956
DNA repair
0.73
n.d.
Serine/threonine-protein kinase
PAK 2
Q13177
p53 pathway, cell cycle,
metastasis, migration
1.11
2.55
Superoxide dismutase (Mn)
P04179
Redox-regulation
5.41
2.78
Hypoxia up-regulated protein 1
Q9Y4L1
Redox-regulation
6.26
2.7
Comments
May play a role in embryonic development and
tumor transformation or aspects of tumor
progression.
Plays a key role in DNA damage response, being
conveniently positioned at the replication fork to
coordinate DNA replication with DNA repair and
DNA damage tolerance pathways.
Appears to be involved in growth regulation. May
be involved in regulation of intermediate and late
steps of rRNA processing.
Single-stranded DNA-dependent ATP-dependent
helicase. Has a role in chromosome translocation.
The DNA helicase II complex binds preferentially
to fork-like ends of double-stranded DNA in a cell
cycle-dependent manner.
Serine/threonine protein kinase with a role in a
variety of different signaling pathways including
cytoskeleton regulation, cell motility, cell cycle
progression, apoptosis or proliferation.
Destroys toxic superoxide anion radicals normally
produced within the cells
Has a pivotal role in cytoprotective cellular
mechanisms triggered by oxygen deprivation. May
play a role as a molecular chaperone and participate
in protein folding.
Glutathione S-transferase omega-1
P78417
Detoxification
n.d.
4.73
Lysosome-associated membrane
glycoprotein 2
P13473
Lysosomal protein,
metastasis, cell adhesion
0.42
1.16
Cathepsin D
P07339
Lysosomal protein
n.d.
1.43
Vinculin
P18206
Migration, metastasis,
cell adhesion, EMT
1.8
9.04
Annexin A11
P50995
Cell migration
1.11
2.39
Fibronectin
P02751
Cell adhesion, cell
motility, EMT, ECM
n.d.
2.04
Vimentin
P08670
EMT
3.99
16.46
Ubiquitin carboxyl-terminal
hydrolase isozyme L1
P09936
MAP kinase pathway
n.d.
12.32
Glutathione-dependent thiol transferase,
dehydroascorbate reductase, S(phenacyl)glutathione reductase and glutathione Stransferase activities.
Implicated in tumor cell metastasis. May function in
protection of the lysosomal membrane from
autodigestion, maintenance of the acidic
environment of the lysosome, adhesion when
expressed on the cell surface (plasma membrane),
and inter- and intracellular signal transduction.
Protects cells from toxic effects of methylating
mutagens.
Acid protease active in intracellular protein
breakdown. Expressed in lysosomes.
Actin filament (F-actin)-binding protein involved in
cell-matrix and cell-cell adhesion. Regulates cellsurface E-cadherin expression and potentiates
mechanosensing by the E-cadherin complex. May
also play important roles in cell morphology and
locomotion.
Required for mid-body formation and completion of
the terminal phase of cytokinesis.
Fibronectins bind cell surfaces and various
compounds including collagen, fibrin, heparin,
DNA, and actin. Involved in cell adhesion, cell
motility, opsonization, wound healing, and
maintenance of cell shape.
Vimentins are class-III intermediate filaments found
in various non-epithelial cells, especially
mesenchymal cells.
Ubiquitin-protein hydrolase involved both in the
processing of ubiquitin precursors and ubiquitinated
proteins. Involved in negative regulation of the
MAP kinase pathway.