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Chapter 27 Data Sheet: Food microbiology
1. Record your data
2. calculate # bacteria per gram = # colonies / dilution factor
 where 10-1 = 0.1, 10-2 = 0.01, and 10-3 = 0.001
-1
10
# colonies
10-2
10-3
-1
10
Bacteria/gram
10-2
10-3
Average
TSA
VRBA
Copy class data:
Food source
# coliforms/gram
Total bacterial count/gram
Questions for review
1. What type of medium is TSA? What kind of bacterial count does this give you?
2. What type of medium is VRBA? What kind of count does this give you?
3. How many coliforms per gram are allowable for different types of food? Give at least 2 specific
examples
4. How can you detect a coliform bacterium on VRBA agar?
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