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Fishy Frequencies Purpose: To use fish crackers to help build your understanding of natural selection and the role of genetics & gene frequencies in evolution. Standards: a. Students know why natural selection acts on the phenotype rather than the genotype of an organism. a. Students know how natural selection determines the differential survival of groups of organisms. b. Students know a great diversity of species increases the chance that at least some organisms survive major changes in the environment. d. Students know reproductive or geographic isolation affects speciation. Background: These little fish are natural prey of the terrible fish-eating sharks (that’s you). Fish come in two phenotypes gold and ____________. The Gold fish are have a gene that makes then swim faster. New fish are born every “year,” the birth rate equals the death rate. You will simulate births by reaching into the “ocean” of spare fish and choosing the replacement fish randomly. Materials: Two types of fish crackers and container to represent ocean. Data table plus a paper towel to represent the pond. Procedure: Part I – No Selection 1. Randomly choose a study population of 10 fish from the “ocean.” 2. Count the gold and brown fish in your initial population of ten and record your individual data. Do not go on to the next step until the class data has been pooled and recorded. 3. Eat three fish; be sure to choose randomly. 4. Add three fish from the “ocean;” be sure to choose randomly! 5. Count gold fish and brown fish in the restored population and record your data and class data. 6. Again, eat three fish choosing randomly. 7. Add three randomly selected fish, one for each death. 8. Count and record data. Record class data. 9. Repeat steps 6,7, & 8 three more times. 10. Fill in the data Part II – Selection 1. Randomly choose a population of ten fish from the “ocean.” 2. Count the gold and brown fish in your initial population of ten and record your individual data. Do not go on to the next step until the class data has been pooled and recorded. 3. To simulate selection, the predator will no longer eat randomly. You, the terrible fish-eating sharks, much prefer to eat gold fish; you eat ONLY gold fish unless none are available, forcing you to eat brown fish in order to stay alive. Eat three gold fish; if you do not have three, eat brown fish for a total of three! 4. Add three fish form the “ocean”; be sure to choose replacements randomly! 5. Count gold and brown fish in the restored population and record your data and class data. 6. Again, eat three gold fish; if you do not have three, eat brown fish for a total of three. 7. Add three randomly selected fish, one for each death. 8. Count and record data. Record class data. 9. Repeat steps 6, 7 & 8 three more times. 10. Fill in the data table with calculated frequencies (take the number of that color and divide by the total) Data: No Selection Generation # ______ fish # gold fish Total Fish Frequency of ________ Frequency of Gold # gold fish Total Fish Frequency of ________ Frequency of Gold Initial 1 2 3 4 5 6 Selection Generation # ______ fish Initial 1 2 3 4 5 6 Analysis: 1. Make a graph that shows the frequency of color of fish over each generation. 2. What is evolution? 3. What is natural selection? 4. Compare the frequencies of fish when there is no selection. 5. Compare the frequencies of fish when there IS selection. 6. Which fish had a greater chance of survival and why? In other words what gene is favored? 7. Explain what is meant by “differential survival and reproduction” and how this lab demonstrates this them.. 8. Explain what would happen if selective pressure changed and the recessive trait offered a selective advantage (was selected for)? 9. Evaluate this simulation by listing the ways in which it faithfully reflects changes in gene frequencies that might occur in nature AND identifying it shortcomings. Name : ___________________________ Date: ___________ Per. _____ Background: These little fish are natural prey of the terrible fish-eating sharks (that’s you). Fish come in two phenotypes gold and __________. The Gold color is a recessive trait and is genotype ff. These fish are yummy and easy to catch. The __________ trait is dominant and can be genotype FF or Ff. These fish are salty, sneaky and hard to catch. New fish are born every “year,” the birth rate equals the death rate. You will simulate births by reaching into the “ocean” of spare fish and choosing the replacement fish randomly. Data: No Selection Generation # ______ fish # gold fish Total Fish Frequency of ________ Frequency of Gold # gold fish Total Fish Frequency of ________ Frequency of Gold Initial 1 2 3 4 5 6 Selection Generation # ______ fish Initial 1 2 3 4 5 6 Analysis: 1. Make a graph that shows the frequency of color of fish over each generation. 2. What is evolution? 3. What is natural selection? 4. Compare the frequencies of fish when there is no selection. 5. Compare the frequencies of fish when there IS selection. 6. Which fish had a greater chance of survival and why? In other words what gene is favored? 7. Explain what is meant by “differential survival and reproduction” and how this lab demonstrates this them. 8. Explain what would happen if selective pressure changed and the recessive trait offered a selective advantage (was selected for)? 9. Evaluate this simulation by listing the ways in which it faithfully reflects changes in gene frequencies that might occur in nature AND identifying it shortcomings.