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BIOHAZARDOUS and rDNA WASTE PROCEDURES Type of Waste Safety Level Liquid BSL-1 (BL1) + BSL-2 (BL2) BSL-1 (BL1) Proper Collection & Labeling in the Lab Use plastic, leakproof labware which can be sealed. All liquid collection containers must be labeled with the biohazard symbol. Glassware may be used if necessary. Use clear autoclave bags (no symbol) contained within collection receptacles with lids; lids remain closed. Collection receptacles must be labeled with the biohazard symbol Decontamination Method Autoclave or inactivate with an appropriate amount of a commercially available disinfectant (e.g., adding bleach to make a 20% solution) BSL-2(BL2) must validate this process. Autoclave using a Class 5 Chemical Integrator. Autoclave must also be verified monthly as per VT autoclave program*. Disposal After Decontamination After the appropriate contact time this may go down the drain, followed by a water flush Place in black bag before placing into regular trash. (lab employees should place in dumpster) NO bags with biohazard symbol in regular trash Solids BSL-2 (BL2) Use orange autoclave bags contained within collection receptacles with lids; lids remain closed. Both bags and receptacles must be labeled with the biohazard symbol Sharps BSL-1 (BL1) + BSL-2 (BL2) BSL = biosafety level for material potentially infectious to humans or animals . Biohazardous Waste – Any waste capable of causing disease in humans, animals, or plants, or harming the environment Use red, hard plastic Sharps containers with spill-proof lids & biohazard label BL = biosafety level for rDNA material (NIH) Chemical Integrator – Device used to monitor the attainment of one or more of the autoclave operating parameters required for effective sterilization. Decontamination – A procedure which reduces the level of microbial contamination thereby eliminating the risk of transmission of infection. Autoclave using a Class 5 Chemical Integrator. Autoclave must also be verified monthly as per VT autoclave program*. Autoclave using a Class 5 Chemical Integrator. Autoclave must also be verified monthly as per VT autoclave program*. Sharps – Needles, glass slides, tubes, broken plastic, blades, swabs, and other material which can puncture an autoclave bag. Place in Regulated Medical Waste box lined with a red biohazard bag, for pick up by EHS. NO ORANGE OR RED BAGS IN THE REGULAR TRASH. Place in Regulated Medical Waste box lined with a red biohazard bag, for pick up by EHS. NO ORANGE OR RED BAGS IN THE REGULAR TRASH. Regulated Medical Waste – Waste that may contain biological agents capable of transmitting an infectious disease in humans. *Autoclave Use and Verification Program http://www.ehss.vt.edu/programs/BioResearchers/BIO_researchers_autoclave_online.php Virginia Tech EHS Form (8-7-2012)