Download Df(1)sc B57

Early L(1)sc expression: Are there L(1)sc equivalence groups?
Stage 9
8 L(1)sc+ cells
Early Stage 10
All posterior cells L(1)sc+
9-10 L(1)sc+ cells + MP3&4
Total of 11 -12 L(1)sc+ cells
Stage 10:
2 glia underneath MP3
lighter L(1)sc expression
7 lsc+ cells around MP4
Total of 11 l(1)sc+ cells
Lethal of Scute is Present in MPs and their Neuronal Progeny from
Stages 9-11
L(1)Sc
pdm2/nub
merge
Present in PMG (s10-12)
Stage 10
Stage 11
Present in MNB
Present in all MPs (MP4, MP3,
MP1, MP5, MP6) and newly
divided neurons (H-cell, H-cell sib;
mVUM5,6 and iVUM5,6)
Stage 11
Levels are asymmetric between
H-cell and H-cell sib and between
mVUM5 and iVUM5
Stage 12
l(1)sc may play a role in both
neural precursor formation and
establishing different daughter
fates
Lethal of Scute is Present in MNB
L(1)sc
wor
Reduced Numbers of Neurons are Present in AS-C Mutants
Stage 14
Stage 16
Df(1)scB57
Wild -type
Stage 12
6 elav+ cells present from late 11-16
Suggests that reduced numbers of neurons not due to cell death, but
less neurons are generated
AS-C Genes are Required for wor Expression
merge
Df(1)scB57
Wild -type
wor
wor: MNB
Suggests MNB does not form and does not produce its 8 progeny
CG8965 and wrapper are unaffected in AS-C mutants
CG8965
scB57:
wrapper
5 CG8965+ cells/seg
3.13 wrapper+ CG8965- cells/seg
n= 8 seg
High levels of wrapper did not always equal CG8965+ cells
wt:
3 PMG/seg @ s14
n=2
AS-C genes are Required for tbh Expression
Tkr
(mVUM6; iVUM6)
merge
Cas
(mVUM4,5; iVUM4,5)
Df(1)scB57
Wild -type
Tbh
(mVUMs)
4
5
6
60%
50%
mVUM
iVUM
Tbh Cas Tkr
Suggest that mVUM6 and iVUM6 are present 60%
AS-C Phenotype : Precursor Formation and Neuron-Specific Control
AMG
AMG
AMG
AMG
PMG
PMG
PMG
PMG
Neither Notch nor AS-C
Wild-type
L(1)sc
AMG
AMG
MP1
MP3
MP4
MP5
MP6
Notch
MNB
Both L(1)sc and Notch
Numb
MP1
MP1
H-cell
H-cell
sib
mVUM
iVUM
MNBp
AS-C Phenotype : Precursor Formation and Neuron-Specific Control
AMG
AMG
AMG
AMG
PMG
PMG
PMG
PMG
AS-C mutant
Neither Notch nor AS-C
L(1)sc
AMG
AMG
MP1
MP3
MP4
MP5
MP6
MNB
Notch
Both L(1)sc and Notch
X
MP1
MP1
H-cell
sib
X
iVUM
Numb
4 pdm2/nub+ Neurons are Present in AS-C mutants
merge
Wild -type
pdm2/nub
MP1
MP1
Df(1)scB57
MP1
Stage 14
pdm2/nub: MP1s, H-cell, H-cell sib
H-cell
MP3
H-cell
sib
MP4
MP5
mVUM
MP6
iVUM
AS-C Genes are Required for ple Expression
CG13565
(H-cell sib)
Lim3
(MP1s)
Df(1)scB57
Wild -type
ple
(H-cell)
MP1
MP1
pdm2/nub: MP1s, H-cell & H-cell sib present
MP1 and H-cell sib gene expression unaffected
AS-C genes required for H-cell specific gene expression
MP3
MP1
H-cell
H-cell
sib
AS-C mutants
Df(1)sc B57
D(f)sc 19
Xac
Xsc
l(1)sc
ase
X
X
Xsc l(1)sc
ase
X-ray derivative of In(1)ac3
ac
pt mutation causes sc to lack C term
Sc protein nonfunctional
Rearrangement that separates all 5’ seq of ac
X
X
Scute is Present in MPs, but not Neurons from Stages 10-11
Sc
pdm2/nub
merge
Stage 10
Present in PMG (s10-11)
Stage 11
Stage 11
Stage 11
Present in MP3, MP5, MP1,
MP6, and MNB prior to division
and is absent from newly
divided neurons
May play a role in neural
precursor formation, but not in
establishing different daughter
fates
Achaete Expression
Ac
pdm2/nub
merge
Stage 10
Present in MP1 and transiently in
MP5, MP6, and MNB
Stage 11
Stage 11
Stage 12
Remains on in MP1 neurons to
end of embryogenesis
May play a role in precursor
formation and in MP1 neuron
development