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Download Protein Primary antibody used (Dilution) Reference for the antibody
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Protein Primary Reference for Method of antibodies Method of checking the Secondary antibody used antibody the antibody generation specificity of antibodies (Dilution) used used (Dilution) PfTPxGl rabbit raised Chaudhari et al. Polyclonal antibodies Western blot of the parasite Goat anti-Rabbit IgG (H+L) anti-PfTPxGl 2012 and this generated against full lysates, (1:250) study length protein Immunoprecipitation of the (Invitrogen™) (1:1000) / Goat parasite lysates Alexa Fluor® 568 anti-Rabbit IgG (H+L) Alexa Fluor® 488 (Invitrogen™) ACP rabbit raised This study Polyclonal antibodies Western blot of the parasite Goat anti-Rabbit IgG (H+L) anti-ACP generated against the lysates (1:250) synthetic peptide Alexa Fluor® 568 (Invitrogen™) (1:1000) corresponding to the amino acids from 41-55 of the ACP protein sequence UROD mouse raised Nagaraj et al. Protein-A purified Western blot of the parasite Goat anti-Mouse IgG (H+L) anti-UROD polyclonal IgG antibodies lysates 2009 (1:250) Alexa Fluor® 568 (Invitrogen™) (1:1000) generated against UROD protein lacking N-terminal 43 amino acids, PfFC mouse raised Varadharajan et Polyclonal antibodies Western blot of the parasite Goat anti-Mouse IgG (H+L) anti-FC lysates, al. 2004 generated against FC Alexa Fluor® 568 protein lacking first 113 Immunoprecipitation of the (Invitrogen™) (1:1000) amino acids parasite lysates mouse raised Siddiqui et al. Polyclonal antibodies Western blot and anti-BiP 2013, Kaderi generated against a immunoprecipitation of the Alexa Fluor® 568 (1:100) Kibria et al. 2015 synthetic peptide (1:250) BiP parasite lysates (Author Goat anti-Mouse IgG (H+L) (Invitrogen™) (1:1000) corresponding to the last 11 communication) carboxy terminus amino acids of PfBip conjugated with KLH (Thermo Fishcher Scientific) GRASP mouse raised Siddiqui et al. Polyclonal antibodies Western blot and anti-GRASP 2013, Kaderi generated against a immunoprecipitation of the Alexa Fluor® 568 (1:100) Kibria et al. 2015 synthetic peptide corresponding to the 14 parasite lysates (Author Goat anti-Mouse IgG (H+L) (Invitrogen™) (1:1000) communication) amino acids from 529-543 of PfGRASP conjugated with KLH (Thermo Fishcher Scientific) EMP1 rabbit raised Ganguly et al. Polyclonal antibodies Western blot of the parasite Goat anti-Rabbit IgG (H+L) anti-EMP1 2015 generated against 290 lysates (Author Alexa Fluor® 568 amino acids VARC communication) (Invitrogen™) (1:1000) (1:500) (cytoplasmic domain) region from the EMP1 protein sequence KAHRP mouse raised Ganguly et al. Polyclonal antibodies Western blot of the parasite Goat anti-Mouse IgG (H+L) anti-KAHRP 2015 generated against 49 amino lysates (Author Alexa Fluor® 650 (1:150) acids K2A1(central lysine communication) (Invitrogen™) (1:400) rich region) region from the KAHRP protein sequence GFP mouse raised Roche Life Mixture of two monoclonal Western blot of the wild Goat anti-Mouse IgG (H+L) anti-GFP antibodies, both clones are type and mutant GFP Alexa Fluor® 568 purified Mouse IgG1ĸ (Invitrogen™) (1:1000) (1:250) Sciences proteins
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