Download 演題申込書 - 第10回日本―中国合同皮膚科学術会議

第 10 回日本-中国合同皮膚科学術会議
演 題 申 込 書
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発表者と共同演者（発表者にはﾁｪｯｸをつけてください。
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Presentation type
□Oral presentation
□Poster
(Final decision will be made by the program committee)
□CPC
Category (please mark topics best describing your presentation)
□Appendages' Diseases
□Bacterial and Viral Infection
□Basic Research
□Benign and Malignant Tumors
□Bullous Diseases
□Collagen Diseases and Vasculitis
□Cosmetic Dermatology
□Dermatitis and Skin Allergy
□Fungal Infection
□Genetic Disorders
□Metabolic and Endocrine Disorders
□Photodermatoses
□Pigmentary Disorders
□Psoriasis
□Sexually Transmitted Diseases
□Therapies
□Miscellaneous
見本１
Bone morphogenetic protein-2 modulates Wnt and frizzled expression and upregulates Wnt-beta-catenin signaling
pathway in normal keratinocytes
Lujun YANG1, Kenshi YAMASAKI1, Yuji SHIRAKATA1, 2, Xiuju DAI1, Sho TOKUMARU1, and Koji
HASHIMOTO1.
1 Department of Dermatology, Ehime University Graduate School of Medicine, Ehime, Japan
2 Center for Regenerative Medicine, Ehime University Graduate School of Medicine, Ehime, Japan
Background: Bone morphogenetic protein-2 (BMP-2) and Wnt are involved in the development of skin and skin
appendages as a morphogen. However, if there is crosstalk between BMP-2 and the Wnt/β-catenin signaling pathway
is not clear.
Objective: We examined BMP-2-dependent expression of Wnt and its receptor frizzled in normal human
keratinocytes.
Methods: The mRNA expression of the Wnt and frizzled families was analyzed by reverse transcription-polymerase
chain reaction (RT-PCR) or ribonuclease protection assay. β-Catenin expression was measured using RT-PCR and
Western blotting. TCF/LEF activity was analyzed using the luciferase reporter assay.
Results: We detected the expression of Wnt-2b/13, -4, -5a, -5b, -7a, -7b, and -10a, frizzled-1, -4, -5, -6, -8, -9, and -10,
MFRP, and SFRP-1/SARP-2 in keratinocytes. BMP-2 increased Wnt-2b/13, -5b, and -7b, and frizzled-6, -8, and -10.
Conversely, BMP-2 suppressed Wnt-10a and SFRP-1/SARP-2. Although Wnt-4 expression was not affected by
BMP-2 in confluent conditioned keratinocytes, BMP-2 suppressed cell density-dependent Wnt-4 induction. The
transcriptional activity of T-cell factor/lymphoid enhancing factor (TCF/LEF), which is a target of the canonical Wnt
pathway, was upregulated by BMP-2 in both time- and dose-dependent manners. However, BMP-2-dependent
differentiation of keratinocytes suppressed TCF/LEF transcriptional activity.
Conclusion: These results suggest that BMP-2 modulates the expression of molecules involved in Wnt signaling, and
activates the canonical Wnt pathway in normal human keratinocytes. Moreover, Wnt signaling may be influenced by
the fate of keratinocytes, such as proliferation, migration, and differentiation.
見本２
Capillary leak syndrome complicating generalized pustular psoriasis: possible role of circulating vascular endothelial
growth factor
Yukiko INOUE, Yuji SHIRAKATA, Koji HASHIMOTO
Department of Dermatology, Ehime University Graduate School of Medical Science, Ehime, Japan
Introduction: Capillary Leak Syndrome was first described in 1960 by Clarkson et al and characterized by chronic
recurrent episodes of a triad of hypotension, hypoalbuminemia and hemoconcentration. It is caused by increased
capillary permeability and consists of the shift of fluid and proteins from the intravascular to the extra vascular
compartment with subsequent hypovolemic shock. We report a case of Capillary Leak Syndrome accompanied with
Generalized Pustular Psoriasis.
Case Report: A 55-year old man, who had been diagnosed as Generalized Pustular Psoriasis for 25 years, admitted
urgently with acute cardio respiratory failure and renal failure .He had developed pustular eruption on his trunk
accompanied by generalized edema. He presented hypotension, anorexia, oliguria, high fever and disturbance of
consciousness. Laboratory investigations revealed leukocytosis, (32000/l), an increase in both hematocrit (54%) and
hemoglobin (17.4 g/dl), and decrease in albumin (2.9 g/dl) .Liver and renal function was also abnormal. His chest
radiograph showed extensive bilateral alveolar shadowing consistent with profound pulmonary edema, which
suggested capillary leak syndrome. He was transferred to the intensive care unit where he was provided ventilator
support and continuous hemodiafiltration and treated with intravenous fluids, dopamine, albumin, predonisolone 60
mg/day and etretinate 50 mg/day. Three weeks later he recovered enough to quit mechanical ventilation and
continuous hemodiafiltration.
Comment: We diagnosed our present case as Capillary Leak Syndrome accompanied with Generalized Pustular
Psoriasis, because it showed hypotension, hypoalbuminemia, hemoconcentration, generalized edema and renal failure.
In 1994, Detmar et al reported that psoriatic skin express strikingly high amounts of vascular endothelial growth factor
(VEGF). In 2002, Creamer indicated that patients with severe psoriasis (PASI score>30, Generalized Pustular
Psoriasis) had significantly higher levels of plasma VEGF compared with patients with moderate psoriasis (PASI
score<30). Therefore, we measured the concentration of plasma VEGF. On day 3 the concentration of VEGF was
1840.0 pg/ml and it was 77.2 pg/ml on day 20. Creamer et al suggest that VEGF entered the systemic circulation and
acts on micro vessels to mediate hyper permeability. We suppose that high level of VEGF in early phase of
Generalized Pustular Psoriasis is associated with a case of Capillary Leak Syndrome. Capillary Leak Syndrome is
known as severe disorder with a high mortality rate. We suggest that there is some possibility of predicting the onset
of Capillary Leak Syndrome by measuring of the plasma VEGF in patients with Generalized Pustular Psoriasis.