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Ion or Isotope Lab IONS are atoms with a CHARGE. An atom will have a charge if the number PROTONS and ELECTRONS are NOT EXACTLY the same. The number of PROTONS ALWAYS identifies the element on the periodic table. If there are more protons than electrons, there will be a POSITIVE charge. If there are more electrons, there will be a NEGATIVE charge. The number of additional protons or electrons will determine the charge. For example, if there are two extra protons - the charge will be 2+. If there are three extra electrons – the charge will be 3-. ISOTOPES have different numbers of NEUTRONS. You can calculate the number of neutrons by subtracting the ATOMIC NUMBER from the ATOMIC MASS NUMBER. If the NEUTRON NUMBER does not match the number of neutrons that you got when you subtracted, then that atom is an ISOTOPE.