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NMEICT-MHRD (Govt. of India) Project on - Creation of e-Contents on Fermentation Technology
Module-6: Primary and Secondary screening
Question banks
Define the following terms

Screening

Primary screening

Secondary screening
Answers the following

Exemplify primary screening of amylase producing microorganisms

Exemplify primary screening of cellulase producing microorganisms

Exemplify primary screening of organic acid producing microorganisms

Exemplify primary screening of antibiotics producing microorganisms

Exemplify primary screening of vitamins producing microorganisms

Exemplify primary screening of amino acids producing microorganisms

Exemplify primary screening of growth factor producing microorganisms

Exemplify primary screening of microorganisms that utilize specific carbon source

Exemplify primary screening of microorganisms that utilize specific nitrogen source

Enlist criteria of secondary screening

What is the strategies for isolation of industrially important microbes

Enlist criteria used for choice of organisms
Statefollowing statements are TRUE or FALSE
1. Secondary screening should be qualitative or quantitative in its approach
2. Secondary screening should not give information about the evaluation of the true
potential of the microorganisms for industrial usage
3. Secondary screening should not determine whether microorganisms are actually
producing new chemical compounds not previously described
4. Secondary screening should reveal whether there is pH, aeration or other critical
requirements associated with particular microorganisms, both for the growth of the
organism and for the formation of chemical products
5. It is not important for any organisms which is to be employed for production purpose
to have genetic stability and its amenability to genetic manipulation
Project control No: RE-02091011297, Christ College, Rajkot, Gujarat, India
NMEICT-MHRD (Govt. of India) Project on - Creation of e-Contents on Fermentation Technology
6. Industrially important stain need not to have high yielding capacity
7. Industrially important strain should have stable biochemical characteristics
8. Industrially important strain should not produce undesirable substances
9. Industrially important strain should be easily cultivated
Project control No: RE-02091011297, Christ College, Rajkot, Gujarat, India