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Biomolecular and Cellular Engineering
Microbes
Metabolic
Engineering
Synthetic
Biology
Enzymes
Protein
Engineering
Biomolecules
Directed
Biopolymer
Evolution
Antibody
Nano-Devices, Sensors, Environmental,
Biosynthetic and Biomedical Applications
Research Interest
Biomolecular Tools
Artificial Cellulosomes
1. Found in anaerobic microbes
2. Hydrolyze cellulose up to 50-fold faster due to synergy
3. Interaction between dockerin to cohesin is species specific
Bayer et al.,
2006
Synthetic Yeast Consortia
11000
Remaining Sugars (mg/L)
YEAST
YEAST
YEAST
YEAST
10000
9000
His6 Tag
His6 Tag
Control
8000
YEAST
YEAST
Free Enzymes
Cellulosome
C
T
CBD
7000
F
AGα1
0
20
40
Time (hrs)
60
80
YEAST
1200
25
Control
Control
Free Enzymes
Free Enzymes
Cell Numbers (10 6 /mL)
1000
Ethanol (mg/L)
His6 Tag
Cellulosome
800
600
400
20
Cellulosome
15
10
200
0
5
0
20
40
Time (hrs)
60
80
0
20
40
Time (hrs)
60
80
Virus detection and drug discovery
Influenza
In situ Monitoring of Virus Replication
Using Molecular Beacons
Translation
Uncoating
RNA replication
Nucleus
Fixation with para-formaldehyde and permeabilization Triton X-100
In vivo detection of Coxsackie B6 virus in BGMK cells
Cells infected with 103 PFU, M.O.I.≈ 0.5 (15 min to 18 hr)
PlasDIC (t = 0 min)
PlasDIC (t = 18 hr)
Yeh et al. PNAS, 17522-17525, 2008
Acknowledgement
9 Graduate Students and 1 Postdoc
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