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Quantification of Niacin and Folate Contents in Peanuts. M.L. EAST*, L.L. DEAN, T.H. SANDERS, Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, NC 27695 and USDA, ARS, Market Quality and Handling Research Unit, Raleigh, NC 27695. Peanuts (Arachis hypogaea L.) are known to be sources of several important B-vitamins, including niacin and folate. Recent research has shown that therapeutic doses of niacin are beneficial for vascular health; therefore, determination of the concentrations found in current varieties in production and potential breeding lines is needed. Folate is a term for a related group of compounds with vitamin activity important in DNA biosynthesis. Adequate levels of folate intake have been found to reduce levels of a variety of syndromes, most notably fetal neural disorders. Current information about the concentrations and the specific types of folates present may position peanuts as an important source of this nutrient. For this study, we analyzed a series of samples from the 2007 and 2008 UPPT and the 2005 Core of the Core peanut germplasm collection to compare levels of niacin and folates across 10 locations. For the niacin assay, 39 total UPPT Florunner and NC7 samples were analyzed using the AOAC non cereal foods method. Selected samples from the Core of the Core were also analyzed. Samples were replicated within and between years. Niacin concentration (mg/100g) ranged from 8.20 to 25.8 with an average of 16.1 for the 2007 UPPT samples, 17.7 for the 2008 UPPT samples, and 16.8 for the Core of the Core samples. The average niacin concentration in Florunner and NC7 samples increased from 14.6 and 17.4 in 2007 to 16.4 and 18.9 in 2008, respectively. Regardless of peanut type, the average concentration of niacin increased from 16.1 in 2007 to 17.7 in 2008. For the folate assay, Core of the Core samples were analyzed using the AOAC microbiological method. Folic acid concentration (mg/100g) ranged from 102.8 to 235.9, with an average folate content of 163.3. Environmental effects may influence the content observed between peanut type and location; however, specific effects are still being investigated. These results suggest that germplasm/varietal differences may be sufficient for increases in levels of these vitamins through conventional breeding.