Download characteristics of cell line:

CHARACTERISTICS OF CELL LINE:-
TYPES OF CELL LINE: NORMAL CELL
 TRANSFORMED CEL
 CONTINUOUS CELL
NORMAL CELL LINE:It is defined as the cell line which divides a limited number of times
before loosing their ability to proliferate.
CELL LINE
CELL TYPE
ORIGIN
SPECIES
MARKER
C7
Endocrine
Hypothalamus
Mouse
Neurophysin
CHARACTERISTICS OF NORMAL CELL
LINE: Susceptible to human viral infection
 These cell have the ability to reproduce correctly, stop reproducing
whenever necessary.
 Remain in specific location
 Become specialized for specific function
 Self destruct when necessary.
TRANSFORMED CELL: It is defined as the conversion of normal healthy cell into a
TUMOROUS or CANCEROUS cell.
 It can be caused by exposure to a mutagen or carcinogen.
 For e.g.:- Ethidium Bromide, Formaldehyde, DEPC ( Di ethyl pyro
carbonate), UV radiation
 Proto oncogenes are normal healthy functional genes, which have
the potential to get converted into oncogenes upon exposure to a
carcinogen.
E.g.CELL LINE
CELL TYPE
ORIGIN
SPECIES
MARKER
GH3
Endocrine
Pituitary
Tumour
Rat
Growth
hormone
CHARACTERISTICS OF TRANSFORMED
CELLS: IMMORTALIZATION:1. The tumour/cancer has indefinite life span either due to mutation or
deletion of senesce genes.
2. It may also occur due to mutation in proto oncogenes or over expression of
oncogenes.
 ABERRANT GROWTH CONTROL:1. The cells will lose the growth control i.e. they will grow beyond saturation
density and will form heaps of cells on a confluent monolayer.
2. The cells may be immortal but still have there growth control intact.
3. E.g.- 3T3 mouse cell lines
 METASTASIS:1. It is the property to invade normal healthy cell.
2. E.g.- Leukaemia, blood cancer
3. Due to this property the tumour is able to proliferate at much higher
rate
 HIGH PLATING EFFICIENCY:They grow at a very high rate hence make the plate confluent in low time.
 CELL SURFACE MODIFICATION:1. L-CAM’s are very less expressed that is cell to matrix/ substrate interaction is
inhibited.
2. N- CAM’s are over expressed i.e. cell to cell interaction increase and the heaps of
cells are formed.
 SHORTER POPULATION DOUBLING TIME:Due to very short doubling time the tumour will replicate at a rapid rate.
 GROWTH FACTOR:1. The tumour is very less dependent upon the serum and the growth
factor of the host, because tumour secretes its own growth factor
• TiGF
• TAGF
CONTINUOUS CELL LINE:When a normal cell line undergoes transformation and acquires the
ability to divide indefinitely then it is known as continuous cell line.
CELL LINE
CELL TYPE
ORIGIN
SPECIES
MARKER
MDCK
Epithelium
kidney
Dog
Domes,
transport
CHARACTERISTICS OF CONTINUOUS
CELL LINE: Fast growth
 Grow more in suspension condition
 Ability to grow up to higher cell density
 Different in phenotype from donor tissue
 Homogeneous cell population
 Smaller, more rounded, less adherent with a higher nucleus/
cytoplasm ratio.