Download Lecture plan- Basic Techniques in Microbiology Lab

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Lecture plan- Basic Techniques in Microbiology Lab
Lecture-1: Laboratory safety- general precautions, chemical hazards, microbiological hazards,
hazards symbols/ Introduction to the Compound Microscope (lecture + lab)
Lecture-2: Microscopic examination of living bacterial preparations by using Hanging –Drop
procedure/stained cell preparations (lecture + lab)
Lecture-3: Microscopic Measurement of Microorganisms/ Simple, Gram/Spore/Capsule staining
(lecture + lab)*
Lecture-4: Preparation, dispensing and sterilization of nutrient broth and agar media (lecture +
lab)*
Lecture-5: Culture transfer technique/ Different plating methods (lecture + lab)*
Lecture-6: Techniques for the isolation (from Food sample/Water sample/Environmental
sample), preservation and maintenance of pure culture (lecture + lab)*
Lecture-7: Use and function of differential and selective media and observation of cultural
characteristics of bacteria on various media (lecture + lab)*
Lecture-8: KIA test / Oxidase test and Urea hydrolysis (lecture + lab)*
Lecture-9: Citrate utilization test, Catalase test and Nitrate reduction/Indole production, Gelatin
hydrolysis, Methyl red test and Voges-Proskauer test (lecture + lab)*
Lecture-10: Processing of sewage sample for the detection of bacteriophages/Isolation of
bacteriophages from sewage sample (lecture + lab)*
Lecture-11: Effect of heat(dry and moist), pH, salinity and UV on bacterial growth/ Isolation of
drug resistant mutant(lecture + lab)*
Lecture-12: Serum separation from fresh blood sample/HBsAg assay in patient serum sample by
ELISA(lecture + lab)*
*NEED 2 DAYS TO COMPLETE (DAY-1: INOCULATION; DAY-2: OBSERVATION)
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