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Gene silencing in plants. LB p 35S GFP intron PFG RB LB GFP p 35S RB Transcription Transcription Poly A GFP intron CAP Poly A PFG CAP DICER GFP gene silencing mRNA degradation Translation GFP CAP RISC PFG Poly A CAP Poly A GFP Poly A CAP No Fluorescence Fluorescence 1.a. Gene silencing of transiently expressed sequences. UV Nb LB p 35S GFP LB RB LB p 35S p 35S GFP GFP intron RB PFG RB 1.b. Triggering gene silencing of established transgene by transient expression. UV GFP LB p 35S GFP intron PFG RB 1.c. Virus-induced gene silencing (VIGS) Nt Nt Nt Agroinfiltration PTGS PDS Sistemic PTGS pTRV1 LB p 35S MP RpRd 16K pTRV2 LB p 35S CP PDS RZ RB RZ RB 2. Systemic gene silencing. UV LB p 35S GFP GFP Agroinfiltration PTGS GFP GFP intron PFG RB GFP Sistemic PTGS 3.a. Suppressing gene silencing of transiently expressed sequences. LB LB p 35S p 35S LB GFP p 35S GFP intron HcPro RB PFG RB RB UV Nb LB p 35S GFP LB RB LB p 35S p 35S GFP GFP intron RB PFG RB 3.b. Silencing of trangenes or endogenous genes cannot be established in the presence of silencing suppressor. UV GFP LB p 35S GFP intron PFG RB LB p 35S LB GFP p 35S intron HcPro PFG RB RB 3.c. Suppression of gene silencing mediated by virus infection. PVY infection. Plant silenced for the Magnesium chelatase gene. Plant silenced for the Magnesium chelatase gene.
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