Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
PROBIOTIC LAB CAUSED PERSISTENT CHANGES IN THE CANINE JEJUNAL MICROBIOTA Rinkinen, M. L.1, Manninen. T.2, Beasley, S.2 and Saris, P. E.2 1 Department of Clinical Veterinary Sciences, University of Helsinki, Helsinki, Finland 2 Department of Applied Chemistry and Microbiology, University of Helsinki, Helsinki, Finland The aim of the study was to investigate the stability of potential probiotic lactic acid bacteria (LAB) in the canine small intestine. Most of the earlier studies have examined the competitive exclusion of pathogenic bacteria, whereas reports of the potential effects on the endogenous LAB population are scarce. Five lactic acid bacteria strains isolated from dog faeces (Lactobacillus fermentum, L. salivaruius, L. rhamnosus, L. mucosae and Weissella confusa) were evaluated in this study. The strains were fed to five permanently fistulated dogs for 7 days and the stability of the LAB strains were monitored for 17 days by plating jejunal content on mLBS. The chromosomal DNA of the bacterial colonies was cloned by PCR and run in DGGE to detect the living bacteria passing through the intestine. The effect of the supplemented LAB on the normal intestinal LAB microbiota was determined by DGGE. All the fed LAB strains survived the passage through the upper GI tract but disappeared within 7 days after the supplementation had ceased. However, a significant and persistent change in the indigenous LAB microbiota caused by the fed LAB lasted more than 17 days after the cessation of LAB supplementation. We conclude that this long-term modification of the indigenous intestinal LAB microbiota may be an important mechanism for probiotic LAB to exert their beneficial health effects.