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Ch. 10.1 Mendel’s Discoveries Objectives: 1. 2. Compare and contrast the blending hypothesis and the particulate hypothesis of inheritance. Describe the methods Mendel used in his plant-breeding experiments. Vocab: Trait Genetics Cross-fertilization Gregor Mendel • Austrian monk • “Father of Modern Genetics” (Study of heredity) • Breed pea varieties. Pre-Mendel Blending Hypothesis: Offspring have a blend of parent traits. Ex: Red x White flower --> Pink flowers --> Pink in next generation too. Problem: Red and white flowers appear again in future generations; traits are not really blended. Background Vocab. True-breeds: pure gene lines – offspring match parent Self-pollination: pollen from flower fertilizes the same plant Cross-fertilization: pollen will fertilize a different plant Hybrid: Cross between organisms with different traits (blonde hair & brown hair) Trait: Physical characteristics Mendel’s Pea Traits Cross-Pollinating Mendel’s Crosses TRAIT 1: Seed Shape P Cross: Round v. Wrinkled F1 Phenotype: Round *P = parents *F1 = “filial” = “son”= off-spring Mendel’s Crosses TRAIT 2: Seed Color P Cross: Green v. Yellow F1 Phenotype: Yellow Mendel’s Crosses TRAIT 3: Flower Color P Cross: Purple v. White F1 Phenotype: Purple Mendel’s Crosses TRAIT 4: Pod Shape P Cross: Inflated v. Pinched F1 Phenotype: Inflated Mendel’s Crosses TRAIT 5: Pod Color P Cross: Green v. Yellow F1 Phenotype: Green Mendel’s Crosses TRAIT 6: Flower Position P Cross: Axial v. Terminal F1 Phenotype: Axial Mendel’s Crosses TRAIT 7: Plant Height P Cross: Tall v. Short F1 Phenotype: Tall Mendel’s Conclusions Biological inheritance is determined by chemical factors passed from one generation to the next (Particulate hypothesis) – Geneticists now refer to these factors as genes – Genes can come in more than one form, each form is an allele ex. B or b (The “B” gene w/ 2 alleles) Ch. 10.2 Mendel’s Genetics Objectives: 1. 2. 3. 4. Explain Mendel’s principle of segregation. Describe how probability applies to genetics. Contrast genotype and phenotype. Explain Mendel’s principle of independent assortment. Vocab: Hybrid Monohybrid cross Allele Homozygous Heterozygous dominant Recessive Punnett square Phenotype Genotype Testcross Dihybrid cross http://www2.edc.org/weblabs/WebLabDirectory1.html More Genetics Vocab • Homozygous: two identical alleles (AA or aa) • Heterozygous: two different alleles (Aa) • Phenotype: Physical appearance • Genotype: Genetic make-up – Homozygous dominant (AA) – Homozygous recessive (aa) – Heterozygous (Aa) Mendel’s Principles 1. There are alternative forms of genes (alleles) 2. There are 2 alleles for each trait (BB,Bb, bb) * we now know there can be more. 3. Some alleles are dominant; some recessive. Dominance: a recessive allele will be masked by a dominant allele 4. Principle of SEGREGATION: Alleles for each trait segregate (separate) during gamete formation (Anaphase I of meiosis) Monohybrid Crosses = Parents differ in only 1 trait Results: F1: all purple F1 purples self- fertilize to see if white trait was lost. F2: 75% purple; 25% white White trait NOT lost! Diagram that shows all possible outcomes of a genetic cross Phenotype & Genotype Ratios Phenotype Ratio 3 Purple: 1 White Genotype Ratio 1 PP: 2 Pp: 1pp -Reveals unknown genotype thru. Ratios - Unknown (Dominant phenotype) x homozygous recessive Mendel’s DIHYBRID CROSSES Independent Assortment: Alleles for different traits do not influence each other’s segregation. Dihybrid Cross Traits are inherited separately. F2: 9:3:3:1 Ratio