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10.6 Representing variation
graphically
Teacher notes
OCR Biology A
Comparing two populations – a statistical
approach
Specification reference

4.2.2 (f)
Learning outcomes
After completing the worksheet students should be able to:

understand the reasons for selecting statistical routines to analyse data

carry out one statistical analysis (t-test) with confidence.
Introduction
Variation between individuals provides many opportunities for experimental studies. These
experimental approaches often generate large amounts of data which need to be analysed.
There are a range of methods, graphical and statistical, which can be used in such an analysis.
Selecting the correct approach is often an area which causes difficulties for students. Once the
appropriate methods have been selected students find it difficult to apply the routines correctly.
The aim of this sheet is to explain where each test might be most appropriate, and to give a
detailed explanation of one such test.
Teacher notes

This sheet assumes a knowledge of variation but does not really cover the topic. It is focused
more on how to carry out a statistical routine.

First list the types of routines commonly encountered, and use the summary table to identify
clear features which distinguish when each of the tests are used.

The support sheet provides a partially worked example of the students t-test. Work through
this with the students.

You may choose not to construct a graph depending on time, but it is a useful visual start
before approaching the statistics. Discuss the fact that the graph indicates two distinct
groups, but cannot provide convincing evidence.

The students t-test is approached in a step by step manner. The answers are supplied below
to help you support the student. Work through the example either individually or as a class.

Finally the exam-style questions are short and approach aspects of the test.
Answers
Task
Graphing the data.
Shell Height (cm)
Sheltered beach population
Exposed beach population
1.51 – 1.75
0
1
1.76 – 2.00
1
3
2.01 – 2.25
1
4
© Oxford University Press 2015
www.oxfordsecondary.co.uk/acknowledgements
This resource sheet may have been changed from the original
1
10.6 Representing variation
graphically
Teacher notes
OCR Biology A
Shell Height (cm)
Sheltered beach population
Exposed beach population
2.26 – 2.50
3
3
2.51 – 2.75
4
2
2.76 – 3.00
3
2
3.01 – 3.25
3
0
3.26 – 3.50
1
0
Graph should show two distinct populations.
Table of values.
Sheltered beach
Number of animals
tested (n1)
Exposed beach
17
Number of animals
tested (n2)
15
Mean ( x 1)
2.76
Mean ( x 2)
2.26
Standard deviation(σ1)
0.374
Standard deviation(σ2)
0.870
Standard deviation
squared (σ12)
0.140
Standard deviation
squared (σ22)
0.137
t  3.76
degrees of freedom  30
probability of less than 0.001
Questions
1
2
d mean
a There is no significant difference between the digit size for the two populations of birds.
Any difference is due to chance.
b
t  2.036
c
d
degrees of freedom  75
Any two of:
the probability is less than 0.05, reject the null hypothesis
there is a significant difference between the bird populations
the differences are not due to chance.
© Oxford University Press 2015
(1 mark)
(1 mark)
(2 marks)
(1 mark)
(1 mark)
(1 mark)
(1 mark)
www.oxfordsecondary.co.uk/acknowledgements
This resource sheet may have been changed from the original
2