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ACL Inclination Angle on MRI and Femoral Tunnel Angle on AP X-ray: A Comparison Study with Tunnel Positions on 3D CT in Single Bundle ACL Reconstruction. 1 Illingworth, KD, 1Hensler, D, 1Working, ZM, 2Casagranda, B, 1Tashman, S, +1Fu, FH +1University of Pittsburgh, Pittsburgh, PA. Department of Orthopaedic Surgery 2 University of Pittsburgh, Pittsburgh, PA. Department of Radiology [email protected] INTRODUCTION Anatomical placement of grafts during anterior cruciate ligament (ACL) reconstruction may lead to better control of rotational stability and help to preserve long term knee health. However, anatomical tunnel position can be difficult to validate from magnetic resonance imaging (MRI) or plane x-rays. The purpose of this study was to investigate whether the ACL inclination angle and femoral tunnel angle on clinically available 2D images can be used to evaluate anatomic positioning after single bundle (SB) ACL reconstruction. MATERIALS AND METHODS With IRB approval, 50 patients were identified that had SB ACL reconstructions and post-operative AP x-ray, MRI with intact ACL graft and CT scans. 50 patients that had native intact ACL on MRI were identified for a comparison group. Tunnel Position on 3D CT: The CTscans of each patient were imported into Mimics (Materialise, Leuven, Belgium) for segmentation and then Geomagic Studio (Geomagic, Research Triangle Park, North Carolina) for 3D model development and analysis. Tunnel positions of single bundle reconstructions were analyzed on both the femur (figure 1a) and tibia (figure 1b) using anatomical coordinate systems and normalized to the AP and ML dimensions of the tibia or femur, as described by Forsythe et al [1]. and our approximation of the long axis of the femur is used for femoral tunnel angle measurement as shown in fig 3b. Figure 3- X-ray Femoral Tunnel Angle RESULTS The native ACL inclination angle average was 49.9° ± 2.8°, with a range from 43-57°. ACL inclination angle on MRI for SB reconstructions ranged from 46.4° to 79.4° and femoral tunnel angle ranged from 2.1° to 46.1°. There was a strong correlation between anterior tunnel position on the femur and an increase in ACL MRI inclination angle (p<0.001, r = 0.74) and decrease in x-ray femoral tunnel angle (p<0.001, r = 0.78), as well as a good correlation between ACL inclination angle and femoral tunnel angle (p<0.001, r = 0.63). Figure 1- CT Anatomic Coordinate System and tunnel position. ACL Inclination angle on MRI: A single sagittal proton density image best showing the ACL was selected. An estimation of the long axis of the tibia was determined by selecting the most distal portion of the tibia and measuring the width of the diaphysis (line a) on a line parallel to the physeal scar. Another line was measured proximal on the diaphysis half the distance of the most distal line measured. The midpoint of both lines was determined and a line bisecting these points was drawn. The tibial horizontal line (THL) was drawn perpendicular to this line at the most distal portion of the ACL. The angle between a line drawn parallel to the anterior most fibers of the ACL (Line d) and the THL was designated as the ACL inclination angle. Figure 2- ACL Inclination Angle Femoral Tunnel Angle on AP radiographs: A line is drawn from the most proximal points of the femoral epidondyles, which we refer to as the trans-epicondylar axis (DTE), as shown in figure 3a. Next, the most proximal portion of the femur is located and the width of the diaphysis is measured in a parallel fashion to the DTE. Another width of the diaphysis is measured distally half the distance of the DTE. The midpoint of both line a and line b is determined. A line is drawn down the center of the femoral shaft connecting the midpoints. An axis bisecting the femoral tunnel is drawn and the angle between this axis DISCUSSION Forsythe et al. described the normalized femoral footprint of the native ACL to be between 0.089 and 0.364, from posterior to anterior [1]. The SB reconstructions that fell within this range had an MRI inclination angle range of 46.4° - 54.8°, which is consistent with the native MRI inclination angles measured in this study. Also, these same reconstructions had an x-ray femoral tunnel angle range of 33.9° - 46.1°. Although it is possible to have a non-anatomic SB ACL reconstruction that falls within these ranges of MRI inclination and femoral tunnel xray angle, these simple measurements on routine clinical imaging allow the surgeon to have a quick an easy measurement that can aid in determining whether a graft is placed anatomically. 1. Forsythe et al. (2010). J Bone Joint Surg Am Poster No. 871 • ORS 2011 Annual Meeting