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Specialized Plant Cells: Transport in Leaves 9 Bio Leaf Structure- Overview • Leaf structure is arranged in layers: – 1. Upper epidermis • Waxy cuticle prevents water loss – 2. Pallisade layer • Chloroplasts – photosynthesis (glucose production) – 3. Spongy pallisade layer • Storage of H2O, CO2 and O2 – 4. Lower epidermis • Stomata and guard cells regulate the passage of water vapor( transpiration), intake of CO2, output of O2 Palisade Mesophyll Layer • Contains chloroplasts: – Outer membrane – Granum- stack of thylakoids – Thylakoids- contain chlorophyll, catch photons of light photosynthesis glucose – Stroma- fluid filled space Spongy Mesophyll Layer • Open space to store gases ( CO2 and O2) and H2O molecules for exchange in atmosphere – CO2 needed for photosynthesis – O2- by-product of photosynthesis – H2O- transpiration Lower Epidermis • Has guard cells surrounding stomata – Stomate (singular) – Opening and closing of stomata regulated by plant hormone abscisic acid • Abscisic acid present: K+ leaves, H2O leaves, stomata close • Abscisic acid absent: K+ stays, H2O stays, stomata open Regulation of Transport • Lower Epidermis: – Takes in CO2 for photosynthesis – Releases O2 from photosynthesis – Releases H2O (Transpiration) • Why would stomata remain closed in arid conditions? • When might plants lose the most H2O? • When might plants produce a high level of O2? • When might plants need a greater amount of CO2?