Download Clinical Group

Clinical Group
 Biology
of HIV infection
 by Duangrat Inthorn
 Mechanism of Reverse Transcriptase
Inhibitors and Protease Inhibitors
 by Tawitch Suriyo
 Animal Models for HIV
 by Pornpat Intarasunanont
Clinical Group (continue)
 Clinical
Trails- Crixivan
 by Somjed Sahasitiwat
 Clinical Trails-Drug Interactions and
Side Effects
 by Peerakarn Banjerdkij
 Combination Therapy and Resistance
 by Ormrat Kampeerawipakorn
Clinical Group (continue)
 Targeting
of the CD4 Binding Site
by Tan Swee Huang
 Gene Therapy
 by Pairoaj Vonghathaipaisarn
 DNA vaccines
 by Zhang Jianjun
The Biology of HIV Infection
Asst. Prof. Dr. Duangrat Inthorn
Dept. of Environmental Health
Science, Faculty of Public
Health, Mahidol University
Scope
 Biology
of HIV , structure of HIV,
genome of HIV, life cycle of HIV
 HIV -1 and related Retrovirus
 How it might be stopped (Drug
development)
Introduction
The virus that cause AIDS (Acquired
Immunodeficiency Syndrome, AIDS) is HIV
(Human Immunodeficiency Virus, HIV)
Homosexual have Pneumocyatis carnii
together with herpes simplex, cytomegalovirus
and Candida
Kaposi’s sarcoma- KS is a few case in
healthy person
-Outer lipid bilayer coat
studded with surface
(SU, gp 120)
-transmembrane (TM,
gp41) glycoprotein
complexs
-beneath the lipid bilayer
are matrix proteins (MA)
-the virion consist of
internal capsular (CA) and
nuclear capsid (NC) proteins
which surround the single stranded RNA genome.
Structure of HIV
- show the
components and
their genetic source
-the matrix (MA),
internal capsular
(CA), and nuclear
capsid (NC) proteins
-produce by the gag
region
-contain gene
products of the pol
region- RT, integrase
and protease.
HIV virion
The Genome of HIV
 HIV
genome is 9749 nucleotides about
the same size as any other retrovirus,
for example Rous sarcoma virus (RSV)
 But genome of HIV is more complex
than RSV since it has extra open
reading frames that clearly code for
small proteins.
The Genome of HIV
 The
HIV genome has nine open
reading frames but 15 proteins are
made in all
 The GAG/POL polyprotein and the
ENV polyprotein are cleaved into
several proteins:
 GAG is cleaved to: MA (matrix),
CA(capsid), NC (nucleocapsid), p6
 POL is
cleaved to: PR(protease),
RT(reverse transcriptase), IN
(integrase)
 ENV is cleaved to : SU (gp120) and
(gp41)
 Of other proteins, three are
incorporated into the virus (Vif, Vpr
and Nef) while three are not found in
the mature virus: Tat and rev are
regulatory proteins and Vpu indirectly
assists in assembly
TAT : Trans-Activator of Transcription
REV: Regulator of Virion protein expression
NEF : Negative Regulatory Factor
VIF : Virion Infectivity Factor
VPU : Viral Protein U
VPR : Viral Protein R
These genes encode small proteins. They
overlap with the structural genes (especially
ENV) but are different reading frames.