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BIOL 362: Cellular Physiology
Course Outline and Introduction (2011)
Biology 362: Cellular Physiology Overview:
From the membrane to the nucleus to the extracellular matrix – this is a course based on the spatial
organization of critical events in the life of eukaryotic cells, with examples from plants, fungal and
animal systems.
Biology 362 is a 2-credit lecture course in Cellular Physiology. The labs formerly associated with this
course are now given as a freestanding lab course (BIOL 360). Biology 362 concentrates on cell
biology and physiology during critical events including division, morphogenesis and motility. The
course explores the mechanisms cells use to integrate and communicate signals and emphasizes the
cytoskeleton and its role in cellular dynamics and the regulation of cell growth and physiology. In
particular, research from experimental model systems will be emphasized throughout to illustrate basic
principles in cell biology and physiology.
Learning Goals:
1) to obtain an understanding of concepts, processes, and experimental approaches related to cell
biology and physiology, from the extracellular matrix to the nucleus.
2) to understand how the scientific process can be used to answer questions concerning the
regulation of cell growth, division and general physiology. You will be asked to use specific
experimental approaches to answer questions and test hypotheses and interpret new data in light
of your understanding of concepts and processes.
3) to appreciate the practical importance of the cytoskeleton, cell dynamics and the regulation of
cell growth, division and physiology. Thus, in addition to learning what is known about
cytoskeleton and the regulation of cell growth and development, you will be asked to learn how
this is known and to use this information to solve problems.
Prerequisite(s):
Either (a) BIOL 361 or (b) all of BIOL 201, BIOL 351.
We will use clickers in our classes. Please ensure that you register your PRS number on Vista so
that it is synchronized with your student number.
Required Reading:
Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter.
MOLECULAR BIOLOGY OF THE CELL, Garland Science, Fifth Edition (2008), ISBN 978-08153-4105-5 or 978-0-8153-4106-2. (Referred to as MBoC for brevity)
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Appropriate reading assignments from the text will be given regularly:
Chapter 8: Manipulating proteins, DNA, and RNA
Chapter 9: Visualizing Cells
Chapter 15: Mechanism of Cell Communication
Chapter 16: The Cytoskeleton
Chapter 17: The Cell Cycle
Chapter 19: Cell Junctions, Cell Adhesion, and the Extracellular Matrix
Recommended Reading:
John Wilson and Tim Hunt. MOLECULAR BIOLOGY OF THE CELL: The Problems Book, Garland
Science, Fifth Edition (2008), ISBN 978-0-8153-4110-9. (Referred to as MBoC-PB for brevity)
Copies of this problems book are available for purchase in the UBC Bookstore. We will be selecting
problems for our tutorials from this book so it is recommended but not mandatory.
Supplementary Reference Material:
Lessons PowerPoints, selected articles, book chapters, and other material will be posted on the Vista
Website (http://www.vista.ubc.ca (you will need your CWL i.d. and password to access the course
website).
Course Evaluation
In-class clicker quizzes
10%
Test #1 (Feb. 1)
13%
Test #2 (Mar. 1)
13%
Test #3 (Mar. 22)
14%
Final Exam
50%
*Tests cover all lessons since the last
test
Exam Absence or Deferral
For quizzes, absence due to a scheduling conflict (ex. religious holiday) must be discussed with the
instructor at least 3 weeks prior to the planned absence. Absence due to unforeseen circumstances (e.g.
illness) must be verified by a medical practitioner’s certificate. In either case, the points will be
assigned to the in-class or final exam. For the final exam, students must provide two weeks notice to
the Science Information Centre of a foreseen absence, or contact the Science Information Centre within
48 hours of the missed exam in case of emotional or medical difficulties
(http://www.science.ubc.ca/students/advising/office).
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Course Schedule
Biology 362 will have two lectures per week scheduled within a 1.5h classroom time (Tuesday and
Thursday 11:00am to 12:30pm , West Mall Swing Space Rm122.), with tutorials included in the
normal lecture times. Tutorials, based on problem sets posted online will be used to develop your
ability to critically evaluate experimental evidence related to the concepts covered in the lectures, or to
understand techniques.
Module
Topic
Day
Date
2011 Lesson
Course introduction
Investigative Research methods for cellular
Techniques
physiology
General principals in signaling
Signaling through G-protein-coupled
receptors
Signaling through enzyme-coupled
surface receptors
Cell-cell
Signaling through small intracellular
communication mediators
Signaling pathways that depend on
regulated proteolysis
Signaling in plants
Test #1 (20 min.), Cumulative review
session
Assembly mechanisms
Nucleation
Accessory proteins
Reading week – no class
Cytoskeleton Reading week – no class
Drugs and herbicides
Motor proteins
Test #2 (20 min.),, Cumulative review
session
Motile processes and cell behaviour
Motility
Cell polarity and motile behaviour
Cell cycle intro
Mechanics of Mitosis
Cell cycle
Cytokinesis? cut from 2009
Test #3 (20 min.), Cumulative review
session
Cell-cell adhesion - intro
Tight junctions and channel-forming
Cell-cell
junctions
interactions
Extracellular matrix of animals
Plant cell walls
Cumulative review session
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Tuesday
04-Jan-11 Lesson 01
Thursday
Tuesday
06-Jan-11 Lesson 02
11-Jan-11 Lesson 03
Thursday
13-Jan-11 Lesson 04
Tuesday
18-Jan-11 Lesson 05
Thursday
20-Jan-11 Lesson 06
Tuesday
Thursday
25-Jan-11 Lesson 07
27-Jan-11 Lesson 08
Tuesday
Thursday
Tuesday
Thursday
Tuesday
Thursday
Tuesday
Thursday
01-Feb-11
03-Feb-11
08-Feb-11
10-Feb-11
15-Feb-11
17-Feb-11
22-Feb-11
24-Feb-11
Test # 01
Lesson 09
Lesson 10
Lesson 11
Reading Week
Reading Week
Lesson 12
Lesson 13
Tuesday
Thursday
Tuesday
Thursday
Tuesday
Thursday
01-Mar-11
03-Mar-11
08-Mar-11
10-Mar-11
15-Mar-11
17-Mar-11
Test #02
Lesson 14
Lesson 15
Lesson 16
Lesson 17
Lesson 18
Tuesday 22-Mar-11 Test #03
Thursday 24-Mar-11 Lesson 19
Tuesday 29-Mar-11 Lesson 20
Thursday 31-Mar-11 Lesson 21
Tuesday 05-Apr-11 Lesson 22
Thursday 07-Apr-11 Review
Instructor:
Hardy Hall
Department of Botany, The University of British Columbia
Brian Ellis Laboratory
Office: Room 385, Michael Smith Laboratories
#301-2185 East Mall
Phone: 604 827 4550
Email: [email protected]
Office hours: Thursdays 2:00pm to 4:00pm
Hardy Hall is a Ph.D. student currently writing his thesis on molecular phenotyping of cell wall
expansion in the Arabidopsis thaliana model system. This work is being conducted in the Michael
Smith Laboratories under the supervision of Prof. Brian Ellis. His curriculum vitae may be viewed at
www.plantometrics.com.
Course Designer:
Geoffrey Wasteneys
Department of Botany
On sabbatical
Dr. Wasteneys is a Professor in the Department of Botany and a Tier 1 Canada Research Chair in
Plant Cell Biology. He has a BSc from Carleton University (1983), a PhD in Plant Cell Biology
from the Australian National University (1989), was a Humboldt Fellow at the University of
Heidelberg (1992-93), and a Queen Elizabeth II Fellow at the Australian National University
(1992-98), where he became a Faculty member at the Research School of Biological Sciences. He
moved to UBC in 2004, and directs research on the cytoskeleton and cell walls using Arabidopsis
thaliana as a model system. Dr. Wasteneys is the Academic Director of the UBC Bio-Imaging
Facility, is an Editor for The Plant Journal, Functional Plant Biology, a member of Faculty of
1000, and also teaches BIOL 352.
Dr. Wasteneys will be acting as a course consultant, monitoring progress throughout the term to ensure
students receive top quality instruction.
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