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The Effect of Potassium Nitrate and Cold Moist on Milkweed Seeds Introduction Chrissy Martinez and Christina Graber Merrill F. West FFA Division 3 Environmental Science Materials Milkweed plants are the main habitat for the 400 seeds of Asclepias Speciosa milkweed seeds, 1 monarch butterfly. Female monarch butterflies must tsp of potassium nitrate, 2 beakers, potting soil, 4 find milkweed to lay their eggs. Monarchs choose planting trays, refrigerator, zip-lock bags, paper to lay their eggs on milkweed is because most towels, hot water, 1 plastic spoon, 4 labels, and cold milkweed plants contain cardiac glycosides. When water. caterpillars consume the leaves of the plant their Procedure tissues become toxic to predators. This protection continues throughout their entire life. In this experiment 2 trials were completed. In both trials the scarification procedure included Newly hatched monarch caterpillars consuming milkweed. soaking the seeds in a potassium nitrate solution for 24 hours. In trial 1 the seeds went through a two month stratification in a refrigerator prior to Need for Study planting and for trial 2 the seeds went through a Planting the milkweed seeds seeds for trial 1. Monarch butterflies are in danger because of the loss of milkweed plants due to urbanization. landowners to restore milkweed on their properties Research Questions Number of Seeds Germinated break the seeds dormancy. Conclusion 40 through a more efficient methods of propagating. scarification and stratification process in order to planting. Trial 1 Results Improving methods of germination will allow This experiment will imitate nature’s natural three month stratification treatment prior to 35 Trial 1 Germination results Scarification and 60 day stratification . The hypothesis was partially supported. The 30 25 potassium nitrate scarification and cold moist 20 15 stratification method combined did not perform as 10 well as predicted. The potassium nitrate 5 scarification alone had the most outstanding 0 0 Days 6 Days 10 Days 16 Days 22 Days Days Collected Data Control Cold Moist KNO 3 Cold Moist & KNO 3 Will potassium nitrate and cold moist reduce the dormancy rate of milkweed seeds? What is the Figure 1 Milkweed germination germination rate of the seeds tested and not tested Hypothesis If milkweed seeds are treated with potassium nitrate and cold moist then it will improve the germination rate in the seeds. Trial 2 Germination results Scarification and 90 day stratification Number of Seeds Germinated products separately? of the potassium nitrate/cold moist group. Future procedure. 60 cold moist solution and potassium nitrate together increase germination rate faster than using the two germination rate and it decreased the germination studies will include a revised stratification Trial 2 Results with potassium nitrate and cold moist? Will using performance. The cold moist had the lowest Works Cited 50 40 30 Brianna Borders, E. L.-M. (n.d.). Milkweed sA 20 Conservation Practitioner's Guide. The Xerces 10 Society for Invertebrate Conservation. Gluesenkamp, D. (2015). Milkweeds. Retrieved 0 0 Days 6 Days 8 Days 9 Days 12 Days 16 Days Days Collected Data Control Cold Moist KNO 3 Cold Moist & KNO 3 Figure 2 Milkweed germination from California Native Plant Association: http://www.cnps.org