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C383 Study Guide for Exam 1
Spring 2017
Basic Information
 Exam 1: Thursday, February 9, 7:15-9:15PM in Ballantine 013
 Arrive early for assigned seats
 Bring your student ID. Failure to do so will result in getting your exam back later and
could result in a point penalty.
 You may use a NON-PROGRAMMABLE calculator.
 All papers, books, phones, and electronic devices must be in a sealed bag under your seat.
Exam Content: The exam will cover chapters 1-6 and 33. All material covered in class, Reading
Guides, book problems, and discussion sections could be on the exam. Some major topics
include:
Intermolecular forces, solvation, hydrophobic effect, auto-ionization, pH, pKa, pH of strong acid
solutions, weak acids, Henderson-Hasselbalch, ionization state, titration curves, buffer capacity,
polyprotic acids, blood buffer, nucleobase structures, nucleoside, nucleotide, polynucleotide
nomenclature, nucleases, base pairing, basis of double helix stability, denaturation and melting,
semiconservative replication, bacterial DNA structures, chromatin, RNA
structure/stability/function, amino acid structures: stereochemistry and ionization state, essential
amino acids, peptide bonds, disulfide bonds, structural effects of amide resonance, -helix,
Ramachandran plot, helical wheel, -sheets, loops and turns, motifs, domains, examples of
fibrous and globular proteins, thermodynamics of protein folding, salting-out proteins, size
exclusion chromatography, ion-exchange chromatography, affinity chromatography, SDSPAGE, isoelectric focusing, quantitation of protein purification, Edman Degradation, partial
digestion, enzyme rate enhancement, enzyme specificity, recognizing 6 classes of enzyme
catalyzed reactions, free energy, standard free energy, spontaneous and non-spontaneous
reactions, thermodynamics vs. kinetics, binding energy, proximity effect, induced fit, transition
state binding, transition state analogs
Memorize: amino acid structures, names, one- and three-letter abbreviations
Exam Format: The exam is out of 100 points. The first 60 points will be multiple choice, short
answer, fill in the blank type questions. There will be four 10-point problems involving
calculations, structural drawings, or short essay. There will be qualitative and quantitative
problems. Thermodynamic equations and constants will be given. There may or may not be a
bonus problem.
See practice exams posted on the website for format and practice.