Download 58548-AT-dapmag

ROBUST STUDY SUMMARY - Inherent Toxicity
Item
Yes
No
Reference: LeBlanc, G. A., and Cochrane, B. J. 1985. Modulation of substrate-specific glutathione stransferase activity in Daphnia magna with concomitant effects of toxicity tolerance. Comp. Biochem.
Physiol. 82C: 37-42.
Test Substance: CAS # and name (specify, but do not assess this item): 58-54-8 Ethacrynic acid
* Substance purity reported? (Y/N and specify)
X
Persistence/stability of test substance in aquatic solution reported? (Y/N)
X
Method
References (Y/N)
*OECD, EU, national, or other standard method? (Y/N)
If not a standard method, justification of the method/protocol provided? (Y/N)
*GLP (Good Laboratory Practice) (Y/N)
X
X
n/a
n/a
Test organisms (specify common and/or Latin names) Cladocerans Daphnia magna and Ceriodaphnia reticulata
Latin or both Latin and common names reported? (Y/N)
X
Life cycle age / stage of test organism (Y/N)
X
Sex (Y/N)
n/a
Length and/or weight of test organisms (Y/N)
n/a
Number of test organisms per replicate (Y/N)
X
Food type / feeding periods (acclimation/during test) (Y/N)
X
Test design / conditions
Test type – acute or chronic (specify, but do not assess this item): acute
Experiment type (laboratory or field) specified? (Y/N)
System type (static, semi-static, flow-through) (Y/N)
Negative or positive controls? (Y/N and specify) Negative
Number of replicates (including controls) and concentrations (Y/N)
Exposure pathways (food, water, both) (Y/N)
Exposure duration (Y/N and specify)
*Measured concentrations reported? (Y/N)
Exposure media conditions (temperature, pH, electrical conductivity, hardness, TOC,
DOC, DO, major cations and anions; other) (Y/N)
Was pH within 6-9 range? (do not assess this item) Yes
Was temperature within 5-28 °C range? (do not assess this item) Yes
Photoperiod and light intensity (Y/N)
Stock and test solution preparation (Y/N)
Information on emulsifiers used for poorly soluble / unstable substances (Y/N)
Analytical monitoring intervals (Y/N)
Statistical methods used (Y/N)
X
X
X
X
X
X
X
X
X
X
X
X
X
Results
Toxicity endpoints / values / units (specify, but do not assess this item):
Other endpoints reported (e.g. BCF/BAF, LOEC/NOEC, etc.) (specify, but do not assess this item):
*Was toxicity value below the chemical’s water solubility? (Y/N)
n/a
Other adverse effects (carcinogenicity, mutagenicity, etc. Do not assess this item)
Score: major items - 1/4 (25%);
overall score: 13/20 = 65%
EC Reliability code: 3
Reliability category (high, satisfactory, low): Low
Comments: Ethacrynic acid is a pharmaceutical drug used to treat high blood pressure and fluid retention in
people. The report was to determine the effects upon glutathione s-transferase (GST) production by cladocerans
in the presence of ethacrynic acid. No toxicity values were determined.