Download IMMUNOLOGY LAB PRACTICAL

IMMUNOLOGY LAB PRACTICAL: STUDY GUIDE
For each of the laboratory exercises, you should be able to do the following:
 State objectives
 Understand the theories involved
 Know how to do the methods
 Understand the rationale of the experimental design, including controls
 Know the study questions for each lab
I.
II.
III.
IV.
V.
VI.
VII.
White Blood Cell Study: Diff Quik method
 neutrophils, lymphocytes, monocytes, eosinophils, basophils
 identify microscopically
 know functions
 normal percentage in blood
 interpretation of results
Antigen Preparation
 Bacteria used
 Preparation of cells
 harvest methods
 centrifugation
 instrument
 theory
 Sonication
 epitopes
 preservation
Titrations: determining a titer
 saponin
 srbc preparations: indicators
 microtiter equipment
 interpretation of results
 experimental design
Immunodiffusion
 theory
 equipment used
 stains
 designing patterns
 interpretations
Complement
 theory
 materials and methods
 interpretations
ELISA
 theory
 materials and methods
 controls
 interpretations
Serum electrophoresis: separation and quantification
 theories
 fraction identification and separation pattern
 instrumentation
 interpretations