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3/5 Daily Catalyst Pg. 89 Review
 1. In CR, NADH and FADH2 are electron shuttles that
deliver electrons. How much ATP is produced from
NADH and FADH2?
 2. What is the effect(s) of electrons NOT passing from
complex to complex?
 3. In PS, cyclic phosphorylation produces ________.
3/5 Daily Catalyst Pg. 89 Review
 1. In CR, NADH and FADH2 are electron shuttles that deliver
electrons. How much ATP is produced from NADH and FADH2?
 FADH2= 2 ATP
 NADH= 3 ATP
 2. What is the effect(s) of electrons NOT passing from complex
to complex?
 Water will NOT be made
 Hydrogen's will not be pumped across the membrane
 ATP Synthase will not produce ATP
 3. In PS, cyclic phosphorylation produces ________.
 ATP
3/5 Fun Fact
3/5 Class Business
 I will be gone Monday
 Sub packet due Wednesday
 Test corrections due Wednesday
 Key on the website and posted on the board
 I will not see you Tuesday
 On Wednesday, we will begin Chemistry!
 Have your sub packet and test corrections ready to turn in!
3/5 Agenda
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Daily Catalyst
Class Business
Enzyme lab
Review Quiz #22
Review
 Enzyme Lab
 When you finish the lab, work on the post-lab and
conclusion.
 Review quiz #22
CR REVIEW
Glycolysis
CAC
ETC
Fermentation
Location
Cytoplasm
Matrix of the
mito
Inner
membrane of
the mito
Cytoplasm
Reactant
Glucose
Acetyl CoA
NADH &
FADH2
dropping off
e’s
Pryuvate
Product
2 Pyruvate
-
Water & ATP
Alcohol and
lactic acid,
NAD+
ATP?
2
2
~36
NO!
NADH?
2
6
use
NO!
FADH2?
0
2
use
NO!
CH2OH
H
H
HO H
HO
OH
H OH
H
Glycolysis
Citric
acid
cycle
Oxidative
phosphorylation
Glucose
ATP
1
Hexokinase
ADP
CH2OH
P
O H
H
H H
OH
HO
H OH
Glucose-6-phosphate
2
Phosphoglucoisomerase
CH2O
P
O
CH2OH
H HO
HO
H
HO H
Fructose-6-phosphate
3
ATP
Phosphofructokinase
ADP
P O CH2 O CH2 O
HO
H
OH
HO H
Fructose1, 6-bisphosphate
P
4
Aldolase
5
P
O CH2 Isomerase
C O
CH2OH
Dihydroxyacetone
phosphate
Figure 9.9 A
H
C O
CHOH
CH2 O P
Glyceraldehyde3-phosphate
2 ATP molecules are
invested.
Need money to
make money!
6
Triose phosphate
dehydrogenase
2 NAD+
2
2 Pi
NADH
+ 2 H+
2
P
O
C
O
CHOH
CH2 O P
1, 3-Bisphosphoglycerate
2 ADP
7
Phosphoglycerokinase
2 ATP
O–
2
C
CHOH
CH2 O P
3-Phosphoglycerate
8
Phosphoglyceromutase
O–
2
C
H
C
O
P
O
CH2OH
2-Phosphoglycerate
9
Enolase
2 H2 O
2
O–
C
O
C
O
P
CH2
Phosphoenolpyruvate
2 ADP
10
Pyruvate kinase
2 ATP
2
Figure 9.8 B
O–
C
O
C
O
CH3
Pyruvate
4 ATP molecules are
produced.
4- 2(energy
investment phase)=
2 ATP left!
A closer look at the citric acid cycle
Glycolysis
Citric
acid
cycle
Oxidative
phosphorylation
S
CoA
C
O
CH3
Acetyl CoA
 Key Point #4:
FADH2
 Coenzyme
 Electron shuttle
 Not as energetic
as NADH
CoA
O
NADH
+ H+
COO–
COO–
1
CH2
COO–
NAD+
8
CH
H2O
COO–
CH2
Oxaloacetate
HO
C
COO–
Figure 9.12
CH2
2
CH2
COO–
HO
C
SH
COO–
Malate
HO
Citrate
CH2
COO–
CO2
Citric
acid
cycle
7
3
NAD+
COO–
NADH
COO–
CH
Fumarate
HC
CoA
CH2
SH
6
CoA
COO–
FAD
COO–
CH2
CH2
COO–
C
O
S
CoA
Succinate
Pi
CoA
ATP
O
COO–
NAD+
GTP GDP Succinyl
ADP
C
4
CH2
5
CH2
FADH2
SH
+ H+
a-Ketoglutarate
CH2
COO–
Figure 9.12
CH
Isocitrate
COO–
H2O
COO–
HC
NADH
+ H+
CO2
 Chemiosmosis and the electron transport chain
Oxidative
phosphorylation.
electron transport
and chemiosmosis
Glycolysis
ATP
Inner
Mitochondrial
membrane
ATP
ATP
H+
H+
H+
Intermembrane
space
Protein complex
of electron
carners
Q
I
Inner
mitochondrial
membrane
Figure 9.15
IV
III
ATP
synthase
II
FADH2
NADH+
Mitochondrial
matrix
H+
Cyt c
NAD+
FAD+
2 H+ + 1/2 O2
H2O
ADP +
(Carrying electrons
from, food)
ATP
Pi
H+
Chemiosmosis
Electron transport chain
+
ATP
synthesis
powered by the flow
Electron transport and pumping of protons (H ),
+
+
which create an H gradient across the membrane Of H back across the membrane
Oxidative phosphorylation
2 ADP + 2
P1
2 ATP
O–
C O
Glucose
Glycolysis
C O
CH3
2 Pyruvate
2 NADH
2 NAD+
H
2 CO2
H
H C OH
C O
CH3
CH3
2 Ethanol
2 Acetaldehyde
(a) Alcohol fermentation
2 ADP + 2
Glucose
P1
2 ATP
Glycolysis
O–
C O
C O
O
2 NAD+
C O
H
C OH
CH3
2 Lactate
Figure 9.17
(b) Lactic acid fermentation
2 NADH
CH3
PS REVIEW
Light Reaction
Dark Reaction
Location
Thylakoid membrane
Stroma
Reactants
Photons, water
CO2, ATP, NADPH
Products
O2, NADPH, ATP
Glucose (6times)
NADPH?
YES
use
ATP?
YES
use
Glucose?
Not produced
produce
O2?
yes
-
Chlorophyll?
yes
nope
H+
H+
H+
H+
H+
H+
ADP
ATP
H+
e+
NADP
NADPH
e-
H+
H+
H
H
H+
H+
O
H+
H+
H+
e-
H+
O
H+
(2 H+ & ½ O2)
P
Phase 2: The Calvin Cycle
CO2
RuBP
NADPH
ATP
NADP+
ADP
P
ORGANIC
COMPOUND
Enzymes Review
3/6 Birthday fun!
Unit 8 Energy Test
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Name on your scantron
Date: 3/6
Class: 3
Quiz: Energy
 PLEASE USE PENCIL!
 Grab a calculator if needed
 Time: 60 minutes
 Noise: 0 (SILENT)
3/6 Class Business
 I will be gone Monday
 Sub packet due Wednesday
 Test corrections due Wednesday
 Key on the website and posted on the board
 I will not see you Tuesday
 On Wednesday, we will begin Chemistry!
 Have your sub packet and test corrections ready to turn in!