Download Technical Manual No. TM0473 Version 11232009

Human H4 Receptor Membrane Preparation
Technical Manual No. TM0473
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Version 11232009
Introduction ….…………………………………………………………………………….
Background ………..……………………………………………………………………….
References ……………………………………………………………………………….
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Introduction
Catalog Number：M00375
Cell Line Name: CHO-K1/H4/Gα15
Gene Synonyms: GPRv53, HH4R, GPCR105, AXOR35, SP9144
Expressed Gene: Genbank Accession Number NM_021624; no expressed tags
Host cell: CHO-K1
Quantity: 1 vial (1 ml per vial)
Protein Concentration: 1 mg/ml
Buffer: 50 mM HEPES, 5 mM MgCl2, 1 mM CaCl2 and 0.2% BSA, pH7.4
Application: Binding assay for H4 receptor
Storage: Store at -80°C
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Background:
Histamine receptor family consists of four members, namely, H1, H2, H3, and H4. The histamine H4 receptor is the
most recently identified G protein-coupled histamine receptor that binds to several neuroactive drugs, including
amitriptyline and clozapine. So far, H4 receptors have been found only on haematopoietic cells. This signifies its
important role as a drug target for the treatment of inflammatory diseases.
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References
Leurs R, Chazot PL, Shenton FC, Lim HD, de Esch IJ.Br. (2009) Molecular and biochemical
pharmacology of the histamine H4 receptor. J. Pharmacol. 2009 May; 157(1):14-23.
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Lim HD, van Rijn RM, Ling P, Bakker RA, Thurmond RL, Leurs R. (2005) Evaluation of histamine H1-,
H2-, and H3-receptor ligands at the human histamine H4 receptor: identification of 4-methylhistamine as
the first potent and selective H4 receptor agonist., J. Pharmacol. Exp. Ther. 2005 Sep; 314(3):1310-21.
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