Download Slides - Set #3

Wild Type Drosophila
Ommatidium
8 Photoreceptor cells (R1-R8)
4 Cone cells (secrete lens)
8 Pigment cells
Wild Type Eye Phenotype
Eyeless Mutant Phenotype
(hypomorphic allele)
Paired Domain Protein
DNA
Figure 1A
Gal4 Enhancer-Trap Method
LacZ
No Transcription of LacZ
Figure 1A
Gal4 Enhancer-Trap Method
LacZ
Enhancer that controls
expression in specific tissues
Figure 1A
Gal4 Enhancer-Trap Method
LacZ
Tissue-specific Expression
of Gal4
Tissue-specific Expression
of LacZ
Figure 1
Eye-Antennal disc
wing disc
leg disc
Figure 1A
Gal4 Enhancer-Trap Method
LacZ
Enhancer that controls
expression in imaginal
disc cells
Transcription of LacZ
in imginal disc cells
Figure 1A
Enhancer that controls
expression in imaginal
disc cells
Transcription of eyeless
in imginal disc cells
Figure 2
Figure 3
Paired Domain Protein
DNA
Drosophila ey, mouse Pax-6 (sey) and Human Pax-6 (Aniridia) genes
are ~ 94% similar in Paired domain
Ectopic Expression of Mouse Pax-6 Gene
Pax-6
Enhancer that controls
expression in imaginal
disc cells
Transcription of Pax-6
in imginal disc cells
Figure 5
Ectopic Expression of Mouse Pax-6 Gene
Aniridia -- Human Pax-6 Mutation
Absence of the iris
Xenopus Oocyte
Animal Pole
Vegetal Pole
Xwnt-11 mRNA Localization
Xwnt-11 is Drosophila wingless homogue
Ventralized
Normal
Embryo Grafting Experiment
Goosecoid
Protein
Expression
Embryo Grafting Experiment
Inject
mRNAs
For
Chordin etc.
Formation of ventral structures
Normal
Dorsalized