Download Types of liquid chromatography

7
King Abdulaziz University
Faculty of Science (girls)
Chemistry Department
third Exam. Second Semester
Course 313 CHEM
Time allowed: 1h
Date: 8/5/1429
Student name:
Student number:
Chose the correct answer:
1) Molecular sieve is:
A) Polymeric carbohydrates and acrylamides
B) Hydrocarbons
C) Resin
D) Silica and calcium carbonates
2) Mechanism of liquid-solid chromatography
A) Adsorption
B) Partion
C) Adsorption-partition
D) Affinity
3) Types of detector used in HPLC is
A) UV detector only
B) UV, RI, FID and fluorescence
C) FID only
D) No one of them
4) Rate of solutes distribution between stationary and mobile phase in liquid
chromatography is:
A) From High to low
B) From Low to high
C) high
D) Low
5) Gel with high degree of swelling used for fractionate
A) All molecular size
B) Large molecular size
C) Small molecular size
D) Medium size
6) Strong functional exchanger group in cation exchange resin is
A) Amine group
B) sulfonic acid
C) Quaternary ammonium ion
D) Carboxylic acid
7 ) Degree of cross-linking in Ion-exchange chromatography determined by
A) DVB only
B) Styrene only
C) Styrene- DVB
D) Cation- anion resin
8) Why we control pH at the separation amino acid by ion-exchange?
9) What are types of liquid chromatography?
10) What is exchange capacity of resin?
11) What the anion Exchange resin? What are functional exchanger group ?
12) Concept of size exclusion chromatography? What compounds can be separated by
Size exclusion chromatography?
13) Draw schematic diagram of hplc instrument? What are the most parts?