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Risk Assessments
List of Special Risk Assessments
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No.
Title
Keywords
456
TEM sample preparation
GLUTARALDEHYDE, PARAFORMALDEHYDE, OSMIUM TETROXIDE, POTASSIUM FERRICYANIDE, URANYL ACETATE,
ACROLEIN, TAAB LOW VISCOSITY RESIN, ACETONE
455
Development of wound dressing using
human skin
HUMAN SKIN TISSUE, methicillin-sensitive Staphylococcus aureus MSSA 476, Pseudomonas aeruginosa PAO1,
Escherichia coli DH3alpha
454
Intraperitoneal administration of mice
with Tamoxifen and subsequent animal
care
TAMOXIFEN, MICE
453
Growing algae on waste water
CO2, CARBON DIOXIDE, OZONE, WASTE WATER, SEWAGE
452
Monitoring Great Bustards in the UK
DRIVING, FIELDWORK, LONE WORKING, SALISBURY PLAIN, ADVERSE WEATHER, TICK-BORN DISEASES, LYME
DISEASE
451
Micromanipulation of mouse embryos
GLASS CAPILLARIES, MERCURY, CYTOCHALASIN B AND D, DMSO, BUTANE/PROPANE, CO2, CARBON DIOXIDE
450B Metabolism of sodium benzoate (and
substituted benzoates) by Alacligenes
eutrophus B9
ALCALIGENES EUTROPHUS, CENTRIFUGE
449
Spraying acetate sheets with oil mist
impregnated with spores of Metarhizium
anisopliae
METARHIZIUM ANISOPLIAE SPORES, OIL MIST, SHELLOMDINA EL, SHELLSOL T
448
Microdialysis probe implantation and
sample collection
ISOFLUORANE, LAB ANIMALS, SHARPS, DENTAL CEMENT, NICOTINIC DRUGS, DRY ICE, PARAFORMALDEHYDE,
EUTHATAL, ETHANOL
447
Anaesthetising Drosophila with gaseous
CO2
CARBON DIOXIDE, CO2
446
Using a ladder for nestbox installation and LADDER
observation
445
7-AAD staining of Drosophila embryos
7-AMINOACTINOMYCIN D
444
Collection (by venopuncture) and alaysis
of blood from human volunteers
UNSCREENED HUMAN BLOOD, BLOOD-BORNE VIRUSES, FAINTING
443
Working with Methylmethacrylate
monomer (Simplex Rapid Liquid)
METHYLMETHACRYLATE MONOMER, SIMPLEX RAPID LIQUID
No.
Title
442
In vitro cultivation of Borrelia species and BORRELIA SPECIES, AMMONIA
preparation of DNA
Keywords
441
RO dosing/flushing system in 4S 0.16G
aquarium
440
Use of various toxic screening compounds MATRIX METALLO PROTEASE INHIBITORS, DMSO
439
Cytochrome P450 inhibition by CO
CO, CARBON MONOXIDE
438
Curing of cultured cells "infected" with
PrPSc (abnormal prion protein)
SHEEP/MOUSE TUMOUR CELLS, PROTEINASE RESISTANT PRION PROTEIN, PrPres, PrPsc, SDS, NAOH
ROCIDE DB5, 2,2-DIBROMO-3-NITROPROPIONAMIDE, PRESSURE
437B Testing of neurotoxicity of peptides on
cultured cells
PRION OR DOPPEL PROTEIN SEQUENCES
436
Seed and plant mutagenesis with EMS
EMS, METHANE SULFONIC ACID ETHYL ESTER, NAOH, SODIUM HYDROXIDE
435
Immunocytochemistry (light microscopy)
PARAFORMALDEHYDE, DIAMINOBENZIDINE, DAB, METHANOL, CHLOROFORM, DPX, XYLENE, FORMALDEHYDE,
HYDROGEN PEROXIDE, GLACIAL ACETIC ACID, PICRIC ACID
434
Mutagenesis of C. elegans worms
METHANE SULPHONIC ACID ETHYL ESTER, EMS, ETHYL METHANESULPHONATE, 1,2:3,4-DIEPOXYBUTANE, 1,3BUTADIENE DIEPOXIDE, C. ELEGANS, NEMATODE WORMS
433
Preparation of acetamides from amines
ACETIC ANHYDRIDE, DICHLOROMETHANE, NAOH, SODIUM HYDROXIDE
432
Powered homogeniser in 1.37
LOOSE CLOTHING, LONG HAIR, BROKEN GLASS, AEROSOLS
431
Immunocytochemistry (light and electron ANIMAL TISSUE, LN, SODIUM AZIDE, DAB, DPX, ETHANOL, FORMALDEHYDE, ACETIC ACID, GLUTARALDEHYDE,
microscopy)
HYDROGEN PEROXIDE, OSMIUM TETROXIDE, PARAFORMALDEHYDE, PICRIC ACID, THIOMEROSAL, XYLENE
415
Production of fusion proteins by agarose
affinity columns
SONICATOR, LEUCOPEPTIN, PMSF, PHENYLMETHYL SULPHONYL FLUORIDE
414
Chromosomal fluorescent in situ
hybridisation (FISH)
UV, FORMAMIDE, METHANOL, GENTAMICIN, XYLENE, LIPOPOLYSACCHARIDE, DAPI, HCL
413
ENU mutagenesis of male Zebrafish
ENU, N-NITROSO-N-ETHYLUREA, HYPODERMIC NEEDLE, ZEBRAFISH
412
RNA preparation (LiCl/urea method)
ZEBRAFISH, LITHIUM CHLORIDE, PHENOL, CHLOROFORM, SDS, UREA, DEPC, ISOAMYL ALCOHOL, ISOPROPANOL
411
RNA isolation using TRI reagent
-70C, HUMAN CELLS, MOUSE CELLS, GM CELLS, PHENOL, MERCAPTOETHANOL, CHLOROFORM, GUANIDINE
THIOCYANATE, DEPC, ETHANOL, ISOPROPANOL
410
DNA preparation from animal tissue or
cultured cells
ANIMAL TISSUE, GM CELLS, CHLOROFORM, PROTEINASE K, ISOAMYL ALCOHOL, ISOPROPANOL, SDS, ETHANOL
409
Midi preps / mini preps / CaCl2
competent cell transformation
E. COLI, PHENOL, CHLOROFORM AMPICILLIN, TETRACYCLINE, SDS, ISOPROPANOL, SODIUM HYDROXIDE, ACETIC
ACID, ETHANOL
408
Minipreps (Miller & Miller method)
E.COLI, DODIUM HYDROXIDE, TRITON-X 100, DIETHYLPYROCARBONATE, ETHANOL
407
Non-radioactive in-situ hybridisation
PARAFORMALDEHYDE, METHANOL, FORMAMIDE, NITROBLUE TETRAZOLIUM, 5'BROMO-4-CHLORO-3-INDOYL
PHOSPHATE, DIMETHYLFORMAMIDE, FAST RED, TRIS, PROTEINASE K, ETHANOL
406
Non-radioactive RNA probe synthesis
-70C, PHENOL, CHLOROFORM, LITHIUM CHLORIDE, DIGOXIGENIN, GLYCEROL
405
Making DEPC water (RNase free water)
DIETHYL PYROCARBONATE
404
Making 4% paraformaldehyde in PBS
PARAFORMALDEHYDE, SODIUM HYDROXIDE
No.
Title
Keywords
403
Inhibition of melanin synthesis in
Zebrafish using PTU
1-PHENYL-2-THIOUREA
402
Whole-mount antibody staining/detection PARAFORMALDEHYDE, DIAMINOBENZIDINE, NICKEL CHLORIDE, TRITON-X 100, DIMETHYLSULPHOXIDE,
of biotinylated Dextran
HYDROGEN PEROXIDE
401
Tunel labelling to detect apoptotic cells
DIMETHYLFORMAMIDE, DIAMINOBENZIDINE, METHANOL, NICKEL CHLORIDE, FAST RED, PARAFORMALDEHYDE,
TRITON-X 100, DIMETHYLSULPHOXIDE, HYDROGEN PEROXIDE, SODIUM CACODYLATE, NBT, BCIP
326
Antibacterial activity of benzalkonium
chloride
BENZALKONIUM CHLORIDE, STAPHYLOCOCCUS AUREUS, SPAPHYLOCOCCUS EPIDERMIDIS
325
Growing Metahrizium flavoviride in
hydrocarbons
METAHRIZIUM FLAVOVIRIDE CONIDIA, 22 HYDROCARBON SOURCES
324
Peptide synthesis
PETROLEUM SPIRIT, METHANOL, DIMETHYLFORMAMIDE, DICHLOROMETHANE, TRIFLUOROACETIC ACID,
PIPERIDINE, 1-HYDROXYBENZOTRIAZOLE HYDRATE
323
Irradiating plasticware
GAMMA EMITTING SEALED RADIATION SOURCE
322
Extraction and identification of fungal
metabolites and enzymes
HPLC, A-NAPHTHOL,P-CRESOL, L-DOPA, ABTS, O-DIANISIDINE, HYDROGEN PEROXIDE, ETHYL ACETATE,
ACETRONITRILE, METHANOL
321
Use of metabolic inhibitors in experiments CTAB, 2,4-DNP, KCN, ACTINOMYCIN D, SODIUM ARSENATE, 4-HYDROXYCOUMARIN, ANTIMYCIN, 5-AZACYTIDINE,
on fungal development
PARAQUAT, POTASSIUM SUPEROXIDE, 1-PHENYL-2-THIOUREA, ROTENONE
320
Assays of enzyme and free radical
scavenging activity
ABTS, L-DOPA, O-DIANISIDINE, HYDROGEN PEROXIDE, DPPH, METHANOL, ACETONITRILE
319
Siliconisation of glassware
SIGMACOTE, HEPTANE
318
Phosphatidylinositol-3-kinase serinekinase assay
P32
317
GTP[alpha-P32] labelling of proteins in rat P32, ALPHA-TOXIN, SODIUM PERIODATE, SODIUM CYANOBOROHYDRIDE, SODIUM BOROHYDRIDE, WORTMANNIN
adipocytes
316
Measuring Trehalose by Anthrone
technique
THIOUREA, ANTHRONE, SULPHURIC ACID
315
Preparation and use of CAS assay
medium
CTAB, HEXADECYLTRIMETHYLAMMONIUM BROMIDE, PIPERAZINE, CHROME AZUROL, HYDROCHLORIC ACID
314
Transport assays
H3-GLUCOSE, TRITIATED GLUCOSE, SCINTILLANT, FORSKOLIN, CYTOCHALASIN B
313
Weigh out and dilution of berberine
hemisulphate
BERBERINE HEMISULPHATE
312
Making up a herbicide solution from
concentrate
ATRAZINE
311
Preparation of DNA from human blood
UNSCREENED HUMAN BLOOD, SDS, SODIUM DODECYL SULPHATE, PHENOL
310
Use of drugs that induce apoptosis
DOXORUBICIN, ADRIAMYCIN, ETOPOSIDE
309
Use of Biorad Biolistic PDS-1000/He
system
HIGH PRESSURE HELIUM GAS
No.
Title
Keywords
308
Preparative and analytical
chromatography
ACETONITRILE, METHANOL, ETHYL ACETATE, PETROLEUM ETHER
307
P13-kinase assay
PYRIDINE, P32 ATP, CDTA, BMT, 2,6,DI-TERT-BUTYL-METHYLPHENOL
306
Thiobarbituric acid assay
SODIUM ARSENITE, SODIUM PERIODATE
305
Tissue culture
ANIMAL CELLS, DEXAMETHASONE, PROGESTERONE, PUTRESCINE, ETHANOL
304
Preparation of Ergosterol standards
ERGOSTEROL, METHYLENE CHLORIDE, DICHLOROMETHANE, METHANOL
303
Extraction of Ergosterol from fungal
material
ETHANOL, DIETHYL ETHER, ERGOSTEROL
302
Purification of polyethylene glycol
DIETHYL ETHER, BENZENE, ISO-OCTANE, 2,2,4-TRIMETHYLPENTANE
301
Synthesis of hydroxyethyl polyvinyl
alcohol
ETHYLENE OXIDE, METHANOL
300
Crystallisation from nitromethane
BIS ANHYDRIDE OF FEROCENEDIOIC ACID, NITROMETHANE
299
Dilutions of Proxitone 0510 for biofilm
exposure experiment
PROXITANE 0510, PERACETIC ACID
298
Extraction and identification of fungal
metabolites and enzymes
METHANOL, CHLOROFORM, ETHYL ACETATE, ACETONTRILE, ALPHA-NAPHTHOL, P-CRESOL
297
Detection of sulphur : Sprayng TLC plates SODIUM AZIDE, RHODAMINE 6G, TRIPHENYL TETRAZOLIUM CHLORIDE
with indicator
296
Mutant screening - Specal media
UV, PRESSURE COOKER, UREA, SODIUM SELENATE, SODIUM CHLORATE, POTASSIUM CHROMATE, BENOMYL,
HYGROMYCIN B
295
Incubation of Cassava tuber discs and
radiolabelled amino acids
RADIATION, SHARPS
294
Treatment of 3T3-LI cells with tritiated
palmitic acid
TOLUENE, 3H, TRITIATED PALMITIC ACID
293
Making stock antibiotic solutions
AMPICILLIN, KANAMYCIN, CHLORAMPHENICOL, RIFAMPICIN, CYCLOHEXIMIDE, STREPTOMYCIN, GENTAMICIN
292
Solvent extraction of plant compounds
METHANOL, HEXANE, DIETHYL ETHER, CARBON DISULPHIDE
291
Pretreatment of 3T3LI adipocytes with
Pertussis toxin
PERTUSSIS TOXIN, INSULIN
290
Permeabilised 3T3LI transport assay with BENZALKONIUM CHLORIDE, INSULIN
benzalkonium chloride
289
Testing of lambda cyalothrin
288
Use of Tetanus toxin in permeabilised cell TETANUS TOXIN
transport assay
287
Use of Dustmaster DM1 respirator
DUSTS, INSECTS, LOCUSTS, MANDUCA
286
Making stock antibiotic solutions
AMPICILLIN, TETRACYCLINE, KANAMYCIN, CHLORAMPHENICOL
285
Use of -70'C freezer
-70'C, ULTRA COLD
284
Use of stacked disc centrifuge
HEAVY WEIGHTS (57 Kg), BACTERIA
LAMBDA CYALOTHRIN, PYRETHROID, ACETONE
No.
Title
Keywords
283
Electroporation
HIGH VOLTAGE ELECTRICITY (2.5 KV)
282
Use of U.V. radiation for visualising
nucleic acids
UV, ULTRAVIOLET RADIATION, ETBR, ETHIDIUM BROMIDE
281
RNA purification by guanidine thiocyanate GUANIDINE THIOCYANATE, PHENOL/CHLOROFORM/ISO AMYL ALCOHOL, CBS BUFFER, MERCAPTOETHANOL
disruption
280
Colourimetric dyes for immunological
methods
METHANOL, H2O2, HYDROGEN PEROXIDE, NITROBLUE TETRAZOLIUM, DMSO, DIMETHYL SULPHOXIDE, 3,3,5',5'
TETRAMETHYLBENZIDINE
279
Use of IPTG
IPTG, ISOPROPYL BETA-D-THIOGALACTOSIDE
278
Use of sodium azide as a preservative
SODIUM AZIDE
277
Use of hot plate stirrers and hot blocks
HEAT
276
Use of dry ice/ethanol cooling baths
CARDICE, SOLID CARBON DIOXIDE, DRY ICE, ETHANOL
275
Sonication of cells
SONICATION, ULTRASOUND, E. COLI, THERMOPHILES
274
Binding DNA to glass milk for purification
GUANIDINIUM THIOCYANATE
273
Use of PEG 6000
POLYETHYLENE GLYCOL 6000, PEG 6000
272
Tangential filtering
PRESSURE
271
Waste disposal
WASTE, SHARPS
270
Purif. of closed circular DNA by eqm.
cent. in CsCl-EtBr gradients
E. COLI, CAESIUM CHLORIDE, CsCl, ETHIDIUM BROMIDE, ETBR, ULTRACENTRIFUGE, CHLORAMPHENICOL, BUTAN1-OL
269
Dissection of Ascaris suum
ASCARIS SUUM, NEMATODE, HIGHLY ALLERGENIC BODY FLUID, LIQUID NITROGEN, LN
268
Weighing out of iodoacetic acid
IAA, IODOACETIC ACID, DMF, DIMETHYL FORMAMIDE
267
Plant enzyme extraction
ERWINIA AMYLOVORA, LIQUID NITROGEN, LN
266
Preparation of solutions of calcium
agonists and antagonists
LANTHANUM CL., COBALT CL., GADOLINIUM CL., TRIFLUOPERAZINE, VERAPAMIL, A23187 CA. IONOPHORE,
NIFEPIDINE, TMB-8, EGTA, NITRESPERDINE, FLUNARIZINE, DMSO, ETHANOL
265
Purification of alpha-SNAP and NSF from
E.coli cytosol
E.COLI, IMIDAZOLE, TRIS, GLYCEROL, PMSF
264
Growing E.coli and purifying alpha-SNAP
E.COLI, AMPICILLIN, HEPES, DTT, EDTA, PMSF
263
Electrophoresis of RNA through gels
containing formaldehyde
FORMALDEHYDE, FORMAMIDE
262
Isolation of micro-organisms from the
environment
PSEUDOMONADS, PSEUDOMONAS PUTIDA, RIFAMPICIN, ACETAMIDE, CHLOROACETAMIDE, MERCURIC CHLORIDE,
GLYPHOSATE, METHANOL
261
Coupling of peptide to carrier protein
MBS, M-MALEIMIDOBENZOIC ACID N-HYDROXYPURIMIMIDE ESTER, GLUTARALDEHYDE
260
Cryopreservation of plant tissue
DMSO,DIMETHYL SULPHOXIDE, ETHANEDIOL, GLYCEROL, SODIUM ALGINATE, LN, LIQUID NITROGEN, METHANOL
259
DIG DNA labelling and detection kit
DIGOXIGENIN-II DUTP (DIG), N,N-DIMETHYLFORMAMIDE (DMF)
258
Permeabilised 3T3-LI cell transport assay STREPTOLYSIN O, TETANUS TOXIN C FRAGMENT
257
Anatomical demonstartion of insect
tracheae using cobalt naphthenate
COBALT NAPHTHENATE, KEROSINE, PARAFFIN, SYRINGE, AMMONIUM SULPHIDE
No.
Title
256
Growing E. coli and purifying NSF - step 1 E. COLI, AMPICILLIN, PMSF, PHENYL METHYL SULPHONYL FLUORIDE, SONICATING
Keywords
255
Biotinylation of proteins (antibodies)
N-HYDROXYSUCCINIMIDE BIOTIN, DMSO, DIMETHYL SULPHOXIDE
254
Aldehyde oxidase purification and assay
ANIMAL TISSUE, HOMOGENISER, CENTRIFUGE, EDTA, CYSTEINE, DIMETHYLAMINOCINNAMALDEHYDE,
BENZAMIDINE
253
Polyclonal antibody purification from
blood by immunoaffinity chrom.
DIETHYLAMINE, TRIETHYLAMINE
252
Agarose gel electrophoresis
AGAROSE, MICROWAVING, HV ELECTRICITY
251
Staining gels in ethidium bromide
ETBR, ETHIDIUM BROMIDE
250
Preparation of ethidium bromide solutions ETBR, ETHIDIUM BROMIDE
249
Preparation of TRIS and TRIS based
buffers
TRIS (HYDROXYMETHYL) METHYLAMINE, HYDROCHLORIC ACID, ACETIC ACID, SODIUM HYDROXIDE, EDTA,
ETHYLENEDIAMINE TETRAACETIC ACID, BORIC ACID
248
DEPC (diethyl pyrocarbonate) treatment
of solutions
DEPC, DIETHYL PYROCARBONATE
247
Phenol:chloroform extraction of nucleic
acids
PHENOL, CHLOROFORM
246
Preparation of total RNA
GUANIDINIUM ISOTHIOCYANATE, SARCOSYL, BETA-MERCAPTOETHANOL, CENTRIFUGATION, PHENOL,
CHLOROFORM, ETHANOL
245
Freezing tissue in liquid nitrogen
LIQUID NITROGEN, LN, RAT
244
Preparation of polystyrene conjugates
EPICHLORHYDRIN, 1,4-BUTANEDIOL DIGLYCIDAL ETHER, SODIUM BOROHYDRIDE, TEREPHTHALALDEHYDE,
SODIUM AZIDE
243
Preparation of pentafluorobenzyl esters
PENTAFLUOROBENZYL BROMIDE, ALPHA-BROMOPENTAFLUOROTOLUENE, PFBB, N-ETHYL PIPERIDINE, ACETONE
242
Raising antisera to various antigens using HUNTER'S TITERMAX ADJUVANT, RABBITS, ANIMALS, SHARPS
Hunter's TiterMax adjuvant
241
Visualisation of phenolics on TLC plates
VANILLIN, ETHANOL, SULPHURIC ACID
240
Dye exclusion tests on plant cells using
Evans Blue & Phenosaffranine
EVANS BLUE, PHENOSAFFRANINE
239
Silver staining polyacrylamide gels
FORMALDEHYDE, SILVER NITRATE, TUNGSTOSILICIC ACID
238
Preparation of NSF protein
DTT, DITHIOTHREITOL
237
3-D structure determination of
STAPHYLOCOCCAL ENTEROTOXIN
enterotoxins from Staphylococcus aureus
236
Surface sterilisation using Mercuric
Chloride
MERCURIC CHLORIDE
235
Making buffer solutions
HCl, METHANOL, PROPAN-2-OL, DIMETHYLDICHLOROSILANE, SODIUM AZIDE, ACETONE, ACRYLAMIDE, SODIUM
CACODYLATE, ACETIC ACID, IMIDAZOLE, SODIUM FLUORIDE, POTASSIUM FLUORIDE
234
Culture and handling of insects : Locusts
LOCUSTS, SCHISTOCERCA GREGARIA, LOCUSTA MIGRATORIA
233
Immunohistochemical staining using
Diaminobenzidine
3,3-DIAMINOBENZIDINE TETRAHYDROCHLORIDE, METHYL SALICYLATE, HYDROGEN PEROXIDE,
PARAFORMALDEHYDE
No.
Title
Keywords
232
Plant chromosome fixing and staining
1-BROMONAPHTHALENE, 8-HYDROXYQUINOLINE, FEULGEN STAIN
231
Peracetic acid assay
TETRACHLOROETHYLENE, POTASSIUM IODIDE, SODIUM MOLYBDATE, SULPHURIC ACID, PERACETIC ACID
230
Preparation and use of diazomethane
DIAZALD (N-METHYL-N-NITROSOTOLUENE-P-SULPHONAMIDE), DIETHYL ETHER, ETHANOL, POTASSIUM
HYDROXIDE, DIAZOMETHANE
229
Extraction of destruxins
DESTRUXINS (FUNGAL TOXINS), CARBON TETRACHLORIDE, PROPAN-2-OL, ACETONITRILE
228
Mutagenesis using nitrosoguanidine
(NTG)
NTG, N-METHYL-N'-NITRO-N-NITROSOGUANIDINE
227
Pectin and starch analysis of plant
material
ETHANOL, SULPHURIC ACID, M-HYDROXYDIPHENYL, SODIUM HYDROXIDE, PERCHLORIC ACID, ANTHRONE, HEAT,
EDTA, SODIUM TETRABORATE
226
In situ hybridisation
POTASSIUM CACODYLATE, FORMAMIDE, 2-MERCAPTOETHANOL, GLUTARALDEHYDE, XYLENE, PERIODATE,
ETHANOL, ORGANOSILANE, PARAFORMALDEHYDE
225
Use of picric acid
PICRIC ACID
224
Staining of plant viral inclusions
AZURE A, CALCOMINE ORANGE 2RS, LUXOL BRILLIANT BL, 2-METHOXYETHANOL, CELLOSOLVE, EUPARAL
223
Chromosome staining plant roots or
stems (squash method)
SHARPS, QUINOLINE, ACETOALCOHOL, ACETIC ACID, ETHANOL, HYDROCHLORIC ACID, FEULGEN, BASIC FUCHSIN,
SODIUM METABISULPHITE, ORCEIN, ACETO-CARMINE, ACETIC ACID
222
Plant hormone extraction and purification METHANOL, BHT, BUTYLATED HYDROXYTOLUENE, IONISING RADIATION, DIETHYL ETHER, FORMIC ACID
221
Assay of succinate reductase
ETHYL ACETATE, TRICHLOROACETIC ACID, INT
220
Whole cell culture clamp
electrophysiology of Xenopus oocytes
DILUTE DRUG SOLUTIONS, ETHANOL, SHARPS
219
Microinjection of nucleic acid into
Xenopus oocytes
SODIUM HYDROXIDE, PENICILLIN, STREPTOMYCIN, GENTOMYCIN, MINERAL OIL, ETHANOL, DNA, SHARPS
218
Radioligand binding assays
NICOTINE BASE, ALPHA BUNGAROTOXIN, POLYETHYLENEINNINE, PEI, IONISING RADIATION
217
Preparation of brain membranes
(detergent extract)
PMSF, PHENYLMETHYL SULPHONYL FLUORIDE, SODIUM AZIDE, SODIUM HYDROXIDE, TRITON-X 100,
CENTRIFUGATION, RAT BRAIN
216
The use of mercaptoacetic acid in ()[3H]nicotine stocks
MERCAPTOACETIC ACID, IONISING RADIATION
215
Iodination of alpha-bungarotoxin
CHLORAMINE T, SODIUM METABISULPHITE, POTASSIUM IODIDE, ALPHA-BUNGAROTOXIN, I125
214
Synaptosome preparation by the Percoll
method
SHARPS, CENTRIFUGE
213
Perfusion
DILUTE DRUG SOLUTIONS. SHARPS, IONISING RADIATION
212
Immunocytochemistry
SODIUM CACODYLATE, TRITON-X 100, NITROBLUE TETRAZOLIUM, NBT
211
Choline acetyltransferase assay
NEOSTYGMINE, FORMIC ACID, HEPTAN-2-ONE, 3H-ACETYL CO A, TETRAPHENYLBORON, TRITON-X 100, OPTIPHASE
SAFE
210
TFA cleavage of peptide from resin
PHENOL, ETHANEDIOL, TFA, PETROLEUM ETHER
209
Transport assays - Inhibition with
tyrosine kinase inhibitors
TYROSINE KINASE INHIBITORS, 26C90, 201C88, ERBSTATIN, 453C89, 764C89, 690C88, 76W79, GENISTEIN
208
Part II Plant Pathology practical
No.
Title
Keywords
207
Histological tissue processing
FORMALIN, TBA, PHENOL, ETHANOL, TOLUIDINE BLUE, DPX, ACETIC ACID, XYLENE, RAZOR BLADES, MICROTOME
206
Use of DFP for inhibition of serine
proteases
DI-ISOPROPYL FLUOROPHOSPHATE, DFP
205
Fluorescence microscopy
UV RADIATION
204
Sterilisation of materials using liquid
sterilants
SODIUM HYPOCHLORITE, CHLOROS
203
Preparation of ethidium bromide stock
solution
ETHIDIUM BROMIDE
202
Fungal mutagenesis with NTG
NTG, N-METHYL-N'-NITRO-N-NITROSOGUANIDINE
201
Disruption of red blood cells by nitrogen
cavitation rel. malaria
HIGH PRESSURE NITROGEN, MALARIAL PARASITES, PLASMODIUM
200
Electrophoresis
ACRYLAMIDE, DIETHYL ETHER
199
Human fat cells
UNSCREENED HUMAN ADIPOSE TISSUE, BLOOD
198
Silver staining of polyacrylamide gels
OXIDISER, SILVER REAGENT, DEVELOPER
197
Calcofluor staining of biological material
CALCOFLUOR WHITE M2R NEW
196
Use of Teflubenzuron (Nomolt) against
desert locusts
TEFLUBENZURON, NOMOLT, LOCUSTS
195
Use of chromium trioxide as fixative
CHROMIUM TRIOXIDE
194
Effect of hydrogen peroxide on ciliates
H2O2, HYDROGEN PEROXIDE
193
Using SITS (4-acetamido-4'SITS, 4-ACETAMIDO-4'-ISOTHIOCYANATOSTILBENE-2,2'-DISULFONIC ACID
isothiocyanatostilbene-2.2'-disulfonic acid
192
Glutaraldehyde fixation of ciliates
GLUTARALDEHYDE
191
Aseptic cracking of oil palm seeds
70% ALCOHOL, CRACKING WITH VICE
190
Use of pressure bomb
PRESSURE BOMB, PRESSURES UP TO 300PSI
189
Fungicide (Sportak-alpha) application to
pollen
PENTAN-2-OL, SPORTAK-ALPHA, PROPAN-2-OL, DIETHYL ETHER, DIACETONE ALCOHOL, FUSARIUM SPORES, OILPALM POLLEN GRAINS
188
Solvent extraction for antifungal
substances in plants
PETROLEUM ETHER (60-80), PETROLEUM SPIRIT (60-80), DIETHYL ETHER, ETHYL ACETATE, METHANOL
187
Fungicide treatment of oil palm seeds
SPORETAK-ALPHA
186
Using 70% alcohol for surface disinfection ALCOHOL, ETHANOL
185
Use of domestic microwave oven
184
Column chromatography II
183
Blotting and hybridisation
FORMAMIDE, FORMALDEHYDE, SODIUM HYDROXIDE, NaOH, NITROCELLULOSE, RADIOISOTOPES
182
DNA sequencing
FORMAMIDE, DITHIOTHREITOL, TRIS-HCl, P32
181
Surface sterilisation using propylene
oxide
PROPYLENE OXIDE
MICROWAVE OVEN
No.
Title
Keywords
180
Cell wall isolation
PMSF, PHENYLMETHYLSULPHONYL FLUORIDE
179
Sugars analysis by DNS
3,5-DINITROSALICYLIC ACID, PHENOL, SODIUM SULPHITE, SODIUM HYDROXIDE
178
Glutathione assay
N-ETHYLMALEIMIDE
177
Modification of Destruxin and binding to a DESTRUXIN, AMMONIA .88 SOLUTION, PYRIDINE, SUCCINIC ANHYDRIDE, ACETONITRILE, DIMETHYLFORMAMIDE,
protein carrier
KEYHOLE LIMPET HAEMOCYANIN
176
Silver staining of Phast gels
GLUTARALDEHYDE, SILVER NITRATE, FORMALDEHYDE, ACETIC ACID
175
Acrylamide gel electrophoresis
ACRYLAMIDE, AMMONIUM PERSULPHATE, TEMED, RADIOISOTOPES
174
Agarose gel electrophoresis
AGAROSE, ETHIDIUM BROMIDE
173
E. coli fermentation
5 LITRES RECOMBINANT E. COLI
172
Cell wall extraction from plant tissue
CHLOROFORM, METHANOL, SPARK-FREE BLENDER, ACETONE
171
Enzyme extraction from plant tissue.
Stage 1
LN, LIQUID NITROGEN
170
Protein estimation
BIORAD, LOWRY, BCA, NaOH, SODIUM HYDROXIDE
169
Heavy metal soaking of protein crystals
URANYL ACETATE, URANYL SULPHATE, K3UO2F5, Pb(CH3C00)2, Na3IrCl6, K2PtCl4, K2PtCl6, K2PtCN4, NaAuCl4,
AuCN, Yb2(SO4)3, Ce2(SO4)3, SmCl3, La(CH3COO)3, Th(NO3)3, Hg(SCN)2, Hg(NO3)2, Hg(Cl2), PCHLOROMERCURIBENZOATE, P-CMB-SULPHONATE
168
Immunocytochemistry
PARAFORMALDEHYDE, GLUTARALDEHYDE, ACETONE, MERCAPTOETHANOL, COLLAGENASE, ETHANOL,
METHACRYLATE RESIN, UV MICROSCOPY, DIMETHYLANILINE, BENZOYL PEROXIDE
167
Extraction of insect peptides
PMSF, PHENYLMETHYLSULPHONYL FLUORIDE, ACETIC ACID, SULPHURIC ACID
166
Use of phenylthiourea in collecting insect
blood
PHENYLTHIOUREA
165
Acetic/orcein staining of chromosome
squashes
ACETIC ACID
164
Immunodiffusion
AGAROSE, ETHANOL, COOMASSIE BRILLIANT BLUE, ACETIC ACID
163
Calmodulin extraction
TAPEWORM, DRY-ICE, LOW TEMPERATURE, ETHANOL
162
Use of 'Prestige' domestic pressure
cookers
HIGH TEMPERATURE, HIGH PRESSURE
161
Culture of erythropoietic cell line K562
HUMAN TISSUE, LN, LIQUID NITROGEN
160
Cleaning of glassware with 'E-toxa clean'
E-TOXA CLEAN
159
Autoclaving
HIGH TEMPERATURE, HIGH PRESSURE
158
Using the Sorvall Omni ultracentrifuge
HIGH SPEED CENTRIFUGATION, VACUUM
157
Using the Sorvall TV-1665 vertical rotor
HIGH SPEED CENTRIFUGATION, VACUUM
156
Using the Sorvall T-1270 angle rotor
HIGH SPEED CENTRIFUGATION, VACUUM
155
Using the Sorvall AH-629 swing-out rotor HIGH SPEED CENTRIFUGATION, VACUUM
154
Using the Sorvall T-865 angle rotor
153
Using the Sorvall TH-641 swing-out rotor HIGH SPEED CENTRIFUGATION, VACUUM
HIGH SPEED CENTRIFUGATION, VACUUM
No.
Title
Keywords
152
Scintillation vial crusher
H3, C14, OPTIPHASE SAFE
151
Recombinant DNA practical
E. COLI, AGAROSE GEL ELECTROPHORESIS, SDS, 2-PROPANOL, ETHANOL, NaOH, MERCAPTOETHANOL,
DIMETHYLFORMAMIDE, DMF, CHLOROFORM, PHENOL, 8-HYDROXYQUINOLINE, ETHIDIUM BROMIDE, DMSO,
DIMETHYL SULPHOXIDE, UV
150
X-ray studies of Staphylococcal
enterotoxins
STAPHYLOCOCCUS AUREUS ENTEROTOXINS
149
Cell adhesion
TRYPAN BLUE, EMETINE HCl, TRYPSIN, ETHANOL, NaOH, EMETINE, CYCLOHEXIMIDE, MONENSIN, HYPODERMIC
NEEDLES, PASTEUR PIPETTES
148
Fungal mutagenesis with EMS
EMS, ETHYL METHANE SULPHONATE, MUTAGENESIS
147
Use of Sorvall RC5B and RC5C medium
speed centrifuges
CENTRIFUGES
146
Silylation of sugars
TMSI, N-TRIMETHYLSILYLIMIDAZOLE
145
The precipitation of protein onto paper
TRICHLOROACETIC ACID, ETHANOL, DIETHYL ETHER
144
Solvent use in coupling experiment
FORMAMIDE, TEREPHTHALALDEHYDE
143
Preparation of Cibacron Blue dextran
conjugates
ETHANOL, CIBACRON BLUE, SODIUM AZIDE
142
Synthesis of NAD-dextran conjugates
IODOACETIC ACID, Na BOROHYDRIDE, Na CYANOBOROHYDRIDE, 1,6-DIAMINOHEXANE, DIAMINOPROPANE, 1ETHYL-3(3-DIMETHYL-AMINOPROPYL) CARBODIIMIDE, Na PERIODATE, Na DITHIONITE, Na HYDROXIDE,
HYDROCHLORIC ACID, ACETONE
141
Cytochrome oxidase - undergraduate
practical
POTASSIUM CYANIDE
140
The culturing of Plasmodium falciparum in PLASMODIUM FALCIPARUM, MALARIA PARASITE, HUMAN SERUM, HUMAN RED BLOOD CELLS, HYPOXANTHINE
human red blood
139
Producing slides of Plasmodium
falciparum
PLASMODIUM FALCIPARUM, MALARIA PARASITE, HUMAN ERYTHROCYTES
138
Use of the X-ray detector in 6 West
BERYLLIUM, IONISING RADIATION, X-RAYS
133
Tissue culture
RATS, MICE, SYRINGE, URIDINE, DEOXYURIDINE, THIOMERSAL, CYTOSINE ARABINOSIDE, ETHANOL,
CYCLOHEXIDINE GLUCONATE, SODIUYM AZIDE
132
Cyanogen bromide activation of agarose
for folate affinity chromatography
AGAROSE GEL, ACETONITRILE, CYANOGEN BROMIDE, UREA
131
Biorad protein estimation
PHOSPHORIC ACID, COOMASSIE DYE, METHANOL
130
Protein crystallisation matrix
SODIUM CACODYLATE, ACETIC ACID, TRIS, HYDROCHLORIC ACID, PEG 4000, POLYETHYLENE GLYCOL
129
Protein assay (Lowry method)
FOLINS PHENOL REAGENT
128
Enzyme linked immumosorbent assay
(ELISA)
UNSCREENED HUMAN SERUM, SYRINGE, 3,3',5,5'-TETRAMETHYL BENZIDINE, DMSO, DIMETHYL SULPHOXIDE,
SULPHURIC ACID, SODIUM HYPOCHLORITE
127
Column chromatography - enzyme
purification
POTASSIUM HYDROXIDE, SODIUM PYROPHOSPHATE, TRIS, ACETIC ACID, ALLOPURINOL, BLUE HERD TEXTILE DYE
GEL
No.
Title
126
(Human) xanthine oxidase enzyme assay POTASSIUM FERRICYANIDE, TETRASODIUM PYROPHOSPHATE
Keywords
125
Western blotting
124
Polyacrylamide gel electrophoresis (PAGE) HIGH VOLTAGE, SYRINGE, ACRYLAMIDE, N,N-METHYLENE BISACRYLAMIDE, TEMED, AMMONIUM PERSULPHATE, 2MERCAPTOETHANOL, SDS, ACETIC ACID, METHANOL, COOMASSIE BRILLIANT BLUE, BROMOPHENOL BLUE
123
(Human) xanthine oxidase production
UNSCREENED HUMAN MILK
122
Radioassay for enzyme samples
[8-C14]-HYPOXANTHINE, METHANOL, AMMONIA, PERCHLORIC ACID, BUTANOL
121
Assessment of acetaldehyde/glycerol
production
THIOSEMICARBAZIDE, 5,5-DIMETHYL-1,3,CYCLOHEXANEDIONE, DIMEDONE
120
Transcription
P32, PHENOL, DMSO, DIMETHYL SULPHOXIDE, SYRINGE, IODOACETIC ACID, ETHANOL
119
SDS/PAGE gel electrophoresis
ACRYLAMIDE, IONISING RADIATION
118
HPLC separation of plant hormones
HPLC, NITROGEN & HELIUM CYLINDERS, ACETONITRILE, METHANOL, ACETIC ACID, SYRINGE, HIGH PRESSURES
117
Pectin analysis
ACETONE, SULPHURIC ACID, PHENOL, DMSO, DIMETHYL SULPHOXIDE, ACETIC ACID, SODIUM HYDROXIDE, 3PHENYLPHENOL
116
Fractionation of plant waxes
CHLOROFORM, PETROLEUM SPIRIT, BENZENE, ACETIC ACID, DIETHYL ETHER, POTASSIUM DICHROMATE,
SULPHURIC ACID, RHODAMINE 6G, UV
115
Cadmium salts
CADMIUM CHLORIDE
114
Purification of RNA from cultured cells
LIQUID NITROGEN, LN, GUANIDINIUM THIOCYANATE, PHENOL, CHLOROFORM, ISOPROPYL ALCOHOL,
MERCAPTOETHANOL
113
In vitro synthesis of mRNA
GENETIC MODIFICATION, ETHANOL
112
Basic glass blowing/repairing
OXYGEN GAS CYLINDER, EXTREME HEAT, NATURAL GAS, SHARPS
111
Use of Braun homogeniser
NOISE, CO2, GAS CYLINDER
110
Lipid extraction and GC analysis
DIETHYL ETHER, GAS CYLINDERS, HYDROGEN, PETROLEUM ETHER, PETROPEUM SPIRIT, BORON
TRIFLUORIDE/METHANOL
109
Beta-glucuronidase assay
GLACIAL ACETIC ACID
108
Histology - Tissue fixing and processing
BOUIN'S FIXATIVE, FORMALDEHYDE, FORMALIN, PICRIC ACID, XYLENE, ETHANOL, SHARPS, MICROTOME KNIFE,
RAZOR BLADE
107
Culture & handling of insects ; Manduca
sexta
INSECTS, DUSTS, MANDUCA WING SCALES, BLOOD
106
Colorimetric assay for DNA
DIPHENYLAMINE, ACETALDEHYDE, PERCHLORIC ACID, POTASSIUM HYDROXIDE, KOH
105
Acid phosphatase assay
P-NITROPHENYL PHOSPHATE, P-NITROPHENOL, NaOH, SODIUM HYDROXIDE
104
Protein assay (Lowry)
FOLIN-CIOCALTEU PHENOL REAGENT, SODIUM HYDROXIDE, NaOH, COPPER(II) SULPHATE
103
Histochemical staining of lysosomes with
acridine orange
ACRIDINE ORANGE, SHARPS
102
Conversion of amines to azides
SODIUM NITRITE, SODIUM AZIDE, SODIUM HYDROXIDE, NaOH, ETHYL ACETATE
101
Radioiodination of organic compounds
CHLORAMINE T, Na125I, SODIUM 125IODIDE, IONISING RADIATION
HIGH VOLTAGE, METHANOL, H2O2, HYDROGEN PEROXIDE,4-CHLORO-1-NAPHTHOL
No.
Title
Keywords
100
DNA and RNA isolation
CHLOROFORM, ISO-AMYL ALCOHOL, HCl, ETHANOL, LN, SDS, PHENOL, 2-PROPANOL, DIETHYL PYROCARBONATE,
DEP, DEPC, HYDROGEN PEROXIDE, H2O2, FORMAMIDE, 2-MERCAPTOETHANOL, SARCOSYL, GUANIDINIUM
ISOTHIOCYANATE
99
Quantitative assay of DNA
PERCHLORIC ACID, DIPHENYLAMINE, GLACIAL ACETIC ACID, ACETALDEHYDE
98
Isolation and culture of Trypanosoma
brucei
TRYPANOSOMES, TRYPANOSOMA BRUCEI, SHARPS, RATS, BLOOD
97
Phenol/chloroform extraction & ethanol
precipitation of DNA
PHENOL, CHLOROFORM, ETHANOL
96
Blotting and Hybridisation
UV, -80'C
95
Ethylene determination
ETHYLENE, HYDROGEN GAS CYLINDERS
94
Preparation of plant powders
DUST
93
Sugar determination
AMMONIUM MOLYBDATE, DISODIUM HYDROGEN ARSENATE
92
Coupling of carboxyl & amino groups
CARBODIIMIDE, N-HYDROXYSUCCINIMIDE, TRIETHANOLAMINE
91
Gel filtration
SODIUM AZIDE
90
Recycling assay for dehydrogenase
activities
PHENAZINE ETHOSULPHATE, PES, DCPIP
89
Synthesis of NAD analogs from NAD
IODOACETIC ACID, LITHIUM HYDROXIDE, HCl + POTASSIUM PERMANGANATE, SODIUM DITHIONITE, LITHIUM
CHLORIDE, CARBODIIMIDE, DIAMINOHEXANE
88
Preparation of stock solutions for
polyacrylamide electrophoresis
ACRYLAMIDE, METHYLENE BIS ACRYLAMIDE, TRIS, SDS, SODIUM DODECYL SULPHATE, AMMONIUM PERSULPHATE,
GLYCINE, HCl
87
Silanizing glassware
DICHLORO DIMETHYL SILANE, 1,1,1-TRICHLOROETHANE, ETHANOL, SILANE A-174
86
Preparation of loading dyes
XYLENE CYANOL, BROMOCRESOL GREEN, BROMOPHENOL BLUE, 2-MERCAPTOETHANOL
85
Agarose gel electrophoresis (but not
denaturing gels)
ETHIDIUM BROMIDE, MICROWAVE OVEN, HIGH VOLTAGE
84
Preparation of TRIS concentrates and
TRIS based buffers
TRIS (HYDROXYMETHYL) MRTHYLAMINE, ACETIC ACID, HCl, EDTA, ETHYLENE DIAMINE TETRAACETIC ACID,
SODIUM HYDROXIDE, BORIC ACID
83
Application of fertilisers
DUST
82
Application of pesticides
NICOTINE, TEMIK 10G, ALDICARB, GRAMOXONE 100, PARAQUAT, METASYSTOX R, OXYDEMETON, PARABLE,
GAMMA-COL, GAMMA-HCH
81
Use of horticultural machinery
TRACTOR, ROTAVATOR, BRUSHWOOD CUTTER, HEDGE TRIMMER, NOISE
80
Insect receptor studies
LOCUST, SCHISTOCIRCA GREGARIA
79
Plant specimen preservation
FORMALDEHYDE, FORMALIN, ACETIC ACID, ETHANOL
78
Class II hood fumigation
FORMALDEHYDE, FORMALIN
77
'DNA' extraction
PHENOL, 8-HYDROXYQUINOLINE, CETYLTRIMETHYL AMMONIUM BROMIDE, CETRIMIDE
76
Acids and solvents used in washing-up
areas
ISO-PROPANOL, PROPAN-2-OL, HCl, ETHANOL, ACETONE
No.
Title
Keywords
75
Formaldehyde gel
FORMALDEHYDE, FORMALIN, ETHIDIUM BROMIDE, BROMOPHENOL BLUE, XYLENE CYANOL FF
74
Phosphodiesterase assay
SNAKE VENOM FROM AUSTRALIAN KING COBRA (OPHIOPHAGUS HANNAH)
73
Preparation of & use of aphid fixative
KOH. POT. HYDROXIDE, ETHANOL, CHLOROPHENOL, CHLORAL HYDRATE
72
Use of 'Fluon' (PTFE)
PTFE SOLVENT
71
Computer VDU use
NON-IONISING RADIATION, REPEATED STRESS
70
Use of 'Pactra' paint
PAINT SOLVENTS
69
Use of organic solvents for cleaning ant
mazes and paint
ETHANOL, ACETONE
68
Cell culture
TOLBUTAMIDE, DEXAMETHASONE, ISOMETHYLBUTYLXANTHINE, LIQUID NITROGEN
67
Gel processing
SHARPS, METHANOL, ACETIC ACID, H2O2, HYDROGEN PEROXIDE, OPTIPHASE SAFE, AMMONIA
66
Photolabelling
UV, CENTRIFUGE
65
Antibody preparation
FREUNDS ADJUVANT, MBS, DMF, ETHANOLAMINE, SHARPS
64
ELISA. Enzyme linked immunosorbent
assay
H2SO4, SULPHURIC ACID, DMSO, DIMETHYL SULPHOXIDE, TETRAMETHYLBENZIDINE, H2O2, HYDROGEN PEROXIDE
63
Immunoprecipitation
CENTRIFUGE, SHARPS, LEUPEPTIN, OTHER PROTEASE INHIBITORS
62
Transport assays
PHLORETIN, NaOH, SODIUM HYDROXIDE, SCINTILLANT, OPTIPHASE
61
Protein assays
NaOH, SODIUM HYDROXIDE, BIO-RAD REAGENT, LOWRY, BCA
60
Western blotting
METHANOL, TCA, TRICHLORACETIC ACID, SDS, 125I, NP40, DIETHANOLAMINE, NITROBLUE TETRAZOLIUM,
BROMOCHLORO INDOLYL PHOSPHATE
59
Preparation of cells and membranes from PMSF, PHENYLMETHYL SULPHONYL FLUORIDE, LEUPEPTIN, HUMAN BLOOD, RATS, CENTRIFUGE
rats & blood & 3T3-Li cells
58
SDS PAGE polyacrylamide gel
electrophoresis
ACRYLAMIDE, BIS-ACRYLAMIDE, SDS, SODIUM DODECYL SULPHATE, TEMED, MERCAPTOETHANOL, AMMONIUM
PERSULPHATE, UREA, NN'METHYLENE BIS ACRYLAMIDE, HV ELECTRICITY
57
Treatment of prepared cells
KCN, POT. CYANIDE, OKADEIC ACID, DIGITONIN, TOLBUTAMIDE, CYCLOHEXIMIDE, DEXAMETHASONE,
STREPTOZOTOCIN, MBS, 5-MBS, 5-SMPB, PAO, PHENYL ARSENOXIDE, FDNB, DINITROFLUOROBENZENE
56
Performing API 20E tests
ACDP HAZARD CATEGORY 2, INDOLE, ISO-AMYL ALCOHOL, PARADIMETHYLAMINOBENZALDEHYDE, HCl ACID,
FERRIC CHLORIDE, ALPHA-NAPHTHOL, POT HYDROXIDE, SULPHANILIC ACID, ACETIC ACID, N,N-DIMETHYL-ALPHANAPHTHYLAMINE, TETRAMETHYL-P-PHENYLENE DIAMINE
55
Preparation and use of a vital stain trypan blue
TRYPAN BLUE, ACDP HAZARD CATEGORY 2 BACTERIA
54
Work with ACDP hazard category 2
bacteria
ACDP HAZARD CATEGORY 2 BACTERIA, SALMONELLA SPP., PSEUDOMONAS PUTIDA/FLUORESCENS, MICROCOCCUS
SPP., ESCHERICHIA COLI, STAPHYLOCOCCUS AUREUS, AEROMONAS SPP
53
Determination of total free amino acids
SODIUM CYANIDE, SODIUM ACETATE, ACETIC ACID, NINHYDRIN, METHYL CELLOSOLVE, ETHYLENE GLYCOL
MONOMETHYL ETHER, ISO-PROPYL ALCOHOL, PROPAN-2-OL
52
Ammonia determination
PHENOL, SODIUM NITROPRUSSIDE, SODIUM NITROSOPENTACYANOFERRATE(III), SODIUM HYDROXIDE, SODIUM
HYPOCHLORITE
51
Total sugar determination
TCA, TRICHLORACETIC ACID, SULPHURIC ACID, THIOUREA, ANTHRONE
No.
Title
Keywords
50
Fumigation of Class II biological safety
cabinet
FORMALIN, FORMALDEHYDE
49
Producing diazo 'blue' slides
UV SOURCE, AMMONIA SOLUTION
48
Iodination of proteins
SODIUM 125IODIDE, Na125I, CHLORAMINE T, XYLENE CYANOL, SODIUM HYDROXIDE
47
Hepatitis A virus radioimmunofocus assay HEPATITIS A VIRUS, HAV, FORMALIN, FORMALDEHYDE, 125I
46
Hepatitis A virus plaque assay
HEPATITIS A VIRUS, HAV, FORMALIN, FORMALDEHYDE
45
Photographic developing and printing
PHOTO-FLO, ACETIC ACID STOP BATH, D76 DEVELOPER POWDER, UNIFIX LIQUID FIXER, T-MAX DEVELOPER, TMAX POSITIVE KIT, KODALITH DEVELOPER, DEKTOL POWDER
44
Preparation of plasmid DNA
SODIUM HYDROXIDE, SDS, CAESIUM CHLORIDE, ETHIDIUM BROMIDE, PHENOL/CHLOROFORM, AMYL ALCOHOL,
ULTRACENTRIFUGATION, UV, ETHANOL, PROPANOL, HYPODERMIC NEEDLE
43
Use of butanol derivatives in antimicrobial 3-METHYL-1-BUTANOL, 3-METHYL-1-BUTANAL, 3-METHYL-1-BUTANOIC ACID
studies
42
Use of radioisotopes
UNSEALED SOURCES, IONISING RADIATION
41
Liquid scintillation counting
SCINTILLANT, OPTIPHASE, IONISING RADIATION
40
Cell irradiation
IONISING RADIATION
39
Use of mitogens in cell culturing
CONCANAVALIN A (LECTIN FROM CANAVALIA ENSIFORMIS)
38
Extraction and identification of fungal
metabolites and enzymes
POT CYANIDE, ALPHA-NAPHTHOL, P-CRESOL, TEEPOL, L-DOPA, AZOCOLL, F&C'S PHENOL REAGENT,
SYRINGALDAZINE, ACETIC ACID, ETHYL ACETATE, BIORAD DYE REAGENT, PROTEASE
37
Use of band saw
DUSTS (INCLUDING HARDWOOD AND FUNGAL SPORES), NOISE, SHARP HIGH-SPEED BLADE
36
Use of metabolic inhibitors in experiments CETYL TRIMETHYL AMMONIUM BROMIDE (CETRIMIDE), 2.4-DINITROPHENOL, POTASSIUM CYANIDE, ACTINOMYCIN
on fungal development
D, SODIUM ARSENATE, 4-HYDROXYCOUMARIN, ANTIMYCIN, 5-AZACYTIDINE, SALICYL HYDROXAMIC ACID,
ROTENONE
35
DNA preparation and extraction
CHLOROFORM, SODIUM HYDROXIDE, SDS, PHENOL, ETHIDIUM BROMIDE, ISO-AMYL ALCOHOL, ISO-PROPANOL,
ETHANOL, ACETIC ACID
34
Microwaving
MICROWAVE RADIATION
33
Agarose gel electrophoresis
HV ELECTRICITY, ETHIDIUM BROMIDE
32
Preparation of bacteriological media
DUST
31
Silanizing glassware
DIMETHYL DICHLOROSILANE, 1,1,1-TRICHLOROETHANE
30
Western Blotting
HIV+ SERUM, HV ELECTRICITY, SODIUM AZIDE, NITRO BLUE TETRAZOLIUM, 4-CHLORO-1-NAPHTHOL, HYROGEN
PEROXIDE, SODIUM HYPOCHLORITE (CHLOROS)
29
Polymerase chain reaction amplification of
DNA
28
Autoclaving
HIGH TEMPERATURE, HIGH PRESSURE, STEAM
27
Enzyme assays
PHENYLARSENOXIDE, PMSF (PHENYL METHYL SULPHONYL FLUORIDE), DIGITONIN
26
Column chromatography
DIETHANOLAMINE, ETHANOL, TRIS
No.
Title
Keywords
25
Gel electrophoresis
AGAROSE, DIETHYL PYROCARBAMATE, COOMASSIE (BLUE R-250/BRILLIANT BLUE G-250), SDS, HCl ACID, 2MERCAPTOETHANOL, TEMED, AMM PERSULPHATE, MOPS, FORMAMIDE, FORMALDEHYDE, UREA, ETHIDIUM
BROMIDE, BISACRYLAMIDE, ACRYLAMIDE, HV ELECTRICITY
24
Cell culture
TRYPAN BLUE, HYDROCHLORIC ACID, SODIUM HYDROXIDE, TRYPSIN, ETHANOL, PPS, MONENSIN, EMETINE,
CYCLOHEXIMIDE, UV
23
Maintaining Hymenolepis diminuta
TENEBRIO MOLITOR BEETLES, TAPEWORMS, CYSTICERCOID, RATS, LIQUID NITROGEN, LN, BRAN DUST, SAWDUST
22
DNA analysis
DIPHENYLAMINE, ACETIC ACID, SULPHURIC ACID, SODIUM HYDROXIDE, SODIUM PHOSPHATE, ETHANOL,
ACETALDEHYDE, DIETHYL ETHER, PERCHLORIC ACID, EDTA, BIS BENZAMIDE
21
Changing ink cartridge in Epson inkjet
printers
POTASSIUM HYDROXIDE, KOH
20
Animal enzyme assays
COBALT CHLORIDE, HYDROCHLORIC ACID, HCl
19
S35 incorporation in proteins
S35-CYSTEINE, UREA, MERCAPTOETHANOL, IODOACETIC ACID, TRICHLOROACETIC ACID
18
RNA extraction
LIQUID NITROGEN, LN, UREA, LITHIUM CHLORIDE, CAESIUM CHLORIDE, PHENOL:CHLOROFORM, ETHANOL, 2MERCAPTOETHANOL
17
Nitration of peptides
TETRANITROMETHANE, TNM
16
Radioimmunoassays
I125-LABELLED PEPTIDES, H3-LABELLED STEROIDS, ACTIVATED CHARCOAL, SODIUM AZIDE, THIOMERSAL
15
Expression of genes using the Baculovirus BACULOVIRUS, HIGH SPEED CENTRIFUGATION
system
14
Preparation of ethidium bromide solutions ETHIDIUM BROMIDE, DEPC
13
Diethyl pyrocarbamate treatment of
glassware
DIETHYL PYROCARBAMATE (DEPC)
12
End labelling DNA - Random prime
labelling of DNA using [32P]
ALPHA [32P]DATP
11
RNA polymerase assay
TRICHLORACETIC ACID, TCA, ETHANOL, RIFAMPICIN
10
Hepatitis A virus/cell binding assay
H3/I125 LABELLED HEPATITIS A VIRUS, FORMALIN, FORMALDEHYDE, SODIUM HYDROXIDE
9
ELISA for Hepatitis A virus
HEPATITIS A VIRUS, HAV, P-NITROPHENYL PHOSPHATE
8
Oligonucleotide synthesis
ACETONITRILE, TETRAZOLE, N-METHYLIMIDAZOLE, TETRAHYDROFURAN, LUTIDINE, ACETIC ANHYDRIDE, IODINE,
PYRIDINE
7
Purification of synthetic oligonucleotides
AMMONIA, ETHANOL
6
Cryopreservation of microbial cultures
using liquid nitrogen
LIQUID NITROGEN
5
Decontamination by autoclaving
AUTOCLAVING, CATEGORY 2 PATHOGENS
4
Use of bleach for microbiological
decontamination
BLEACH, SODIUM HYPOCHLORITE, CHLORINE
3
Use of chain saw
CHAIN SAW, PETROL, FAST-MOVING SHARP BLADE
2
Use of chemicals as growth substrates for D-AMYGDALIN, P-HYDROXYBENZOIC ACID, D-GLUCURONIC ACID, MALONIC ACID, MUCIC ACID
bacteria
No.
Title
Keywords
1
Culture and purification of Hepatitis A
virus
HEPATITIS A VIRUS, HAV, SODIUM DODECYL SULPHATE, SDS, SODIUM LAURYL SULPHATE