Download Columbia Stem Cell Day 2014 - Columbia Stem Cell Initiative

Columbia Stem Cell Day 2014
April 1, 2014
Myrna Daniels Auditorium and the Riverview Terrace; Vivian and Seymour
Family Heart Center; 173 Fort Washington Avenue, New York, NY 10032
Photo: Míriam Navarro Sobrino
Photo: Masa Yazawa
Program
Program inquiries: Hynek Wichterle [email protected]
General inquiries: Dola Sengupta [email protected]
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
2
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
Columbia Stem Cell Day
April 1, 2014
9:30 AM
Breakfast
10:00 AM Welcome
10:05 AM Asa Abeliovich
10:30 AM John Dittmar (Rothstein lab)
10:45 AM Laura Johnston
11:10 AM Coffee break+ poster session
11:55 AM Stephen Tsang
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12:20 PM
LUNCH/Poster session
1:40 PM
Overview of the Stem Cell Core Facility
1:55 PM
Cathy Mendelsohn
2:20 PM
Larry Luchsinger (Snoeck lab)
2:35 PM
Coffee break+ poster session
3:25 PM
Joji Fujisaki
3:50 PM
Lee Goldman
4:00 PM
keynote lecture Peggy Goodell
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
Short talks
CLIK: A method using gene interaction density
to assess genome-wide screen results reveals
the importance of ribosome function in the
decision to differentiate into a stem cell.
John Dittmar, Robert J.D. Reid, and
Rodney Rothstein
Columbia University Medical Center, Dept. Genetics & Development,
701 West 168th Street, New York, NY 10032
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The Role of Prdm16 and Mitochondrial
Dynamics in Hematopoietic Stem Cell Function
Larry Luchsinger, Kenjiro Kamezaki, David Corrigan, &
Hans-Willem Snoeck
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
Posters
Heart Center Riverview Terrace
Electrophysiology and Ca2+ Imaging in the Helmsley
Stem Cell Core Facility at Columbia University
Cholinergic Signaling Promotes the Activation of
Adult Quiescent Neural Stem Cells
Damian Williams, Kevin Sampson, Robert Kass, Amy MacDermott
Alex Paul and Fiona Doetsch
Columbia University Stem Cell InitiativeDepartments of Pathology and Cell
Biology, Pharmacology, Physiology, and Neuroscience
Helmsley Stem Cell Core Facility: Cell Production
Facilities for Motor Neurons and Cardiomyocytes:
Dosh Whye 1,2, Ray Funahashi 1,2, Dario Sirabella 1,2,
Christopher E. Henderson 1,2, Hynek Wichterle 1,2, Gordana
Vunjak-Novakovic and Barbara Corneo 1,2
High Efficient Reprogramming of Patient Skin
Fibroblasts for Disease Modeling
LouJin Song1,2,3, Elizabeth Han1,2,4 Daniel W. Awari1,2 and
Masayuki Yazawa1,2,3
1Columbia University Stem Cell Initiative, CUMC; 2Department
Rehabilitation and Regenerative Medicine, CUMC; 3Department
Pharmacology, CUMC; 4Barnard College
of
of
Department of Pathology and Cell Biology 2 Center for Motor Neuron Biology
and disease
Columbia Genome Center
Screening Core Facility
High
Throughput
Andrea Califano, Christopher E. Henderson, Charles Karan, Hai Li,
Sergey Pampou, Ronald Realubit, Jan Michael Austria
Generation of induced pluripotent stem cells from
revertant mosaic keratinocytes
N Umegaki-Arao 1, A M.G. Pasmooij 3, M Itoh 1, J E. Cerise 1, Z Guo 1,
A Gostyński 3, M F. Jonkman 3, A M. Christiano 1, 2
Dept of Dermatology 1 and Genetics and Development 2, Columbia University,
New York; Dept of Dermatology, University of Groningen 3
The Organic Chemistry Collaborative Center (OCCC)
Black Building 1029, CUMC Campus
Generation of induced pluripotent stem cells
from conjunctiva
Shi-Xian Deng 1,2,3, Donald Landry1,2,3, Alison Rinderspacher1,2,3
Jin Yang Yao Li Deniz Erol Wen-Hsuan Wu Yi-Ting Tsai XiaoRong Li Richard J. Davis Stephen H. Tsang
1Department
of Medicine; 2Columbia University Medical Center; 3Columbia
Stem Cell Initiative
Statins Promote Axonal Regeneration by Inhibition
of Protein Prenylation
Hai Li1,5, Takaaki Kuwajima1, Elena Nikulina3, Jianwei Hou3, Wan
Seok Yang1,2, Timothy Spencer1, Gist Croft1,2, Dosh W. Whye1,2,
Emily R. Lowry1,2, Nuno J. Lamas1,2, Hynek Wichterle1,2, Marie T.
Filbin3,*, Brent Stockwell1,4, Christopher E. Henderson1,2
Stem Cell Initiative, Center for Motor Neuron Biology and Disease,
Departments of Rehabilitation and Regenerative Medicine, Pathology and Cell
Biology, Neurology and Neuroscience2Project A.L.S./Jenifer Estess Laboratory
for Stem Cell Research3Biology Department, Hunter College, City University of
New York4Howard Hughes Medical Institute, Department of Biological Sciences
and Department of Chemistry, Columbia University5NYSTEM High-Throughput
Screening and Chemistry Shared Facility
Bernard and Shirlee Brown Glaucoma Laboratory, Edward S. Harkness Eye
Institute, Columbia University Medical Center, 160 Fort Washington Ave
Research Annex, Room 513, New York, NY 10032, USA
Mitochondrial Dynamics in Human Embryonic Stem
Cells
Melanie Mumau, Larry Luchsinger, Hans Snoeck
1Columbia
*this work is dedicated to the memory of Marie Filbin, deceased
January 15th, 2014
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Epigenetic Modifiers of iPSC Reprogramming
Claudia A. Doege1, Keiichi Inoue1, Skylar Travis1, David B. Rhee1,
Ryousuke Fujita1, Paolo Guarnieri2, Alan Shih3, Ross L. Levine3,
Emily I. Chen4, Asa Abeliovich1
1 Columbia
University, Depts. of Pathology, Cell Biology, Neurology, New York,
NY
2 Columbia University, Biomedical Informatics Shared Resources, New York, NY
3 Human Oncology & Pathogenesis Program, MSKCC, New York, NY
4 Stony Brook University Proteomics Center, Stony Brook, NY
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
The Role of PRDM16 in Leukemic Stem Cell
Maintenance
Human iPS Cell Derived Cardiac Microtissue
Platform for Predictive Toxicity Studies
David Corrigan
Kacey Ronaldson, Stephen Ma, Timothy Chen, Keith Yeager, Dario
Sirabella, and Gordana Vunjak-Novakovic
Hans Snoeck Lab
Laboratory for Stem Cells and Tissue Engineering
Department of Biomedical Engineering, Columbia University
Conditioning of stem cells by electrical, mechanical
and immunological stimuli
Aida Llucia-Valldeperas1, Donald O. Freytes2, Carolina SolerBotija1, Benjamin Sanchez3, Ramon Bragos4, Antoni Bayes‐
Genis1, Gordana Vunjak-Novakovic5.
1 ICREC Research Program, Health Science Research Institute Germans Trias i
Pujol, Badalona, Spain. 2 The New York Stem Cell Foundation Research
Institute, New York, USA. 3 Department of Neurology, Division of
Neuromuscular Diseases, Beth Israel Medical Deaconess Center, Harvard
Medical School, Boston, USA. 4 Electronic and Biomedical Instrumentation
Group, Departament d’Enginyeria Electrònica, Universitat Politècnica de
Catalunya, Barcelona, Spain. 5 Laboratory for Stem Cells and Tissue
Engineering, Department of Biomedical Engineering, Columbia University, New
York, USA.
Asymmetric Inheritance of Pax5 May Balance Selfrenewal and Terminal Differentiation of B
Lymphocytes
Wen-Hsuan W. Lin and Steven L. Reiner
Departments of Microbiology & immunology and Pediatrics, College of
Physicians & Surgeons, Columbia University, New York, NY 10032
Directed hPSC differentiation to thymic epithelial
cells.
Rafael Gras, Hans W. Snoeck
The Hippo-Yap Pathway Controls Differentiation of
Airway Epithelial Progenitors in the Developing and
Adult Lung.
John E. Mahoney1,2,, Munemasa Mori1, Aleksander D. Szymaniak3,
Xaralabos Varelas3, Wellington V. Cardoso1,2
1 Columbia Center for Human Development, Department of Medicine, Columbia
University Medical Center. New York, NY 10032; 2 Pulmonary Center, Boston
University School of Medicine, Boston, MA 02118 ; 3.Department of
Biochemistry, Boston University School of Medicine, Boston, MA 02118.
Generation
derivatives.
of
anterior
foregut
endoderm
Ya-Wen Chen, Jingxin Xia, Sarah Xuelian Huang, Megan Skyes,
Hans-Willem Snoeck
Using Induced Pluripotent Stem Cells to Investigate
the Central Nervous System Phenotypes of PraderWilli Syndrome
Lisa C. Burnett1,2, Carlos R. Sulsona3, Daniel J. Driscoll3, Dieter
Egli1,4, Rudolph L. Leibel1,2,5
1 Columbia University, 2 Institute of Human Nutrition, 3 University of Florida, 4
New York Stem Cell Foundation, 5 Naomi Berrie Diabetes Center. Supported by
FPWR Research Grant, FPWR/PWSA PWS Best Idea Grant.
Exogenous COL18A1 Restores Retinal Function in a
Patient Specific Model of Knobloch Syndrome
Huy V. Nguyen, Yao Li, Susanne Koch, Jin Yang, Irene Maumenee,
Stephen H. Tsang
Electrical Conditioning of Human Embryonic Stem
Cell Derived Cardiomyocytes
George Eng1,2, Benjamin W. Lee1,2, Lev Protas3, Mark Gagliardi4,
Kristy Brown5, Gordon Keller4, Richard B. Robinson3, and
Gordana Vunjak-Novakovic1,6
1Department
of Biomedical Engineering, Columbia University, New York, NY
10027 2College of Physicians and Surgeons, Columbia University, New York, NY
10032 3Department of Pharmacology, Columbia University, New York, NY
10032 4McEwen Centre for Regenerative Medicine, University Health Network,
Toronto, Ontario M5G1L7, Canada 5Department of Pathology and Cell Biology,
Columbia University, New York, NY 10032 6Department of Medicine, Columbia
University, New York, NY 10032
Timing regulation for efficient differentiation of
human induced pluripotent stem cells into
cardiomyocytes
Dario Sirabella, William Tang & Gordana Vunjak-Novakovic
Columbia University Medical Center, Department of Biomedical Engineering
and Department of Rehabilitation and Regenerative Medicine, Vanderbilt Clinic
12-234, New York, NY 10032
Generation of pigmented human 3D Skin
equivalents containing iPSC-derived melanocytes,
keratinocytes and fibroblasts
Karl Gledhill, Zongyou Guo, Munenari Itoh, Claire A. Higgins,
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Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
Noriko Umegaki, Angela M. Chrstiano
networks identifies a synergistic interaction
between FOXM1 and CENPF that drives prostate
cancer malignancy
Site-specific genome editing using TALENs for
correction of dominant dystrophic epidermolysis
bullosa
Antonina Mitrofanova2,*,Alvaro Aytes1,8*, , Celine Lefebvre2,*,^,
Mariano J. Alvarez2, Mireia Castillo-Martin9, Tian Zheng7,10, James
A. Eastham11, Anuradha Gopalan12, Kenneth J. Pienta13, Michael
M. Shen2,3,4,7, Andrea Califano2,5,7, and Cory Abate-Shen1,2,6,7
Satoru Shinkuma1, Angela M Christiano1,2
Departments of Dermatology1 and Genetics and Development2, Columbia
University, New York, U.S.A
Notch3 signaling identifies a subpopulation of p63negative airway progenitors with features of
suprabasal cells in the lung.
Munemasa Mori, John E. Mahoney, Hong Zhang, Jining Lu, Jesus
Paez-Cortez, James Schwob, Wellington. V. Cardoso
Columbia Center for Human Development, Department of Medicine, Columbia
University Medical Center. New York, NY 10032; Pulmonary Center, Boston
University School of Medicine, Boston, MA 02118 ; University of Massachusetts
Medical School, MA; Department of Molecular & Cell Biology, Tufts University,
Boston, MA
CXCL12-CXCR4 axis supports single Mist1+ gastric
stem cell niche and contributes to cancer
development.
Yoku Hayakawa, Hiroshi Ariyama, Samuel Asfaha, Daniel L.
Worthley, Christoph B. Westphalen, Hongshan Wang, Ashlesha
Muley, Yagnesh Tailor, Karan Nagar, Michael D. Gershon, Lei
Ding, and Timothy C. Wang
Innate immune pathways trigger elimination of
loser cells during cell competition
Cora Bergantiños, Marc Amoyel, Claire de la Cova, Laura A
Johnston
Department of Genetics and Development Columbia University, New York, NY
Expanding the Synthetic Capabilities of the Cell
Virginia Cornish’s lab
Departments of 1Urology, 2Systems Biology, 3Medicine, 4Genetics &
Development, 5Biochemistry and Molecular Biophysics, and 6Pathology and Cell
Biology; 7Herbert Irving Comprehensive Cancer Center, Columbia University
Medical Center, New York, NY 10032;
8Translational Research Laboratory, Catalan Institute of Oncology, Bellvitge
Institute for Biomedical Research, Barcelona, Spain 08907;
9Department of Pathology, Icahn School of Medicine at Mount Sinai New York,
10Department of Statistics, Columbia University, New York, NY 10027;
Departments of 11Urology and 12Pathology, Memorial Sloan Kettering Cancer
Center, New York, NY 10065;
13The University of Michigan Ann Arbor, MI 48109, and the Brady Urological
Institute at the Johns Hopkins School of Medicine, Baltimore, MD 21231;
*Equal contribution
Radioresistant Krt19+ Cells give rise to Lgr5+ Crypt
Base Columnar Cells and maintain gut homeostasis
following radiation injury in vitro.
Ashlesha Muley1, Samuel Asfaha1, Yoku Hayakawa1, Yagnesh
Tailor1, and Timothy C. Wang1
Division of Digestive and Liver Diseases, Department of Medicine, Columbia
University
Transcription factor mediated enhancer-promoter
connectivity programs motor neuron identity
Michael Closser1, Christopher Reeder2, Yijun Ruan3, David K.
Gifford2, Hynek Wichterle1
1 Departments of Pathology and Cell Biology, Neurology, and Neuroscience,
Center for Motor Neuron Biology and Disease, Columbia Stem Cell Initiative,
Columbia University Medical Center, New York, New York, USA 2 Computer
Science and Artificial Intelligence Laboratory, Massachusetts Institute of
Technology, Cambridge, Massachusetts, US 3 The Jackson Laboratory for
Genomic Medicine, and Department of Genetic and Development Biology,
University of Connecticut
Syntax of dynamic Isl1 binding sites in maturing
motor neurons revealed by ChIP-exo
Ho Sung Rhee and Hynek Wichterle
A role for Spz in sensing and signaling during cell
competition
Lale Alpar1, Cora Bergantiños2, Marc Amoyel2, Laura A. Johnston2
1Department of Biological Sciences, Columbia University, New York, NY
2Department of Genetics & Development, Columbia University, New York, NY
Cross-species analysis of genome-wide regulatory
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Probing diversity of stem cell derived motor
neurons with single cell expression profiling
Chris Tan and Hynek Wichterle
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
Christopher Henderson1.
Proteomics and Personalized Medicine: Protein
Profiling Identifies SOD2 as a Mediator of
Antioxidative
Defense
Associated
with
Susceptibility in Age-Related Macular Degeneration
in Patient-Specific Stem Cell Lines
Jin Yang1,2,3, Yao Li1,3, Lawrence Chan1,3, Yi-Ting Tsai1,3, WenHsuan Wu1,3, Huy V. Nguyen1, Chun-Wei Hsu, Lewis M.Brown5,
Dieter Egli4, Janet R. Sparrow3, Stephen H. Tsang1,3,6
1Bernard
& Shirlee Brown Glaucoma Laboratory, Departments of
Ophthalmology, Pathology & Cell Biology, College of Physicians and Surgeons,
Columbia University, New York, NY 10032, USA
2Tianjin Medical University Eye Hospital, TianJin 300384. China
3Edward S. Harkness Eye Institute, Columbia University, New York, NY 10032,
USA
4The New York Stem Cell Foundation Laboratory, New York, NY 10032, USA
5Quantitative Proteomics Center, Department of Biological Sciences, Columbia
University, New York, NY 10027
6New York-Presbyterian Hospital/Columbia University Medical Center, New
York, NY 10032, USA
1Project
A.L.S./Jenifer Estess Laboratory for Stem Cell Research, Departments of
Regenerative Medicine, Pathology, Neurology and Neuroscience, Center for
Motor Neuron Biology and Disease (MNC), Columbia Stem Cell Initiative (CSCI),
Columbia University Medical Center, New York, USA
2 iPierian, Inc., South San Francisco, California, USA
3The Howard Hughes Medical Institute, Harvard Stem Cell Institute,
Department of Stem Cell and Regenerative Biology, Department of Molecular
and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
ApoE influences injury-induced neurogenesis in an
isoform-specific manner
Sue Hong, MD - Pediatric Critical Care Clinical Fellow
PI: Steven Kernie
Dept of Pediatrics, Division of Pediatric Critical Care Medicine
Specification and subtype diversification of ESCderived spinal interneurons
Phuong Hoang, Hynek Wichterle
Emerging Roles of Motor Neuron Hyperexcitability
in an iPSC Model of ALS
Emily Rhodes Lowry1-4, Qinghong Yan2,5,6, Damian J. Williams2,7,8,
Derek H. Oakley1-4, Kevin Eggan9, Chaolin Zhang2,5,6, Hynek
Wichterle1-4, Christopher E. Henderson1-4.
1Project
A.L.S./Jenifer Estess Laboratory for Stem Cell Research, 2Center for
Motor Neuron Biology and Disease, 3Departments of Pathology and Cell Biology,
Neurology, and Neuroscience, 4Columbia Stem Cell Initiative, 5Department of
Systems Biology, 6Department of Biochemistry and Molecular Biophysics,
7Columbia Electrophysiology Core, 8 Department of Physiology and Cellular
Biophysics, Columbia University Medical Center, New York, NY. 9Harvard Stem
Cell Institute, Department of Stem Cell and Regenerative Biology, Harvard
University, Cambridge, MA.
Transgenic human ES and IPS cells as a tool to study
motor neuron diseases
Mathieu Desclaux1, Jackson Sandoe2, Yoon Kim1, Jimmy Hom1,
Alejandro Garcia Diaz1, Kevin Eggan2, Hynek Wichterle1 and
Christopher Henderson1.
1Project
A.L.S./Jenifer Estess Laboratory for Stem Cell Research, Departments of
Regenerative Medicine, Pathology, Neurology and Neuroscience, Center for
Motor Neuron Biology and Disease (MNC), Columbia Stem Cell Initiative (CSCI),
Columbia University Medical Center, New York, USA
2The Howard Hughes Medical Institute, Harvard Stem Cell Institute,
Department of Stem Cell and Regenerative Biology, Department of Molecular
and Cellular Biology, Harvard University, Cambridge, Massachusetts, USA
An SMN-dependent, cell-autonomous survival
deficit in human Type 1 SMA ES-derived motor
neurons
Mathieu Desclaux1, Yoon Kim1, Alejandro Garcia Diaz1, Shila
Mekhoubad3, John Dimos2,3, Kevin Eggan3, Hynek Wichterle1 and
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A stem cell model of the motor circuit reveals
distinct requirements of SMN for motor neuron
survival and function.
Christian M. Simon1,2, Anna M. Janas1,2, Francesco Lotti1,2, Livio
Pellizzoni1,2, George Z. Mentis1,2
1. Center for Motor Neuron Biology and Disease,
2. Dept. of Pathology and Cell Biology, Columbia University, NY, 10032
Investigating
amyotrophic
lateral
sclerosis
pathophysiology by functional characterization of
motor neurons generated from mouse embryonic
stem cells
John W. Smerdon1, Damian J. Williams4, Emily R. Lowry2,4,
Sebastian Thams2, Christopher E. Henderson2,3,4,5, Amy B.
MacDermott1,3 and Hynek Wichterle2,3,4,5
1Department
of Physiology and Cellular Biophysics, Columbia University, New
York, NY. 2Project A.L.S./Jenifer Estess Laboratory for Stem Cell Research, New
York, NY. 3Department of Neuroscience, Columbia University, New York, NY.
4Department of Pathology, Columbia University, New York, NY. 5 Department of
Neurology, Center for Motor Neuron Biology and Disease (MNC), and Columbia
Stem Cell Initiative (CSCI), Columbia University, New York, NY.
Evaluation of the role of the retinal G proteincoupled receptor (RGR) using patient-specific iPSderived RPE-cells
Susanne Koch1,2, Yao Li1,2, Huy V. Nguyen1,2, Yang Jin1,2, Stephen H.
Tsang1,2
1Brown
Glaucoma Laboratory, Department of Pathology and Cell Biology,
College of Physicians and Surgeons, Columbia University, New York, New York
Columbia Stem Cell Initiative
Tapping the Potential of Stem Cells for Human Health
10032, USA
2Edward S. Harkness Eye Institute, and Department of Pathology and Cell
Biology, College of Physicians and Surgeons, Columbia University, New York,
New York 10032, USA
Prospective identification and purification of
quiescent adult neural stem cells from their in vivo
niche
Paolo Codega, Violeta Silva-Vargas, Alex Paul, Angel R
Maldonado-Soto, Annina M DeLeo, Erika Pastrana and Fiona
Doetsch
Angiogenesis and neurogenesis in the ageing human
dentate gyrus
Adrienne N. Santiago1, Tanya H. Butt1, Andrew J. Dwork1,3,4,
Gorazd B. Rosoklija1,3,7, Victoria Arango1,3, René Hen2,3,5,6, J. John
Mann1,3, Maura Boldrini1,3
Divisions of 1Molecular Imaging and Neuropathology and 2Integrative
Neuroscience, New York State Psychiatric Institute; Departments of 3Psychiatry,
4Pathology and Cell Biology, 5Neuroscience and 6Pharmacology, Columbia
University, New York, NY, USA; 7Macedonian Academy of Sciences & Arts,
Republic of Macedonia
Identification of direct Nkx2.2 targets involved in
the repression of motor neuron identity
Abarinov, E. V., Mazzoni, E. O., Closser, M. C., O'Donnell, C., Guo, Y.,
Balderes, D. A., Morrison, C. A., Gifford, D. K., Wichterle, H. Sussel,
L.
MicroRNA Regulation of Adult Neural Stem Cells
Annina DeLeo, Natalia Molotkova, and Fiona Doetsch
Retinal ganglion cell genesis and
determination in the binocular circuit
subtype
Florencia Marcucci, Qing Wang, Takaaki Kuwajima, Sania Khalid,
Punita Bhansali, Ilana Rayport, and Carol Mason
Departments of Pathology & Cell Biology, Neuroscience and Ophthalmology
Gene therapy on patient-specific stem cell lines with
MFRP defect
Yao Li, Wen-Hsuan Wu, Chun-Wei Hsu, Huy V. Nguyen, Yi-Ting
Tsai, Takayuki Nagasaki, Irene H. Maumenee, Lawrence A.
Yannuzzi, Quan V. Hoang, Haiqing Hua, Dieter Egli, Stephen H.
Tsang.
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