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台中班 2012 暑期生化小考
1.下列何者不屬於 glycosaminoglycan？
(A) peptidoglycan (B) mucopolysaccharide (C) heparin (D) hyaluronic acid
2. Which one of the following description is correct？
(A) The major form of sugar is L formbody.
(B) The major form of the glucose in solution is β-D-glucopyranose.
(C) One of the structural elements of nucleic acids is D-glucose.
(D) Fructosemia (high levels of fructose in the body) occurs when fructose cannot be converted to
UDP-galactose at a normal rate in the cells.
3. Which one of the following compounds is the end product of glycolysis under anaerobic condition
in exercising muscles？ (A) 2,3-Bisphosphoglycerate (B) Lactate (C) Pyruvate (D) Acetate.
4. Phosphofructokinase, catalyzing the formation of fructose 1,6-bisphosphate from fructose
6-phosphate,
(A) readily catalyses the reverse reaction under physiological conditions
(B) is allosterically activated by ATP
(C) is allosterically inhibited by citrate
(D) is absent from skeletal muscle.
5. Glucose-Alanine Cycle 在那些器官進行？
(A) Liver-Brain (B) Liver-Muscle (C) Muscle-Blood (D) Muscle-Kidney。
6. The metabolic process by which glucose is synthesized in liver is
(A) Glycolysis (B) Gluconeogenesis (C) Glycogenesis (D) Glycogenolysis
7. Which one of the following descriptions is not correct？
(A)Glucose enters into the glycolytic pathway by phosphorylation to glucose-6-phosphate,
accomplished by the enzyme hexokinase.
(B)Pyruvate is oxidized to acetyl-CoA by an enzyme known as pyruvate kinase.
(C)Oxidation of 1 mole glucose yields up to 38 moles of ATP under aerobic condition.
(D)Pyruvate dehydrogenase is regulated by end-product (e.g., Acetyl-CoA and NADH) inhibition.
8.下列那些酵素所催化的反應有 CO2 及 NADH 的產生：
(A)lactate dehydrogenase
(B)pyruvate dehydrogenase complex
(複選題)
(C)isocitrate dehydrogenase
(D)6-phosphogluconate dehydrogenase
(E)α–ketoglutarate dehydrogenase complex.
9. For gluconeogenesis, which one of the following descriptions is not correct.
(A) Glucogenic amino acids are substrate for gluconeogenesis.
(B) Gluconeogenesis is has a nonoxidative phase, which provides ribose precursors for nucleotide
synthesis.
(C) Blood glucose can be derived from gluconeogenesis.
(D) Fructose 2,6-bisphosphate plays a unique role in the regulation of glycolysis and
gluconeogenesis in liver.
10. Please choose the correct answer from the following descriptions:
(1) Amylose is unbranched; amylopectin and glycogen contain many (α1→4) branches.
(2) Starch is the most abundant polysaccharide in nature.
(3) Dextran is homopolysaccharide.
(4) The galactose unit of the compound Gal(β1→4)Glc residue is at the reducing end.
(5) D-glucose and D-glucuronic acid is different on C6.
(A) 1, 2, and 5 are incorrect.
correct.
(B) 2 and 3 are correct.
(C) 2, 3, and 5 correct.
(D) 1 and 3 are
(E) 1,2, and 4 are incorrect.
11. Which of the following statements of electron transport chain is not true?
(A) Complex I contains copper ions.
(B) The final reduced species in the electron transport chain in mitochondria is H2O.
(C) The P/O ratio refers to the number of moles of ATP produced for each mole of oxygen atoms
consumed in electron transport.
(D) Cytochrome c is a macromolecule which is loosely bound to the outer surface of the inner
mitochondrial membrane.
(E) None of the above.
12. Which one of the following statements about glycolysis is correct:
(A) Conversion of glucose to glyceraldehyde-3-phosphate is an exergonic reaction.
(B) Conversion of glucose-6-phosphate to fructose-6-phosphate is a cleavage reaction.
(C) Conversion of glyceraldehyde-3-phosphate to give 1,3-bisphosphoglycerate is a dehydration
reaction.
(D) Conversion of dihyroxy acetone phosphate to glyceraldehyde-3-phosphate is an isomerization
reaction.
(E) Conversion of 2-phosphoglycerate to give phosphoenolpyruvate is an oxidation reaction.
13. 蛋白質中，含量最高之元素為：
(A) C, O, N, S (B) C, H, O, P (C) C, H, O, N (D) H, N, P, O.
14. 下列那一種不屬於 Protein 之機能？
(A) Catalysis
(B) Self-Replication
(C) Electron Transport
(D) Biological Regulation
15. 下列之 Amino Acids 中其 Side Chain 上, 帶有 Aromatic Group 的是：
(A) Leucine
(B) Serine
(C) Tyrosine
(D) Glutamine。
16. 下列哪一種 Amino Acid 分子量最小？
(A) Glycine
(B) Alanine
(C) Tyrosine
(D) Serine.
17. Iodination 發生在下列那一個胺基酸上？
(A) alanine
(B) tyrosine
(C) cysteine
(D) serine
(E) methionine
18. Threonine 之 Side Chain 上，帶有之 Group 為：
(A) Guanidine
(B) Hydroxy
19. Tryptophan 簡寫是： (A) T
(C) Thioether
(B) W
(C) Y
(D) Indole.
(D) P.
20.Tryptophan 之 side chain 上帶有之 group 為
(A) indole
(B) hydroxyl
(C)guanidine
(D) sulfhydryl
(E) imino.
21. Please point out which one of the following amino acids is covalently linked to the glycan moiety
of a glycoprotein molecule
(A) Trp
(B) Arg
(C) Gln
(D) Thr.
22. Which one of the following amino acids in a protein molecule cannot be modified by a protein
kinase？ (A)Tyr
(B)Thr
(C)Ser
(D)Trp.
23. Which amino acid is not produced by the precursor of 3-phosphoglycerate?
(A) serine
(B) glycine
(C) alanine
(D) cysteine
24. Branched hydrocarbon chains is not present in which of the following amino acids?
(A) valine
(B) lysine
(C) Leucine
(D) isoleucine.
25. 在 pH=7 的電泳(Electrophoresis)液中，通電後下列那種 Amino Acid 會往正極遊動？
(A) Serine
(B) Lysine
(C) Glutamic Acid
(D) Phenylalanine.
26. Histidine 之 side chain 的 pI 值為 (A) 5.6 (B) 6.0 (C) 7.6 (D) 8.6.
27. 在 UV280 nm 附近具有吸收峰之胺基酸為：
(A) WFK
(B) FYT
(C) FLI
(D) WFY.
28.Lysine 是一種
(A) acidic amino acid
(B)neutral amino acid
(C)basic amino acid
(D)aromatic amino
acid.
29. Collagen 和 Gelatin 中含有下列那一種 Amino Acid, 不存在於其它 Protein 中？
(A) Ornithine
(C) β-Alanine
(B) Citrulline
(D) 4-Hydroxy-proline
30. Protein 分子結構上那一種是屬於 Covalent bond？
(A) Disulfide bond
(B) Hydrogen bond
(C) Ionic bond
(D) Hydrophobic bond.
31. Dr. Pauling 所提出之 α-Helix 結構是屬於：
(A) Primary structure
(B) Secondary structure
(C) Tertiary structure
(D)Quaternary
structure.
32.蛋白分子之 α-Helix 結構中每個 Amino acid 之 Side Chain 都是：
(A)朝外
(B)朝內
(C)朝上
(D)朝下。
33.下列敘述何者為對？
(A)α-helix 為 interchain hydrogen bonding。
(B)α-helix 螺距為 5.4 aa/turn。
(C)β-structure 為 interchain hydrogen bonding。
(D)β-turn 是由 8 個胺基酸所組成。
(E)Collagen 含有很高量之 Ala 及 Pro。
34.在 Polypeptide 上下列那一種 Amino Acid 之出現最可能破壞其 α-helix 之構造：
(A) Proline
(B) Tyrosine
(C) Alanine
(D) Glutamine.
35. Triple helix 是那一種蛋白之特有構造？
(A) Hemoglobin
(B) Myoglobin
(C) Ribonuclease
(D) Collagen
36.蛋白之 β-Turn 結構是由幾個 Amino Acids 所構成？ (A) 4
(B) 5
(C) 7
(D) 8.
37.下列那一種酵素可把 Protein 中 Lys 和 Arg Residues 的 Peptide Bonds 打斷？
(A) Chymotrypsin
(B) Trypsin
(C) Thermolysin
(D) Pepsin
38.下列之 peptide (L-C-D-A-M-R-Q-G-P-T-M-S-V-L-N-K-F-I-T-A-K) 經 Chymotrypsin 酵素分解後可
得到幾段 peptide
(A) 1
(B) 2
(C) 3
(D) 4
(E) 5.5.
39.哪一種酵素可把 protein 中 Asp 及 Glu residues 的 peptide bonds 打斷？
(A) Pepsin
(B) Trypsin
(C) V8 protease
(D) Thermolysin.
40. Which of the following is not correct concerning 2,3-bisphosphoglycerate (BPG)?
(A) It binds at a distance from the heme groups of hemoglobin.
(B) It binds with lower affinity to fetal hemoglobin than to adult hemoglobin.
(C) It increases the affinity of hemoglobin for oxygen.
(D) It is an allosteric modulator.
(E) It is normally found associated with the hemoglobin extracted from red blood cells.
簡答題 (一律以英文作答, 胺基酸需寫全名)
1.在一條 polypeptide 中，可先以
胺基酸之 COOH 端切割 (2%)
把 cysteine 修飾，然後可由 cyanogen bromide 在
(註：胺基酸需寫全名)
2. Glycolysis is regulated by three enzymes catalyzing nonequilibrium reactions,
namely,
,
, and
. (3%)
3.There are at least seven types of glycosaminoglycans：
heparin sulfate,
, keratin sulfate (I and II).
, dermatan sulfate, heparin,
(2%)
4. 請問 Acetyl-CoA，nicotinamide adenine dinucleotide, reduced (NADH)，pyruvate
dehydrogenase(PDH) kinase，PDH phosphatase 對 PDH 活性的調控分別是抑制還是促進
(4%)
5. 主要提供 nucleotide 和 nucleic acid 合成所需的 ribose phosphate 是由那個代謝途徑產
生：
。 (1%)
6. 20 種 Protein Amino Acids 中那兩種 Amino Acids 含有兩個 Chiral Centers？那一種 Amino Acid 不
含 Chiral Center？
(2%)
7. Cysteine 的 side chain 上帶有何種 group
8. collagen 上含有高量的
。(1%)
，
，
三種氨基酸。 (3%)
9. Catalase (Mr=248,000), Ovalbumin (Mr=45,000)和 Ribonuclease (Mr=15,000)三者混合液, 經
Sephadex G-200 之 Gel Filtration Column Chromatography 後,三者流出之順序為何？
10.由許多 Protein Subunits 組成的立體結構稱為
structure
(1%)
(1%)
●以下為解答
1.(A)
2. (B)
3. (B)
4. (C)
5.(B)
6. (B)
7.(B)
8.(B)(C)(E)
9.(B)
10.(E)
11.(A)
12.(D)
13.(C)
14. (B)
15. (C)
16. (A)
17.(B)
18.(B)
19.(B)
20.(A)
21. (D)
22.(D)
23. (C)
24. (B)
25. (C)
26.(B)
27. (D)
28.(C)
29.(D)
30.(A)
31.(B)
32.(A)
33.(E)
34.(A)
35. (D)
36.(A)
37.(B)
38.(B)
39.(C)
40.(C)
簡答題
1.在一條 polypeptide 中，可先以 iodoacetate 把 cysteine 修飾，然後可由 cyanogen bromide 在
methionine 胺基酸之 COOH 端切割 (2%)
2. Glycolysis is regulated by three enzymes catalyzing nonequilibrium reactions, namely,
hexokinase , PFK-1 (或 phosphofructokinase-I) , and pyruvate kinase
. (3%)
3.There are at least seven types of glycosaminoglycans：
heparin, heparin sulfate, hyaluronate
chondroitin sulfate , dermatan sulfate,
, keratin sulfate (I and II).
(2%)
4. 請問 Acetyl-CoA，nicotinamide adenine dinucleotide, reduced (NADH)，pyruvate
dehydrogenase(PDH) kinase，PDH phosphatase 對 PDH 活性的調控分別是抑制還是促進
Acetyl-CoA
抑制
NADH
抑制
pyruvate dehydrogenase(PDH) kinase
抑制
PDH phosphatase
促進
5. 主要提供 nucleotide 和 nucleic acid 合成所需的 ribose phosphate 是由那個代謝途徑產生：
pentose phosphate pathway (PPP)
。
(1%)
6. 20 種 Protein Amino Acids 中那兩種 Amino Acids 含有兩個 Chiral Centers？那一種 Amino Acid 不
含 Chiral Center？
(1) threonine , isoleucine
(2) glycine
7. Cysteine 的 side chain 上帶有何種 group
thiol (SH) 。(1%)
8. collagen 上含有高量的 glycine ， alanine ， proline (或 hydroxyproline)
三種氨基酸。 (3%)
9. Catalase (Mr=248,000), Ovalbumin (Mr=45,000)和 Ribonuclease (Mr=15,000)三者混合液, 經
Sephadex G-200 之 Gel Filtration Column Chromatography 後,三者流出之順序為何？
Catalase, Ovalbumin, Ribonuclease
10.由許多 Protein Subunits 組成的立體結構稱為 quaternary
structure
(1%)
(1%)