Download Supplementary Figure. Confocal sections through a B cell that has

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Supplementary Figure. Confocal sections through a B cell that has ingested beads.
Arrow, nucleus. A-C, a
confocal section in the plane of the beadsb(two bright spots in A,
c
C). D-F, a plane through the apical surface of the cell. The beads are below this focal
plane, and above the basal surface, indicating they are within the cell. A and D show the
beads as well as SYBR Green staining of DNA. B, E: Alexa 633-Wheat Germ Agglutinin
(WGA) staining of cell membranes including nuclear envelope and vesicles. Note gaps in
staining where the beads lie in the cytoplasm. C, F: overlay of SYBR Green and beads
(green), f-actin stained with Alexa 568-Phalloidin (red), and WGA (blue). Bar, 5 microns
for all panels.
1
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