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Supplementary Figure. Confocal sections through a B cell that has ingested beads. Arrow, nucleus. A-C, a confocal section in the plane of the beadsb(two bright spots in A, c C). D-F, a plane through the apical surface of the cell. The beads are below this focal plane, and above the basal surface, indicating they are within the cell. A and D show the beads as well as SYBR Green staining of DNA. B, E: Alexa 633-Wheat Germ Agglutinin (WGA) staining of cell membranes including nuclear envelope and vesicles. Note gaps in staining where the beads lie in the cytoplasm. C, F: overlay of SYBR Green and beads (green), f-actin stained with Alexa 568-Phalloidin (red), and WGA (blue). Bar, 5 microns for all panels. 1