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From: Quantitative Analysis of Muscle Histologic Method in Rodent Facial Nerve Injury
JAMA Facial Plast Surg. 2013;15(2):141-146. doi:10.1001/jamafacial.2013.430
Figure Legend:
Figure 1. After Masson trichrome staining (MTS) of tissue sections containing dilator naris muscle and levator labii superioris
muscles, a sequential color deconvolution algorithm was applied, using Image J software, to measure muscle content of the
specimens, fiber surface area, and fiber counts. A through C, This example of a control specimen at ×20 magnification after MTS
demonstrated distinct 3-colored staining patterns. B, The entire specimen was then outlined with the Image J software. C, The
muscle area (red stain) was selectively outlined Copyright
through color-based
image
thresholding. D, The same specimen is shown at ×400
© 2013 American
Medical
Date
of download:
4/29/2017 muscle fiber staining is selectively outlined using the same thresholding approach.
magnification.
E, Individual
Association. All rights reserved.
From: Quantitative Analysis of Muscle Histologic Method in Rodent Facial Nerve Injury
JAMA Facial Plast Surg. 2013;15(2):141-146. doi:10.1001/jamafacial.2013.430
Figure Legend:
Figure 2. Mean maximal horizontal whisking amplitude at postrepair day 28. Recovery was significantly greater for the control side
(58.5°) compared with the experimental side (17.1°). Limit lines indicate 95% confidence intervals.
Date of download: 4/29/2017
Copyright © 2013 American Medical
Association. All rights reserved.
From: Quantitative Analysis of Muscle Histologic Method in Rodent Facial Nerve Injury
JAMA Facial Plast Surg. 2013;15(2):141-146. doi:10.1001/jamafacial.2013.430
Figure Legend:
Figure 3. Stereotypical features of muscle atrophy seen in dilator naris muscle and levator labii superioris on the experimental side.
A and B, Control side reveals healthy features of muscle fibers, including polygonal shape with overall uniform fiber size. C and D,
Experimental side reveals decreased volume of muscle replaced by collagen (blue stain) and adipose tissue (white space with
septation). Individual fibers display irregular round appearance (original magnification ×20 [A and C] and ×400 [B and D]).
Date of download: 4/29/2017
Copyright © 2013 American Medical
Association. All rights reserved.
From: Quantitative Analysis of Muscle Histologic Method in Rodent Facial Nerve Injury
JAMA Facial Plast Surg. 2013;15(2):141-146. doi:10.1001/jamafacial.2013.430
Figure Legend:
Figure 4. Quantitative comparison of muscle architectures. A and B, Statistically significant differences in muscle content and total
fiber count were noted between the control and experimental sides. C, The mean cross-sectional area of individual muscle fibers
was larger on the control side; however, this difference was not statistically significant. Limit lines indicate 95% confidence intervals.
Date of download: 4/29/2017
Copyright © 2013 American Medical
Association. All rights reserved.
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