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Dr. Rajesh Kumar
Assistant Professor (Biophysical & Bioinorganic Chemistry)
School of chemistry & Biochemistry,
Thapar University, Patiala – 147 004
Research Interest:
Spectroscopic, Kinetics, Thermodynamics, and Structural Studies of Proteins and Enzymes.
Protein Folding, Misfolding, and Conformational Diseases
Ultrafast Protein Folding Dynamics
Iron Metabolism, Storage, and Transport Proteins
1.
2.
3.
4.
Education:
Sr. No.
University/Institute
Degree Awarded
Year of
Completion
Degree and Specialization
1.
School of Chemistry,
University of Hyderabad
M.Phil.
2001
Chemistry
2.
School of Chemistry,
University of Hyderabad
Ph.D.
2006
Biophysical and
Bioinorganic Chemistry
Thesis Title: “Effects of Chaotropic Salts and Cosolvents on the Stability, Folding, and Dynamics of
Proteins” Thesis supervisor: Prof. Abani K. Bhuyan
Research and Teaching Experience
Sr. No.
Institution/Originations/Country
1.
Thapar University, Patiala
2.
3.
The University Of British
Columbia, Vancouver,
Canada
Position
Assistant Professor
Canadian Institutes Health
Research-Canadian Blood
Services, Post-Doc Fellow,
Mentor: Prof. Grant A.
MauK (MD, PhD, Canada
Research Chair in
Transfusion Science)
University of Hyderabad (Central
University)
INDIA
Research Fellow
From
(date)
July, 2006
To (date)
Till date
Jan, 2007
Jan 2009
Feb., 2006
July, 2006
Professional recognition, awards, fellowships received
Duration
Sr. No.
Institution/Originations/Country
Honor
1.
Canadian Institutes of Health Research/
Canadian Blood Services
Personal Research
Award in Area of
Transfusion Science
2006-2010
(Spring 2006)
2.
School of Chemistry, University of Hyderabad
SRF (DBT)
2002-2005
3.
School of Chemistry, University of Hyderabad
SRF (DST)
2005-2006
4.
School of Chemistry, University of Hyderabad
Research Assistantship
2001-2002
Ongoing/completed sponsored projects:
S. No.
1.
2.
3.
4.
Title
Sugars and salts-induced
folding and stabilization of
base-denatured horse
cytochrome c
Kinetic and thermodynamics
studies on the effect of
chaotropic and kosmotropic
coslovents on horse
ferrocytochrome c
The kinetics and mechanism
of iron release from
transferrins
Project
cost
(lacs)
31.0
Funding Agency
Indian Council of Medical
Research (ICMR)
21.3
CSIR, Govt. Of India
18.2
DST, Govt. Of India
pH-dependent stability and
11.5
microsecond-folding kinetics
of horse ferrocytochrome c
UGC, Govt. Of India
5.
The mechanism of iron
release from human
transferrin
50
Canadian Blood ServicesCanadian Institutes of
Health Research, Canada
6.
The mechanism of reductive
release of iron from serum
transferrin
34
Indian Council of Medical
Research (ICMR).
Scheme
Duration
Ad-hoc
research
project
(approved)
Extramural
research
project
(Ongoing)
2013-2015
2-years
Young
scientist fast
track
(Completed)
Major
Research
Project
(Ongoing)
CIHR/CBSTransfusion
Science
(Completed)
Ad-hoc
research
project
(approved)
(20102013)
3-years
2011-2014
3-years
2007-2009
(2-years)
3-years
2013-2016
Research publications in international referred journal
1.
Kumar, S., Sharma, D., Kumar, R., and Kumar, R. (2014) Electrostatic Effects Controls the Stability and Iron
Release Kinetics of Ovotransferrin J. Biol. Inorg. Chem. (accepted on 29th April) (IF 3.4)
2.
Kumar, S., Sharma, D., and Kumar, R. (2014) Effect of Urea and Alkylureas on the Stability and
Structural Fluctuation of the M80-Containing Ω-loop of Horse Cytochrome c. Biochimica et Biophysica
Acta, 1844, 641–655. (IF 3.75)
3.
Jain, R., Kaur, S., and Kumar, R. (2013) Guanidine hydrochloride-induced alkali-molten globule model of
horse ferrocytochrome c. Journal of Biochemistry, 153, 161-177. (IF 2.73)
4.
Jain, R., Sharma, D., and Kumar, R. (2013) Effects of Alcohols on the Stability and Low-Frequency Local
Motions that Control the Slow Changes in Structural Dynamics of Ferrocytochrome c. Journal of
Biochemistry, 154, 341-354 (IF 2.73).
5.
Kumar, R. Jain, R., and Kumar, R. (2013) Viscosity-Dependent Structural Fluctuation of the M80-Containing
Ω-Loop of Horse Ferrocytochrome c. Chemical Physics, 418, 57-64. (IF 2.0).
6.
Kumar, R., Sharma, D., Kumar, S., and Kumar, R. (2014) Factor defining the effects of glycine betaine on the
thermodynamic stability and internal dynamics of horse cytochrome c. Biochemistry (ACS revision
requested- bi-2014-00356c, IF 3.4).
7.
Kumar, R., Sharma, D., Kumar, S., and Kumar, R. (2014) Structural, kinetic, and thermodynamic
characterizations of SDS-induced molten globule state of a highly negatively charged cytochrome c. Journal
of Biochemistry (revision requested- JB-14-02-0031, IF 2.73)
8.
Jain, R., and Kumar, R. (2013) Analysis of the pH-Dependent Stability and Folding Kinetics of Horse
Cytochrome c (2014). Proteins: Structure, Function, and Bioinformatics (under review: Prot-00196-2014, IF
3.35).
9.
Kumar, R. and Mauk, A. G. (2012) Protonation and Anion Binding Control the Kinetics of Iron Release from
Human Transferrin. J. Phys. Chem. B (ACS), 116, 3795-3807. (IF 3.6) (cited by 1)
10. Kumar, R. and Mauk, A. G. (2009) Atypical Effects of Salts on the Stability and Irons Release Kinetics of
Human Serum Transferrin. J. Phys. Chem. B., 113, 12400-12409. (IF 3.6) (cited by 8)
11. Kumar, R. and Mauk, A. G. (2009) Iron Release from Human Transferrin in the Absence of Chelators
Involves Five Kinetic Steps at Acidic pH. J. Biol. Inorg. Chem.14, S185-S224. (IF 3.4)
12. Kumar, R. and Bhuyan, A. K. (2009) Entropic Stabilization of Myoglobin by Subdenaturing Concentrations
of Guanidine Hydrochloride. J. Biol. Inorg. Chem., 14, 11-21. (IF 3.4) (cited by = 3)
13. Kumar, R. and Bhuyan, A. K. (2008) Viscosity Scaling for the Glassy Phase of Protein Folding. J. Phys.
Chem. B 112, 12549-12554. (IF 3.6); cited by = 8)
14. Yadaiah, M., Kumar, R., and Bhuyan, A. K. (2007) Glassy dynamics in the folding landscape of cytochrome
c: Detected by laser photolysis. Biochemistry, 46, 2545-2551. (IF 3.4) (cited by = 7)
15. Kumar, R. and Bhuyan, A. K. (2007) Effect of guanidine hydrochloride on stability and dynamics of
myoglobin. J. Biol. Inorg. Chem., 112, S223-S230. (IF 3.4)
16. Kumar, R. Prabhu, N. P. Rao, D. K, and Bhuyan, A. K. (2006) The Alkali Molten Globule State of Horse
Fericytochonne c: Observation of Cold Denaturation. J. Mol. Biol., 364, 483-495. (IF 4.0) (cited by = 21)
17. Rao, D. K, Kumar, R. Yadaiah, M., and Bhuyan, A. K. (2006) The Alkali Molten Globule State of
Ferrocytochrome c: Extraordinary Stability, Persistent Structure, and Constrained Overall Dynamics.
Biochemistry, 45, 3412-3420. (IF 3.4) (cited by = 10)
18. Kumar, R., Prabhu, N. P., and Bhuyan, A. K. (2005) Ultrafast Events in the Folding of Ferrocytochrome c
Biochemistry, 44, 9359-9367. (IF 3.4) (cited by = 14)
19. Kumar, R., and Bhuyan, A. K. (2005) Two-state Folding of Horse Ferrocytochrome c: Analyses of Linear
Free Energy Relationship, Chevron Curvature, and Stopped-Flow Burst Relaxation Kinetics. Biochemistry,
44, 3024-3033 (cited by = 13) (IF 3.4)
20. Prabhu, N. P., Kumar, R., and Bhuyan, A. K. (2004) Folding barrier in horse cytochrome c: support for a
classical folding pathway. J. Mol. Biol., 337, 195-208. (IF 4.0) (cited by = 19)
21. Kumar, R., Prabhu, N. P., Yadaiah, M., and Bhuyan, A. K. (2004) Protein stiffening and entropic stabilization
in the subdenaturing limit of guanidine hydrochloride. Biophysical J., 87, 2656- 2662. (IF 3.7) (cited by = 32)
22. Bhuyan, A. K.; Kumar, R. (2002) Kinetic barriers to the folding of horse cytochrome c in the reduced state.
Biochemistry, 41, 12821-12834. (IF 3.4) (cited by = 27)
Publications in national and international conferences:
1.
Jain, R., Sharma, D., and Kumar, R. (2012). Thermodynamic Analysis of Effect of Alcohols on Thermal Stability
of Horse Ferrocytochrome c. International Symposium on Protein Folding and Dynamics being organized at
NCBS, Bangalore.
2.
Jain, R., and Kumar, R. (2012) Guanidine Hydrochloride-Induced Folding and Stabilization of Alkali-Denatured
Carbonmonoxycytochrome c. International Symposium on Protein Folding and Dynamics being organized at
NCBS, Bangalore.
3.
Kumar, S., and, Kumar, R. (2011) Iron Release from Diferric Ovotransferrin in the Absence of Chelators Involves
Six Kinetic Steps at Acidic pH. 6th National Conference on Thermodynamics of Chemical and Biological
Systems, Department of Chemistry, Maharshi Dayanand University, Rohtak,
4.
Kumar, R., Kumar, S., and Kumar, R. (2012) Effect of neutral salts on the stability of acid-denatured hen egg
white lysozyme . Material Research Society of India (23 rd Annual meeting, Thapar University, 143.
5.
Kumar, S., Kumar, R., and Kumar, R , (2012) Atypical effect of salts on the stability of Fe 3+-ovotransferrin CO32complex Material Research Society of India, 23rd Annual meeting, Thapar University,134.
6.
Jain, R., and Kumar, R. (2012) Entropic stabilization of Ferrocytochrome c by subdenaturing concentrations of
methanol. Material Research Society of India, 23rd Annual meeting, Thapar University,136
7.
Kaur, S., Jain, R., and Kumar, R. (2011) Effect of Osmolytes on the Stability and Motional Dynamics of Horse
Ferrocytochrome c. (Advances in Chemical Engineering, Macmillan Advanced Research Series, 137-143.
8.
Jain, R., and Kumar, R. (2011) Effect of neutral salts on the stability and dynamics of horse cytochrome c
National Conference on Emerging Trends in Chemistry-Biology Interface, Kumaun University, Nainital.
Teaching
Course Taught
General Chemistry and Chemistry lab
UG/PG
(UG)
Quantum Chemistry
PG (Chem)
Electrochemistry and Thermodynamics
Advanced Topics in Chemistry
Protein Chemistry
Molecular Spectroscopy
Biomolecular Chemistry
Biophysics and Biostatistics
PG (Chem)
PG(Chem.)
PG(Chem.)
PG(Chem.)
PG(Biotech)
Year
2006, 2007, 2009,
2010, 2011, 2012
2009, 2010, 2011,
2012
2011, 2012, 2013
2011, 2012, 2013
2011, 2012
2010, 2012
2009
2009
Institute/ University
Thapar University
Thapar University
Thapar University
Thapar University
Thapar University
Thapar University
Thapar University
Thapar University
Research guidance
S.No.
1.
2.
Name of
Student
Ms. Rishu
Jain
Mr. Rajesh
Kumar
Level
PhD/MSc
PhD
PhD
Title of Thesis
Effect of chaotropic salts and
denaturants on the stability, folding,
and dynamics of proteins
Kinetic and thermodynamic studies on
effect of amino acids, alcohols,
crowding agents, and lyotropic salts on
proteins
Year of
registration
Ongoing/
completed
2011
Thesis
Submitted
2011
Ongoing
3.
Mr.
Sandeep
Kumar
Mr.
Sanjeev
Kumar
Ms.
Karamjeet
Kaur
Ms. Deeraj
Rani
Ms. Rumit
PhD
Ms.
Gurinder
Pal Kaur
MSc.
9.
Ms. Rina
Joshi
10.
Ms.
Rajvinder
Kaur
4.
5.
6.
7.
8.
2011
Ongoing
2012
Completed
MSc.
Effect of neutral salt on stability and 2009
dynamics of horse ferrocytochrome c,
Completed
MSc.
Effect of salts on the thermal stability 2011
of horse ferricytochrome c at acidic pH
Guanidine hydrochloride - induced 2009
folding and stabilization of alkali
denatured
state
of
horse
ferrocytochrome c,
Effect of solvent viscosity on the 2009
motional
dynamics
of
native
carbonmonoxy-cytochrome c
Completed
MSc.
Effect of amino acids on the thermal 2013
stability of hen egg white lysozyme
Completed
MSc
Effect of sugars on the thermal 2013
stability of hen egg white lysozyme
Completed
MSc.
MSc.
Effect of weak denaturants, sugars,
salts, and metals ions on stability,
folding, and dynamics of proteins.
Effect of salts on the thermal stability
of hen egg white lysozyme
Completed
Completed