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Department of Chemistry and Physics
Biochemistry/Lab
Last Date Revised: 10/12/2016
I. COURSE NUMBER AND TITLE:
CHEM 3650 DA1 Biochemistry/Lab
Fall 2016 DA1 20465
Tuesday, 08/23/2016 - Tuesday, 12/06/2016
Lecture-main campus TR 1:00 PM - 2:15 PM
See location at https://www.fcas.nova.edu/coursewizard/
Lab-Parker 113 (wet lab) T 2:30 PM - 5:15 PM
II. INSTRUCTOR:
Dr. Reza Razeghifard
Email: [email protected]
Office Hours: Tues. 10am-12am, Thurs. 10am-11am
Office Location: Parker-351. Phone: 954-262-7930
III. COURSE DESCRIPTION:
The chemical properties of amino acids, monosaccharides, lipids and nucleotides are
discussed. The structure of proteins, carbohydrates and biological membranes are studied.
Mechanisms of enzymatic catalysis are outlined in detail with an emphasis on the
structure/function of cofactors. Glycolysis and citric acid cycle are described. Electron
transport and ATP synthesis are discussed in both mitochondria and chloroplasts.
Metabolism of lipids, amino acids and nucleotides are presented. In addition to mechanistic
studies of biochemical pathways and cycles, regulation of these processes is also covered.
Prerequisites: BIOL 1500 and CHEM 2200 or CHEM 2410 or CHEM 2410H.
IV. LEARNING OUTCOMES:
Upon the completion of the course, the student is expected to be able to:
1. Discuss the key molecular reactions involving the mechanistic studies of
biochemical pathways and processes;
2. Develop problem-solving skills, critical thinking and analytical reasoning in the
field of biochemistry;
3. Demonstrate proper procedures for handling chemicals and utilizing
instrumentation used in biochemistry;
4. Develop skills to carry out biochemical protocols, collect and analyze the data;
5. Critically assess a biochemistry topic by reading relevant current research articles
found and accessed through electronic scientific databases.
V. REQUIRED TEXTS AND MATERIALS:
Fundamentals of Biochemistry: Life at the Molecular Level
Author: Voet and Pratt
Publisher: Wiley
Year: 2016; Edition: 5th
Fundamental Laboratory Approaches for Biochemistry and
Biotechnology Author: Ninfa, Balou, Benore
Publisher: Wiley
Year: 2010; Edition: 2nd
ISBN: 9781118918401
ISBN: 9780470087664
VI. COURSE REQUIREMENTS AND POLICIES:
Students are expected to:
Attend class (attendance recorded).
Take good class notes.
Read assigned material before class and be prepared for class questions and discussion.
Attend lab sessions and submit timely and well-written lab reports. Every student must
write their own lab reports even though they have worked with a lab partner.
Contact the instructor if you are having problem.
Attend help sessions if required by the instructor.
Study in groups and review the course material and quiz each other.
Display college-level writing ability in written lab reports, exams and homework.
Observe all laboratory safety precautions and procedures.
No cell phones or laptops are allowed in class or lab.
Academic dishonesty (cheating, plagiarism, etc.) on exams and reports will be dealt with harsh
penalty, at minimum, with a failing grade.
It is the student responsibility to take all exams and attend all lab sessions on their scheduled
dates. Failure to do so will result in a zero for that exam or that lab session unless your absence
is due to special circumstances beyond your control.
“Partners in the lab can only share the data they jointly produce, but each student needs
to complete the lab report, including data analysis and report writing, independently and
individually. Producing the lab report in any joint effort or fashion by lab partners will
be considered plagiarism.”
Students with ADA accommodations (see clause g under IX: COLLEGE-WIDE POLICY
STATEMENTS) must make their testing appointments at least two weeks prior to the exam
dates.
VII. COURSE SCHEDULE AND TOPIC OUTLINE:
Tentative Lecture Schedule
Day
Date
Topic
Aug. 23
T
Amino Acids and Proteins: Primary Structure
Aug. 25
R
Proteins: Primary Structure
Aug. 30
T
Proteins: 3-Dimensional Structure
Sept. 1
R
Proteins: 3-D Structure and Protein Function
Sept. 6
Proteins: 3-D Structure and Protein Function and
T
Carbohydrates
Ch
4,5
5
6
7
7, 8
Sept. 8
Sept. 13
R
Sept. 15
Sept. 20
R
Sept. 22
Sept. 27
Sept. 29
Oct. 4
Oct. 6
Oct. 11
Oct. 18
Oct. 20
Oct. 25
Oct. 27
Nov. 1
Nov. 3
Nov. 8
Nov. 10
Nov. 15
Nov. 17
Nov. 22
Nov. 29
Dec. 1
T
T
R
T
R
T
R
T
T
R
T
R
T
R
T
R
T
R
T
T
R
T
Dec. 6
Carbohydrates and Lipids and Biological Membranes
Lipids and Biological Membranes and Membrane
Transport
Exam I (CH 4-10)
Enzymatic Catalysis and Enzyme Kinetics, Inhibition
and control
Enzyme Kinetics, Inhibition and control and
Biochemical Signaling
Biochemical Signaling
Introduction to Metabolism and Glucose Catabolism
Glucose Catabolism
Review
Mid-Term Exam (CH 11-15)
Glycogen Metabolism & Gluconeogenesis
Gluconeogenesis and Citric Acid Cycle
Citric Acid Cycle
Electron Transport and Oxidative Phosphorylation
Electron Transport and Oxidative Phosphorylation
Photosynthesis
Photosynthesis
Exam II (CH 16-19)
Lipid Metabolism
Amino Acid Metabolism
Nucleotide Metabolism
Mammalian Fuel Metabolism: Integration &
Regulation
ACS Final Exam (comprehensive) (8:00am-10:00am)
for location see
https://www.fcas.nova.edu/coursewizard/
8, 9
9, 10
11, 12
12, 13
13
14, 15
15
16
16, 17
17
18
18
19
19
20
20, 21
23
22
Laboratory Schedule
FR: Full Report - SR: Short Report
Week
Aug. 22
Aug. 29
Sept. 5
Sept. 12
Lab
Chapter/Exp
Safety + Excel SR
Internet Resources and Software for Protein
Biochemistry SR
Drug Metabolism by Rat Microsomes
Protein Purification Using Ion-Exchange
Chromatography FR Part I
Sept. 19
Native Protein Electrophoresis (protein pI) FR
Part II (including Part I from previous lab)
Sept. 26
Protein Electrophoresis (SDS-PAGE) SR
Oct. 3
Enzyme kinetics of β-Galactosidase FR
Oct. 10
Oct. 17
Midterm Exam week - No labs
Enzymatic determination of glucose
concentration SR
Western Blot to Identify His-tag Thioredoxin
FR
Lipid lab
Activity Measurements of Plant Chloroplasts
and HPLC of Plant Pigments SR
Oct. 24
Oct. 31
Nov. 7
Chapter 2 / Exp 2.6 Page
51-61
Chapter 15 /Exp 2.6 Page
440
Handout
Chapter 5 / 5.4, Chapter 7
/ 7.11 and Chapter 9 / 9.4
Chapter 6 / 6.2 Page 163
Chapter 6 / 6.3 Chapter 4
/ 4.4
Chapter 10 /10.3 and
Chapter 5 / Exp 5-2
Chapter 12 / Exp 12-1
and Exp 12-4
Chapter 6 / 6.5 and
Handout
Handout
Chapter 8/ Exp 8-1 and
Handout
VIII. GRADING CRITERIA:
Exam I and II (12% each)
Mid-Term Exam
Final Exam
Labs (total)
Assignments
26%
13%
30%
25%
6%
Grading Scale:
A
93.3-100
A90.0-93.2
B+
86.6-89.9
B
83.3-86.5
B80.0-83.2
C+
76.6-79.9
C
CD+
D
F
73.3-76.5
70.0-73.2
66.6-69.9
60.0-66.5
<60.0
IX: COLLEGE-WIDE POLICY STATEMENTS
Students should visit www.fcas.nova.edu/about/policies.cfm to access additional
required college-wide policies. It is your responsibility to access and carefully read
these policies to ensure you are fully informed. As a student in this class, you are
obligated to follow these college-wide policies in addition to the policies established
by your instructor.
The following policies are described on this website:
 Academic misconduct
 Last day to withdraw
 Email policy



Student course evaluations
Student responsibility to register
Student responsibility for course prerequisites
Additional Academic Resources: Nova Southeastern University offers a variety of
resources that may aid in student success. Among these resources are:
Accommodations for students with documented disabilities: For more information
about ADA policy, services, and procedures, students may call the Office of Student
Disability Services at 954-262-7189 or visit http://www.nova.edu/disabilityservices.
Tutoring and Testing Center: Free tutoring services: Students are encouraged to
use the free, individualized tutoring services offered by the Tutoring and Testing Center
(TTC). TTC provides a supportive atmosphere in which tutors and students work
collaboratively on improving students’ writing, math and/or science skills. To set up a
tutoring appointment, call (954) 262-8350 or (800) 541-6682, ext. 28350 or stop by the
Tutoring and Testing Center in the Student Affairs Building, 2nd floor. For additional
resources, as well as information about tutoring at the regional campuses visit the
Tutoring and Testing Center website at http://www.nova.edu/tutoringtesting/index.html