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THE VIB-UGent TRANSMISSION ELECTRON MICROSCOPY (TEM)
CORE FACILITY
Riet De Rycke 1,2,3,4, [email protected]
Michiel De Bruyne 1,2,3,4 [email protected]
1. Inflammation Research Centre (IRC), VIB, Ghent, Belgium and Department of Biomedical Molecular Biology, Ghent University, Technologiepark 927, 9052 Ghent, Belgium. http://www.irc.ugent.be
2. Department of Plant Systems Biology (PSB), VIB, Ghent, Belgium and Department of Plant Biotechnology and Bioinformatics, Ghent University, Technologiepark 927, 9052 Gent, Belgium. http://www.psb.ugent.be
3. Medical Biotechnology Center (MBC), VIB, Ghent, Belgium, Ghent University, A. Baertsoenkaai 3, 9000 Gent, Belgium. http://www.mbc.ugent.be
4. Bio Imaging Core (BIC), Technologiepark 927, 9052 Gent, Belgium https://corefacilities.vib.be/bic#services
THE UGENT-VIB TRANSMISSION ELECTRON MICROSCOPY CORE FACILITY is located on the campus ‘INW’ of the UGent and is providing expertise in the use and application of transmission electron
microscopy to serve biological or biomedical research. The facility was established in 2003 and shares the equipment of the Department of Biology. The aim of the facility is to support research projects of
many scientific groups of IRC, PSB, MBC and other research groups of Ghent University
MA (Morphological analysis) Embedding medium : Spurr’s resin
IEM (Immuno-gold Electron Microscopy) Embedding medium : LR-White
Chem fix : chemical fixation HPF : High Pressure Freezing
xylem region
WT
interfascicular fibers
WT
MA Chem fix
5µm
5µm
MA Chem fix
MA Chem fix
Vanholme et al.
The Plant Journal, 2010
Small intestinal crypt region
WT
A20IEC/myel-KO
MA HPF
in A. thaliana roottip
IEM HPF anti-GFP/aquaporin
in A. thaliana roottip
interfascicular fibers
comt C4H:F5H1
xylem region
comt C4H:F5H1
2µm
2µm
5µm
5µm
Disturbed ER in
XBP-1-deficient
CD8α+ DC’s
Engineering traditional monolignols out of lignin
by concomitant up-regulation of F5H1 and downregulation of COMT in Arabidopsis
Osorio et al.
Nature Immunology, 2014
4µm
4µm
IEM HPF anti-GFP/PAT2
in lytic vacuoles of
roottip A.thaliana
Paneth cell Stem cell
2µm
condensed
phagocytosed dead cell remnants
The unfolded-protein-response sensor IRE-1α
regulates the function of CD8α+ dendritic cells
Arabidopsis SNAREs SYP61 and SYP121 coordinate the
trafficking of plasma membrane aquaporin PIP2;7 to
modulate the cell membrane water permeability
Hachez et al.
Plant Cell, 2014
Vacuole
PVC
Nu
lipid droplet
Vereecke et al.
Nature Communications, 2014
2µm
MMP8-Dependent Extracellular Matrix
Cleavage at the Blood–CSF Barrier Contributes to Lethality
during Systemic Inflammatory Diseases
MA Chem fix
IEM Chem fix
Feraru et al.
The Plant Cell, 2010
Vandenbroucke et al.
EMBO Molecular Medicine, 2012
2S-albumin in PVC’s
De Meyer et al.,
Plant biotechnology Journal, 2015
Cell wall
Mv
MMP13+/+
Nu
8hLPS
MMP13-/-
IEM HPF with VHH28Fc
as primary antibodies in
A. thaliana Col seed
8hLPS
ER
2µm
cytoplasma
Tongda Xu et al.
Science, 2014
Mv
CSF
BM
Mv
BM
Nu
200nm
200nm
2µm
6µm
2µm
0.3μm
MMP13 contributes to tight junctions
destabilization
Vandenbroucke et al.
The Journal of neuroscience, 2012
Label in ER
Label on cell surface
0.3μm
MA Chem fix
IEM HPF anti-GFP/ABP1
in A. thaliana roottip
HPF MA A.thaliana roottip
Nodszynski et al.
Molecular Plant 2013
MA Chem fix
De Groote et al.
Cell Death and Differentiation, 2015
FADDODC-KO
Oligodendrocyte-Specific FADD
Deletion Protects Mice from
Autoimmune-Mediated Demyelination
Multilayered
PVC
Retromer Subunits VPS35A and
VPS29 Mediate Prevacuolar
Compartment (PVC) Function in
Arabidopsis
1µ
m
1µ
m
Mouse corpus callosum
myelination
Loricrin
Multilayered
PVC
FADDFL/FL
Mc Guire et al.
The Journal of Immunology, 2010
Pat3 mutant shows defects in late endocytic pathway
RIP4 deficient skin (E18.5) shows patches of ‘internal cornification’
MVB
MA HPF
400nm
GA
MA Chem fix
Sanne Dhondt-Fransiska MalfaitSophie Janssens-Anne De Paepe
Vandewynckel et al.
Hepatology International, 2014
MA Chem fix
GA
Swollen ER
Normal ER
Naramoto et al.
Plant Cell, 2014
DEN-induced HCC
2µm
Dilated ER
Reorganisation
of ER
ctrl
MUT
Vasc.Ehlers-Danlos Syndrome
Mouse skin male
30μm
Modulation of the unfolded protein response impedes
tumor cell adaptation to proteotoxic stress: a PERK
for hepatocellular carcinoma therapy
Intestinal metaplasia in an orthotopic
Vandewynckel et al.
mouse model
Digestive and Liver
for hepatocellularcarcinoma
30μm
Disease, 2014
MA Chem fix
ctrl
Nu
Lu
Lu
m
JC
BB
5µm
30µ
m
livercell
Enterocytes
Basolateral
Membrane
(infolded)
Lu: lumen
BB: brush border
(microvilli)
Nu: nucleus
JC: junctional
complex
m: mitochondria
35S:GRF5
HAM
VAN
TIP
30μm
3µm
35S:GRF5
ctrl
3µm
30μm
Growth regulating factor5 stimulates
At leaf chloroplast division
Brackman et al.
Scientific reports, 2016
3µm
sucr
COMB
3µm
Differences in chloroplast morphology,
number and size in the tip and base of
sucrose-treated and control leaves
Vercruyssen et al.
Plant Physiology, 2015
Ctrl: control
HAM: hamamelitannin
VAN: vancomycin
COMB: combination
HAM and VAN
sucr
BASE
2µm
TIP
DEN-induced HCC Tunicamycin
Mouse liver
normal collagen
Dilated
collagen
MA Chem fix
Col-0
BASE
MUT
Van Dingenen et al.
Plant Physiology, 2016
MA Chem. fix.
fibroblast
1802
fibroblast
Abnormal morphology or distribution of TGN/EE is
observed in gnom mutants
MA Chem. fix.
Col-0
WT
5µm
500nm
van7
vesiculated TGN/EE.
Saline
2µm
500nm
WT
Negative staining
Zhang et al.
ChemicalCommunications, 2014
500nm
Effect of treatment on lysostaphin susceptibility and thickness of the cell wall
on Staphylococcus aureus biofilms
200nm
influenza virus
200nm
virus mixed with
a-2,6-sialyllactose
glyco-goldNP
200nm
virus mixed with
lactose glyco-goldNP
Influenza-binding sialylated
polymer coated gold nanoparticles
prepared via RAFT polymerization
and reductive amination
For more info : http://users.ugent.be/~rderycke/