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Mattioli Engineering
Dermoelectroporation®: the
only one US FDA approved
alternative to injection
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation®
The only top No.1
technology alternative to
injections
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation®

Dermoelectroporation® is a technology that allows
product delivery into the various layers of the skin or
subcutaneous tissues.

Any water soluble ingredients can be inserted into the skin
without additional carrying Gels.

Dermoelectroporation® utilizes the skin water based
channels to allow product penetration due to
controlled current pulses delivered to the patient.
Copyright 2008 © Mattioli Engineering All rights reserved
Why Dermoelectroporation®

Mattioli Engineering microdermabrasion leader since
1991

Empowering Skin Care specialists across the world with
revolutionary technologies to treat skin.

Additional opportunities to further enhance delivery of
active ingredients into the skin was sought.

Combining the concept of Meso therapy (by Dr. Pistor,
1952) and Mattioli Engineering’s technical expertise led to
development of Dermoelectroporation.
Copyright 2008 © Mattioli Engineering All rights reserved
What Dermoelectroporation is not
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Microdermabrasion
Galvanic
Ionthophoresis
Ultrasound / Sonophoresys
Aquaphoresys
Massagers
Laser device
Transderm® is the ONLY and REAL alternative to injections.
Copyright 2008 © Mattioli Engineering All rights reserved
Existing Technologies for delivery
Microdermabrasion
Medical and Aesthetic application
– 1st transdermal pathway
– Skin surface action only
– No current applied
– Small sized molecules only
– Low delivery rate
Copyright 2008 © Mattioli Engineering All rights reserved
Existing Technologies
Traditional Galvanic (1to 5mA)
Aesthetic application
– Pushes ions into the skin.
– Low Dc current level applied.
–
–
–
–
–
Ineffective for high weight molecules.
Pre-charged compounds needed.
Small sized molecules only.
Substance PH will change.
Electrolysis (product separation).
Low Dc current level
applied
Copyright 2008 © Mattioli Engineering All rights reserved
Existing Technologies for delivery
Traditional Pulsed Ionthophoresis (1to 5mA)
Medical and Aesthetic application
– Pushes ions into the skin.
– Low Ac current level applied.
–
–
–
–
–
Ineffective for high weight molecules.
Pre-charged compounds needed.
Small sized molecules only.
Substance PH may change.
Electrolysis (product separation).
Sinus Low Ac current
level applied
Alternative Low Ac
current level applied
Copyright 2008 © Mattioli Engineering All rights reserved
Existing Technologies for delivery
Electroporation
In lab application only
High peak
currents
– Macromolecules delivery
– High peak currents needed
to porate the external skin
layers (18,000volts)
– High risk of permanent
skin damage
– Many Adverse effects
Copyright 2008 © Mattioli Engineering All rights reserved
Ionthophoresys
currents
Existing Technologies for delivery
Ultrasounds
Medical and Aesthetic application
– No current applied
– High vibration frequency on the skin
– Ineffective for high weight molecules
– Small sized molecules only
High Frequency
vibration 3MHz or
higher
Copyright 2008 © Mattioli Engineering All rights reserved
Existing Technologies for delivery
Sonophoresys (Sound)
Medical and Aesthetic application
– Low frequency Ultrasound
– Ineffective for high weight molecules
– Small sized molecules only
High Frequency
vibration Lower than 3
MHz
Copyright 2008 © Mattioli Engineering All rights reserved
Existing Technologies for delivery
Aquaphoresys (1to 5mA)
Medical and Aesthetic application
– Ultrasounds + current stimulation
– Low Ac current
– High freq. Ultrasounds
–
–
–
–
Ineffective for high weight molecules
Small sized molecules only
Ionic drug solutions PH may change
Electrolysis (ionic separation)
Low Ac current level
applied
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation®

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Is a Proprietary Patented Technology from Mattioli Engineering
Engineering.
The only US FDA Cleared Device as alternative to injections (Top n.1)
Micro-molecules and MACRO-molecules are safely delivered.
Using the skin’s water based “channels” (aquaporins).
Low and controlled current pulses delivered to the skin
No skin damage, no adverse effects, no contraindications
No effect on the substance being inserted/”injected”.
Faster than traditional Ionthophoresis / Galvanic devices (same
amount of product)
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation®
What the Technology is
used for?
Copyright 2008 © Mattioli Engineering All rights reserved
FOR EDUCTIONAL PURPOSES ONLY
Transderm® Meso® System®
Dermatology
treatments.
Skin rejuvenation and bio revitalization.
Cellulite treatments.
Fat deposits
HyperHidrosis (Botulinum toxine administration)
Acne pathologies.
Hormone replacement treatments
Pre laser treatments.
Pre and post plastic surgery sessions.
Replacing injections for Mesotharapy applications.
Copyright 2008 © Mattioli Engineering All rights reserved
FOR EDUCTIONAL PURPOSES ONLY
Transderm® Meso® System ®
Meso-Botulinum
Toxine applications.
Wrinkles treatments.
Stretch mark treatments.
Actinic Keratosis applications.
Steroids applications
Pain management
Rheumatic and post rheumatic pathologies.
Sports medicine (F.C. Juventus)
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation®
Who is this Top No.1
technology available for?
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation®
Dermoelectroporation® is available for:
Skin Care Specialists that want to optimize the delivery of a
high range of ingredients and not only a very few of
them…..
Clinics/salons that realize the limitation of conventional
beauty treatments and want to offer additional treatments
with a significant improvement in efficacy to move ahead
off common level clinics / salon….
..Providing a wide range of applications in only one device
and/or improving existing protocols on use.
Copyright 2008 © Mattioli Engineering All rights reserved
Mattioli Engineering’s Solution
Scientific principle
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation®
Dermoelectroporation® utilizes the skin water based
channels to allow ionic drug solutions to penetrate due to
controlled current delivered to the patient.
(Dermoelectroporation® stimulates the opening of the existing water based channels to
create a transdermal passage to deliver drugs deep into the derma.)
Copyright 2008 © Mattioli Engineering All rights reserved
Scientific principle
-Reproduces transient changes in skin microstructure
-Current flowing through the extra cellular matrix
(mainly composed by water, ions, and glycosaminoglycans) to make the
-Long-chained glycosaminoglycans to ,transiently, orient themselves to
create “channels” (Water based ones) allowing the delivery of
Macromolecules through the skin.
No cell injury (i.e. fragmentation of cytoplasm or nucleus, abnormalities of
plasma membrane due to low current intensity used).
(V., Ruggiero, M., and Magnelli, L.: Angiogenesis and the unique nature of tumor matrix. Mol. Biotech. 21: 85-90, 2002.).
Copyright 2008 © Mattioli Engineering All rights reserved
Scientific principle
Cells
Copyright 2008 © Mattioli Engineering All rights reserved
Mattioli Engineering’s Solution
Substances driven
through
Copyright 2008 © Mattioli Engineering All rights reserved
FOR EDUCTIONAL PURPOSES ONLY
Transderm® Meso® System ®
What substances are driven?
•Collagen
•Hyaluronic Acid
•Lidocaine
•L-Carnitine
•Vitamins
•Amino Acids
•Heparin
•Elastine
•Mesotharapy Procedures
•Glutathione
•Hormone Replacement
•Phosphatidylcoline
•Aminophylline
•Hyaluronidase
•MTE-4
•Ionic skin tissue growth gel
•Steroids
•Botulinum toxine (Studies available from May 2007)
•Growth Factors
•Lymidiral
•Veno-M
•Placentex
•Diclofenac
•And many other “cocktails”…..
Copyright 2008 © Mattioli Engineering All rights reserved
Existing Technologies
Why Transderm has been
indicated as alternative to
injection
Copyright 2008 © Mattioli Engineering All rights reserved
Mattioli Engineering’s Solution
Dermoelectroporation®:
Delivery pulses principle
Copyright 2008 © Mattioli Engineering All rights reserved
Delivery pulses principles
Dermoelectroporation
Human use
–
–
–
–
–
(0 - 5mA)
Low and controlled current
No skin damage
Macro molecules deliverable
No adverse effects
No Contraindications
To be used on human beings
Low and controlled
current level applied
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation®
Practicing with
Dermoelectroporation®.
Copyright 2008 © Mattioli Engineering All rights reserved
Mattioli Engineering’s Solution
Dermoelectroporation is a 2 steps Procedure:
It forsees a preventive Microdermabrasion to reduce the skin
impedance and to optimize the use
of
Transderm® Meso® System ®
for transdermal delivery of products
Copyright 2008 © Mattioli Engineering All rights reserved
Mattioli Engineering’s Solution
Copyright 2008 © Mattioli Engineering All rights reserved
Mattioli Engineering’s Solution
Dermoelectroporation®:
Features,Advantages &
Benefits
Copyright 2008 © Mattioli Engineering All rights reserved
Features of Dermoelectroporation®
-
Capability to transdermally deliver ionic drug solutions
-
Capability to transdermally deliver both micro and
macromolecules (greater than 800,000Dalton)
-
No electrolysis of ionic drug solution or variation in the
ionic drug solution pH
-
Both positive and negative ions of the drug are
transdermally delivered at the same time
-
Performing Microdermabrasion first promotes the
transdermal delivery rate
-
Reproducibility of dose delivered
-
Non-invasive
-
No trauma from injections
Copyright 2008 © Mattioli Engineering All rights reserved
Advantages of Dermoelectroporation®
-Transderm fits perfectly in already existing protocols to improve
and shorten outcome (compatibility).
-Transderm allows users to have so many different applications in
only one device.(substances delivered)
-Transderm cancel completely the fear of needles and other
invasive treatments.(Needles free injection system)
-Transderm is the Top N.1 technology FDA cleared as “alternative to
injections”.
Copyright 2008 © Mattioli Engineering All rights reserved
Advantages of Dermoelectroporation®
The Growth of non surgical procedures. (source Aesthetic Buyers Guide 2006 )
“Growth of non-surgical aesthetic procedures is outpacing surgical
procedures by a margin of three to one, with dermal fillers being
only
second to Botox in this category. This demonstrates that the
consumer
shift towards less invasive correction and heavily towards the non
invasive segment.”
“The global dermal fillers market was valued at $442 million in 2005
– an
increase of 25% from the previous year.”
Copyright 2008 © Mattioli Engineering All rights reserved
Advantages of Dermoelectroporation®
BODY SHAPING MARKET
Aesthetic Buyers Guide 2006 –
“The global market for body shaping devices continues to expand strongly.
Over
the next five years, overall sales of body shaping systems will rise 25.1% per
year,
from $182.8 million in 2005 to an estimated $559.9 million in 2010.”
“In 2005, almost 69% of procedure fees were represented by liposuction, a
surgical technique that offers virtually guaranteed removal of small fat
deposits in
a single procedure. However, risks associated with surgery and relatively
long
recovery times will result in minimal procedure growth of just 3.5% per year
for
liposuction, while newer, non-invasive therapies will expand rapidly.”
Copyright 2008 © Mattioli Engineering All rights reserved
Benefits of Dermoelectroporation®
Some of the Benefits Dermoelectroporation® can give:
1)
2)
3)
4)
Patients are not more afraid of any “was-invasive” procedure
No psychological shock in getting the treatment done
No risk of contamination hazard due to needles
Increasing number of treatments per day/month because…
Copyright 2008 © Mattioli Engineering All rights reserved
Benefits of Dermoelectroporation®
Transderm
Increases the
number of
happy patient’s!
..word of mouth!!
Copyright 2008 © Mattioli Engineering All rights reserved
Mattioli Engineering’s Solution
Dermoelectroporation®:
Results
Copyright 2008 © Mattioli Engineering All rights reserved
Rhythids - Canada
Courtesy of Dr. Jaggy Rao, MD University of Edmonton ,Alberta,Canada
Before
After
2 sessions – 2 weeks apart each other
3ml Hyaluronic acid 15mg/ml
Copyright 2008 © Mattioli Engineering All rights reserved
Biopsy on HA - Canada
Courtesy of Dr. Jaggy Rao, MD University of Edmonton ,Alberta,Canada
Stratum Corneum
Epidermis
Dermis
Hypodermis
1.700.000dalton Hyaluronic acid delivery through the dermis to hypodermis using Transderm® ionto system
Copyright 2008 © Mattioli Engineering All rights reserved
FOR EDUCTIONAL PURPOSES ONLY
Cellulite treatment - Italy
Courtesy of Prof. Massirone, President A.M.E , A.M.I.Est. -. Milan Italy
Hairdresser , 44 years old
11
patients - Females.
II,
III and IV level cellulite.
8
consecutive weeks,
once per week session.
1
ml Aminophylline + 2 ml
Lymdiaral + 2 ml Veno-N.
Before
After
Mattioli Engineering © All rights reserved – Do not distribute – For internal use only
Copyright 2008 © Mattioli Engineering All rights reserved
FOR EDUCTIONAL PURPOSES ONLY
Cellulite treatment - Italy
Courtesy of Prof. Massirone, President A.M.E , A.M.I.Est. -. Milan Italy
Shopkeeper, 34 years old
11
patients - Females.
II,
III and IV level cellulite.
8
consecutive weeks,
once per week session.
1
ml Aminophylline + 2 ml
Lymdiaral + 2 ml Veno-N.
Before
After
Mattioli Engineering © All rights reserved – Do not distribute – For internal use only
Copyright 2008 © Mattioli Engineering All rights reserved
FOR EDUCTIONAL PURPOSES ONLY
Cellulite & Adiposity Treatment -USA
befor
e
after
Courtesy of Dr. suneil Jain -. Scottsdale AZ - USA
Cellulite Treatment Protocol:
Delivered every 1-2 weeks until desired result is achieved (usually 3-8 treatments)
Areas are referred to as abdomen, thighs, etc.
Per 5 cc syringe:4 cc Hyaluronidase 300 units/mL+ 4 cc L-carnitine 500 mg/mL+
2 cc Phosphatidylcholine 100 mg/mL
4 syringes used per session.
Copyright 2008 © Mattioli Engineering All rights reserved
FOR EDUCTIONAL PURPOSES ONLY
Post-liposuction correction for
abnormal fat deposition-USA
befor
e
after
Courtesy of Dr. suneil Jain -. Scottsdale AZ - USA
Body Sculpting Protocol:
Delivered every 1-2 weeks until desired result is achieved (usually 2-10 treatment)
Areas are referred to as abdomen, lateral thigh, medial thigh & back.
Per 5 cc syringe: 2 cc L-carnitine 500 mg/mL + 3 cc Phosphatidylcholine 100
mg/mL
4 syringes used per session.
Copyright 2008 © Mattioli Engineering All rights reserved
Lifting - Malaysia
Courtesy of Dr. Chuah, MD Kuala Lumpur - Malaysia
Delivered once for total of 1
treatment.
Prepare solution using a 5cc
syringe filled by Hyaluronic Acid
cocktails through Super PLD.
Ultrapeel microdermabrasion arm test, 2 passes mid lvl power
-followed by hand gommage.
Deliver transdermally HA cocktail
10mins each side medium time
using Transderm® technology.
Apply Calmin Mask for 10
minutes
Apply Basic cream and SPF
cream
Copyright 2008 © Mattioli Engineering All rights reserved
Biopsy with Hyaluronic acid
Courtesy of Dr. Stefania Pacini, MD University of Florence,Italy
Stratum Corneum
Epidermis
Dermis
Hypodermis
800.000dalton Hyaluronic acid delivery through the dermis to hypodermis using Transderm® ionto system
Copyright 2008 © Mattioli Engineering All rights reserved
FOR EDUCTIONAL PURPOSES ONLY
Botulinum toxine for HyperHydrosis
Courtesy of Dr. M.Cavallini , Plastic surgery specialist , Milan , Italy
Test of minor on an
untreated part
Before
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After
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6 cases – all female patients
Sweating measurement with Test of minor
Delivery of 50 units of botulinum toxine per axilla (diluted
with 2ml saline solution)
Time of treatment: 10 minutes
Control after 2 weeks
Test of minor to quantify the result after 14 – 20 days.
Copyright 2008 © Mattioli Engineering All rights reserved
Biopsy with Botulinum toxine
150.000dalton Botulinum Toxin transdermal delivery till muscles using Transderm® ionto system
Stratum Corneum
Epidermis
–Botulinum Toxin magnification
Dermis
Courtesy of Dr. Stefania Pacini, MD University of Florence , Italy
Deep Hypodermis
Scientific complete doocumentation available from April 2007
Muscles
Copyright 2008 © Mattioli Engineering All rights reserved
Mattioli Engineering’s Solution
Dermoelectroporation®:
Summarizing……
Copyright 2008 © Mattioli Engineering All rights reserved
In other words….

Dermoelectroporation technology ……
– utilizes the skin’s water based “channels”
– Increase the skin permeability to allow transdermal delivery of
micro-molecules and macro-molecules
– Controlled electrical pulses delivery (Average current value on the patient is zero).
– No modification of the ionic drug solutions pH and no
electrolysis effect of the ionic solution itself.
– Dermoelectroporation is faster than traditional Ionthophoresis
devices to deliver the same amount of ionic drug solution.
– Dermoelectroporation does not need any skin pHCompensating patch, nor pre-charged compounds, nor carrying
Gel nor others media to get the drug successfully delivered.
Copyright 2008 © Mattioli Engineering All rights reserved
Dermoelectroporation® provides..
Any Questions?
Copyright 2008 © Mattioli Engineering All rights reserved